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Biomedical subjects

F Hoshino

Publications and source records attributed to F Hoshino.

At least 37 records · Page 2Linked to original sources
PubMed1983

[New application of FC-43 as a hypoxic cell sensitizer].

Meth-A fibrosarcoma (10(5) cells) was implanted into the thigh of BALB/c mice. After a predetermined interval, the mice were injected i.v. with FC-43 (artificial blood) with or without oxygen, treated by irradiation or a combination of injection and irradiation and the effect of these treatments was studied. The mear tumor weight of animals treated by irradiation plus oxygen was 6.57 g and 3.18 g in experiments 1 and 2, respectively. Treatment with irradiation plus FC-43 with oxygen resulted in a significant decrease of the mean tumor weight, 3.17 g and 1.59 g in experiments 1 and 2, respectively (p less than 0.05, p less than 0.02). FC-43 with oxygen may have a synergistic effect for radiation therapy. When the preirradiated tumors were small (4.8 mm2), this effect was not obvious.

Animals↗
PubMed1982

Antitumor and interferon-inducing activities of TH69, a whole bacterial preparation of Streptococcus faecalis, in mice.

The antitumor effect of TH69, a lyophilized whole cell preparation of Streptococcus faecalis, was examined in mice using four experimental tumors: Ehrlich carcinoma and sarcoma-180 in DDI mice, MCA tumor in syngeneic C3H mice and Meth-A tumor in syngeneic BALB/c mice. Consecutive intraperitoneal injections of TH69 started one day after the tumor implantation markedly inhibited the growth of the two allogeneic ascites tumors and two syngeneic solid tumors. However, even with the most sensitive Ehrlich ascites tumor, TH69 was ineffective when the tumor cell inoculum was more than 2 X 10(6) cells per mouse. Neither oral nor subcutaneous administrations of TH69 produced significant antitumor effects. After a single intraperitoneal administration of TH69 (300 mg/kg) to mice, interferon was induced in the serum with a titer of 220 U/ml at 14 hr after administration. As TH69 has no direct cytotoxic activity against Ehrlich tumor cells, the antitumor effect of TH69 in mice must be ascribed to some immunopotentiating activity including interferon induction.

Animals↗
PubMed1982

[Characterization and bioactivity of interferon induced by Streptococcus faecalis preparation, TH69].

It was demonstrated that TH69, an lyophylized preparation of streptococcus faecalis TH001 (ATCC No. 31663) induced interferon gamma (IFN gamma) in spleen cell cultures. The IFN activity was found in 8 hrs and reached to the peak of 120-240 U/ml 24-48 hrs after incubation in every concentration of 4, 20, and 100 micrograms/ml of TH69. TH69-induced IFN (TH69-IFN) was produced by T cells assisted by macrophages which were stimulated with TH69, and the activity was neutralized by 80-90% with anti-IFN gamma serum but hardly neutralized with anti-IFN alpha, beta serum. Therefore, TH69-IFN was found to be mostly gamma type. The elution profile of TH69-IFN activity on a column with Toyopearl HW-55S showed that molecular weight of IFN was estimated at 18,000-28,000. Sequential purification on this column of TH69-IFN increased the specific activity to approximately 2.5 X 10(4) U/mg protein (partially purified TH69-IFN: TH69-IFNp). A small amount of TH69-IFNp (5-50 U/ml) augmented NK activity and induced cytotoxic macrophages in vitro. Growth of Meth-A tumor cells exposed to TH69-IFNp of 100 U/ml in vitro declined to 50% and three times injection of TH69-IFN (200 U/50 microliters) into the tumor sites of mice given implants of Meth-A suppressed the tumor growth.

Animals↗
PubMed1978

Roentgeno-histological studies on serial alveolography.

Using the lung obtained from a 22-year-old female at autopsy, roentgeno-histological studies were carried out on serial alveolography. The pulmonary lymphatics were shown to play an important role in the wash-out of the contrast media. The contrast media injected into the alveolus was gradually transferred on and into the alveolar wall and finally entered the subpleural space. Inflow of the contrast media was further noted into the portion probably representing the "juxta-alveolar lymphatic capillary" according to Lauweryns. Since local edema occurs artificially in serial alveolography, the functional aspects of fluid clearance may also be studied simultaneously unlike the conventional alveolography with its emphasis on morphological diagnosis.

Adult↗
PubMed1977

Serial alveolography-preliminary report.

Serial alveolography using a contrast medium for indirect lymphography was developed at our department. With this technique radiograms comparable to so-called barium-imbibed preparations of resected lung are possible. Furthermore, the procedure has the advantage of demonstrating serial radiographic changes as well as the local contrast medium wash-out or clearance, a local functional aspect of the lung, successively in a very short time. It is practical to accomplish simultaneous examinations in both morphological and functional (most probably concerned with the local pulmonary lymph) aspects with the new procedure. Significant differences in radiographic features have been demonstrated between the normal lung and chronic pulmonary emphysema or fibrotic pulmonary diseases.

Adult↗