Biomedical subjects

F Hrudka

Publications and source records attributed to F Hrudka.

23 records · Page 2Linked to original sources

PubMedDate not supplied

Ductuli efferentes of the bull-a morphological, experimental and developmental study.

The light and electron microscopic structure of efferent ductules (ED) has been investigated in the mature intact, treated, and immature bulls. On a morphological basis, ED could be subdivided in two major segments. The proximal segment (near rete tests) possesses greater tubular diameter and taller and taller epithelium. Tje epithelium of ED consists of ciliated and nonciliated cells and a smaller number of intraepithelial lymphocytes and macrophages. The nonciliated cells can be distinguished into three different types: type II is characterized by specific granules, type III by vacuoles, whereas type I lacks either granules or vacuoles. Type II and III probably represent two cell species, which differ from each other in their morphological and histochemical properties, while type I may be the "inactive" form of the II and III cells. Type I cells occur throughout ED but they prevail in the proximal region. Type II cells inhabit the proximal segment and type III cells populate and prevail in the distal segment. The structure of nonciliated cells indicates that, in addition to resorptive and carbonic anhydrase activities, they are engaged in marked secretory activity. The control and significance of such an activity is discussed. Developmentally, the difference in tubular diameter in the proximal and distal regions is expressed shortly after birth, the morphological features of resorption were established by 25 weeks, while the formation of granules and vacuoles were not observed until 35 and 50 weeks, respectively.

Aging↗

PubMedDate not supplied

Leukophages in canine semen.

Spermiophages and leukophages were found in teratozoospermic canine semen. Spermiophagy was the role of neutrophilic granulocytes. Leukophages are believed to be oversize macrophages transformed by and released from a chronic inflammatory lesion in the pelvic urethra or the prostate. The term leukophages was adopted to indicate that the cells phagocytize leukocytes, specifically neutrophilic granulocytes. Direct observations on living cells revealed a surprising interaction between these two cell types. It is inferred that neither of the phagocytic cells are related to the disturbed spermiogenesis nor the teratozoospermia.

Animals↗

PubMedDate not supplied

Acrosomo-nuclear syndrome in canine sperm.

An acrosomo-nuclear syndrome in sperm of an infertile semicryptorchid dog is described. Based on an EM study of developing and mature sperm the syndrome is defined by simultaneous occurrence of these symptoms: 1) intranuclear inclusions of acrosomal origin, 2) maldifferentiated apical segment of acrosome, 3) intraacrosomal inclusions of Sertoli cell origin, 4) characteristic change of nuclear shape and 5) retained cytoplasmic droplet. The cause of the syndrome and possibility of a transfer of somatic factors are discussed.

Acrosome↗

PubMedDate not supplied

Microscopical evidence of mycoplasma-like organisms in mycoplasma positive bovine semen.

Contamination with mycoplasma-like organisms was observed microscopically in a bull with impaired sperm quality and positive isolation of Mycoplasma bovigenitalium and ureaplasma. The bull's spermiogram was characterized by decreased initial motility and post-freeze survival rate, primary defects in nucleoplasm, changes in tail conformation and regressive changes of the plasmalemma and acrosome. Vesiculo-lamellar bodies in the periacrosomal region were an accompanying feature. The cytologic picture described is compatible with descriptions reported earlier and related to mycoplasma infection.

Acrosome↗

PubMedDate not supplied

Anomalies of centriolar derivatives manifest in spermatic flagella and respiratory cilia of the stallion.

Anomalies of centriolar derivatives were identified in ejaculates and testicular and tracheal biopsies of a sterile stallion, using light, scanning, and transmission electron microscopy. LM revealed that over half the sperm population had only a vestigial or no tail, while the rest had tails of variable length and shape. The vestigial tail was represented by its anlage, which was implanted on the nucleus and differentiated up to capitulum and collum stage. The stunted tail had an axoneme and its derivatives, but was short in all tail segments. Regardless of the tail length or shape, virtually all axonemes were devoid of the central tubular complex ("9 + 0" defect). Abnormal tail segmentation was associated with missing or defective flagellar sheaths and a profusion of extraneous dense fibers, which contributed to the knobby, bulbous, or lobuliform tail configurations. The gradient of flagellar anomalies seems associated with the inability of the distal centriole to implant on the plasmalemma, to produce the axoneme, or maintain its growth, and to induce the normal differentiation of periaxonemal structures. In contrast to sperm, the tracheal epithelium displayed moderate changes, which are manifest in circumscribed rarefaction of cilia, increased incidence of compound cilia, and disturbed orientation of cilia regarding the plane of central tubular complex. The tracheal cilia were free of "9 + 0" defect.

Animals↗