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F J Veith

Publications and source records attributed to F J Veith.

At least 307 records · Page 17Linked to original sources
PubMed1982

Antigen-induced recruitment of circulating lymphocytes to the lungs and hilar lymph nodes of mice challenged intratracheally with alloantigens.

Little is known about the traffic of lymphocytes within the lung and hilar lymph nodes and even less is known about the alterations in such traffic after immunization of animals via the trachea. To explore these phenomena, we developed an intrapulmonary immune response to alloantigens in mice by depositing semiallogeneic spleen cells into their tracheas. We studied lymphocyte traffic in this model by two assays. In one, we assessed the recruitment of nonspecific 51Cr-labeled resting lymphocytes to the lungs and hilar node and in the other, a dual-antigen, dual-isotope assay, we measured the recruitment of both allospecific and nonspecific labeled blast cells to the lungs and hilar nodes. The results indicated: (1) that circulating T lymphocytes that have recently synthesized DNA (blast cells) are retained in lungs and hilar lymph nodes of challenged mice far more readily than a circulating resting T lymphocytes, and (2) that there is at least some preferential retention of lymphocytes with specific reactivity to the alloantigen used for immunization. Although lymphocyte recruitment to the lungs and hilar nodes was observed during the entire 5-day postchallenge interval, most of the allospecific recruitment occurred during the first 48 hours.

Animals↗
PubMed1982

Fallibility of intra-operative gram stain in the diagnosis of vascular infection.

Three cases of arterial-graft infection are presented. In all cases the gram stain obtained at surgery demonstrated white blood cells but no bacteria. Cultures obtained at operation subsequently grew Pseudomonas aeruginosa in two patients and Bacteroides fragilis in one patient. Failure to recognize the fallibility of the gram stain in the diagnosis of vascular infection may result in incorrect management decisions.

Aged↗
PubMed1982

Lectin-dependent cell-mediated cytotoxicity. A new and simple method to quantitate cytotoxic T cell activity in dogs.

Alloimmune T lymphocytes kill a wide spectrum of target cells, even in the absence of alloantigen recognition, providing that a lectin is incorporated into the assay medium. In these studies we have modified the lectin-dependent cell-mediated cytotoxicity (LDCMC) assay to evaluate the cytotoxic activity of canine T cells using a human B lymphoblastoid cell line (Raji) as the target. Canine peripheral blood mononuclear cells activated in vitro in mixed leukocyte cultures or by concanavalin A (Con A) killed 51Cr-labeled Raji target cells (up to 70% in 4 hr) when Con A was included in the assay. T cells expressed cytotoxic activity only when activated and when con A was present in the assay. Canine T cells activated in vivo also exerted cytotoxic activity in the LDCMC assay. T cells obtained from a rejecting allografted lung by bronchoalveolar lavage displayed considerably higher cytotoxic activity than did T cells obtained from the contralateral normal lung or peripheral blood of the same dog. In contrast, T cells from the lungs and blood of normal dogs and lung-allografted dogs without rejection exerted no cytotoxic activity. We conclude that the LDCMC assay using xenogeneic Raji cells as targets is a valid measure of the cytotoxic activity of canine T cells. Its major advantages include the fact that it is simple to perform and can be used when specific target cells are not available.

Animals↗
PubMed1982

Randomization of autogenous vein and polytetrafluorethylene grafts in femoral-distal reconstruction.

A randomized, prospective, controlled study comparing autogenous saphenous vein and polytetrafluoroethylene (PTFE) grafts in infrainguinal arterial reconstructions has been initiated in three different institutions. A total of 446 operative procedures were divided into six groups, depending on the site of distal bypass insertion into the popliteal or an infrapopliteal artery and on whether the patient received a randomized vein or PTFE graft or an obligatory PTFE graft. The randomized saphenous vein graft patency to infrapopliteal arteries was significantly better (P less than 0.005) at 2 1/2 years than the patency of randomized or obligatory PTFE grafts to the same level. No significant differences between randomized vein grafts and randomized or obligatory PTFE grafts in the femoropopliteal position could be demonstrated up to 2 1/2 years, either above or below the knee. The need for such a study and the limitations of this preliminary report are discussed. The requirement for longer observation of greater numbers of cases is stressed. A current plan for usage of the PTFE graft in the lower extremity is presented.

Adult↗
PubMed1982

Level of amputation after failure of limb salvage procedures.

It has been claimed that the level of amputation is rendered higher by unsuccessful limb salvage bypass procedures. To evaluate this premise, we analyzed 242 major amputations performed for atherosclerotic vascular disease. One hundred and sixty-one amputations were performed as primary operations--50 per cent being below knee and 50 per cent, above knee amputations. Eighty-one amputations followed failure of an arterial revascularization. Twenty-three amputations, 13 below knee and ten above knee, followed late closure of such bypass procedures. Early failure of the bypass in 58 patients resulted in 46 below knee and 12 above knee amputations. After failure of 45 bypasses to arteries distal to the popliteal, 14 of the patients required above knee amputation. Following 28 unsuccessful femoropopliteal bypasses, only two patients needed above knee amputation. Thus, the risk of jeopardizing the knee joint should not be considered a major contraindication to limb salvage procedures, especially if a femoropopliteal bypass is contemplated.

Amputation, Surgical↗
PubMed1981

Spontaneous resolution of popliteal artery thrombosis.

Spontaneous resolution of thrombosis of the systemic arteries is a rarely documented phenomenon. Two patients are described in whom thrombus within the popliteal artery was noted to lyse spontaneously. This resulted in a decrease in ischemic symptoms and was documented by angiography. The spontaneous improvement that occurs in some patients with atherosclerotic femoropopliteal disease may be due to this mechanism as well as to increased flow via collateral vessels.

Aged↗
PubMed1981

Progress in limb salvage by reconstructive arterial surgery combined with new or improved adjunctive procedures.

In the past nine years, 1196 patients whose lower extremity was threatened because of infrainguinal arteriosclerosis have been treated at Montefiore Hospital. In the last six years, limb salvage was attempted in 679 or 90% of 755 patients. Femoropopliteal (318), small vessel (204) and axillopopliteal (29) bypasses were used along with transluminal angioplasty (128) and aggressive local operations to obtain a healed foot. Immediate (one month) limb salvage was achieved in 583 or 86% of the 679 patients in whom revascularization was possible. The 30-day mortality rate was 3%. The cumulative life table (LT) survival rate of all the patients undergoing reconstructive arterial operations was 48% at five years. The cumulative LT limb salvage rate after all reconstructive arterial operations was 66% at five years. The cumulative LT patency rate of femoropopliteal bypasses was not influenced by angiographic outflow characteristics of the popliteal artery but was increased 15% by appropriate reoperations to 67% at five years. Cumulative LT patency and limb salvage rates of small vessel and axillopopliteal bypasses were more than 50% at two years. Of patients undergoing arterial reconstruction, 88% of those who died within five years did so without losing their limbs. Of all the patients in whom limb salvage was attempted, 68% lived more than one year with a viable, useable extremity, and 54% lived over two years with an intact limb. We believe this aggressive approach to limb salvage is justified, and can be undertaken with a low cost in mortality, knee loss and morbidity.

Aged↗
PubMed1981

Cyclosporin A in experimental lung transplantation.

Cyclosporin A (Cy A) has been used in combination with low-dose azathioprine (2 mg/kg/day for 14 days) or other immunosuppressives to treat 13 canine lung allograft recipients. Two of five dogs treated with Cy A and azathioprine survive at 13 and 6 months, respectively, with normal lung function and no evident rejection. The other three dogs in this group survived for over 5 months despite evidence of rejection which was reversed with methylprednisolone (50 mg/kg/day for 3 to 5 days). The addition of prophylactic corticosteroids or their substitution for azathioprine resulted in decreased survival without preventing rejection better. The lung allograft rejection that occurred with Cy A was usually later in onset and more easily reversed by corticosteroids than the lung rejection that occurred with standard immunosuppression. Cy A rejection was also sometimes qualitatively different. Perivascular mononuclear cell cuffs and a proportionally greater decrease in allograft perfusion with respect to ventilation were often more prominent than in rejection with standard immunosuppression. In some instances, decreased allograft perfusion evidenced rejection while the plain chest roentgenogram and ventilation remained normal. Except for infection, which only occurred in animals receiving prophylactic corticosteroids, there was no toxicity from Cy A. These findings indicate that this drug is the safest, most effective immunosuppressive agent yet available for use in lung transplantation.

Animals↗
PubMed1981

Intrafamilial lung xenografts from fox to dog.

The technical feasibility and rejection characteristics of intrafamilial fox-to-dog xenografts were studied. The results indicate that (1) both lungs of a smaller donor can be successfully transplanted into a single hemithorax of a larger recipient, (2) fox-to-dog lung xenografts are capable of providing total pulmonary function at tolerable right ventricular pressures, and (3) the rejection process of unmodified intrafamilial lung xenografts, was similar to and not more vigorous or more rapid than that of canine lung allografts in untreated recipients.

Animals↗