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Biomedical subjects

F H Wians

Publications and source records attributed to F H Wians.

43 records · Page 3Linked to original sources
PubMed1986

Evaluation of simultaneous measurement of lutropin and follitropin with the SimulTROPIN radioimmunoassay kit.

We evaluated the analytical performance of the "SimulTROPIN" (Becton-Dickinson Inc.) radioimmunoassay for the simultaneous measurement of lutropin (LH) and follitropin (FSH) in human serum. Dose response, linearity, analytical recovery, sensitivity, and reagent stability were all acceptable. Cross reactivity with other structurally related hormones [choriogonadotropin (CG) and thyrotropin (TSH)] was minimal. A normal reference interval was established for nonmidcycle, ovulatory women. We confirm the low degree of CG and TSH cross reactivity with LH and FSH reported by Becton Dickinson and conclude that this assay is an acceptable method for laboratory use in the simultaneous quantification of these analytes.

Adolescent↗
PubMed1985

The effect of osteocalcin on in vitro lipid-induced hydroxyapatite formation and seeded hydroxyapatite growth.

Osteocalcin, the bone gamma-carboxy glutamic acid containing protein, is one of the major noncollagenous proteins both synthesized and localized in bony tissue. Previously, investigators have suggested, based on the in vitro and in vivo properties of this protein, that it may be involved in controlling initiation of mineralization and/or hydroxyapatite (HA) growth. In this study, the in vitro effects of osteocalcin on lipid-induced HA formation, and HA seeded growth were compared. Although osteocalcin inhibited the growth of HA, as indicated by the osteocalcin concentration-dependent decreases in the first order rate constant, kCa, osteocalcin had no effect on lipid-induced calcification, kCa remaining constant at .033 h-1. Binding studies revealed that osteocalcin did not associate with the lipid macromolecules tested (phosphatidyl serine, phosphatidyl inositol, and the Ca-acidic phospholipid-phosphate (Ca-PL-PO4) complexes prepared from these phospholipids) although the protein bound to HA with high affinity. These data suggest that a) osteocalcin is quite distinct from the gamma carboxy glutamic acid containing clotting proteins which have a high affinity for both the acidic phospholipids and for HA, and b) that osteocalcin has little effect on the initial Ca-PL-PO4-dependent formation of HA.

Calcium-Binding Proteins↗
PubMed1985

Imipramine poisoning in a child: lack of efficacy of resin hemoperfusion.

A three-year-old boy ingested up to 1,500 mg of the tricyclic antidepressant imipramine (Tofranil). He entered our facility within two hours of discovery, and multiple resuscitative efforts, which proved unsuccessful, followed. Resin hemoperfusion was used in an effort to remove imipramine from the systemic circulation. Serum concentrations of imipramine and its major metabolite desipramine were determined from serum drawn before, during, and after hemoperfusion. Serum concentrations of imipramine and desipramine did not change appreciably. No improvement in the clinical condition was noted during the hemoperfusion period, which was due in part to the fact that our patient was clinically brain dead upon arrival in our intensive care unit. Our subsequent literature review documents that this case represents the first reported use of hemoperfusion in a pediatric tricyclic antidepressant ingestion, hemoperfusion removes an insignificant portion of the total amount of tricyclic antidepressant ingested, and some pediatric literature misleadingly suggests that hemoperfusion may be useful in such patients. Physicians treating tricyclic antidepressant ingestion cases should avoid using hemoperfusion; standard supportive care remains the essential management response.

Child, Preschool↗
PubMedDate not supplied

Analytical performance characteristics of the T4 method in the Abbott AxSYM immunoassay analyzer.

OBJECTIVE: To evaluate the tetraiodothyronine (T4) method in the Abbott AxSYM immunoassay analyzer. DESIGN: The single-value criteria proposed by Westgard et al. were employed. Assays of control materials established random error (RE) analytical sensitivity, and linearity. Constant error (CE) was established by studying the effects of interferents on T4 quantification. The AxSYM random access immunoassay analyzer was compared with TDx batch analyzer to determine systematic error (SE). Critical concentrations of T4 are 3.0 micrograms/dL and 13.0 micrograms/dL, as these concentrations represent medical decision points. Allowable error at these critical concentrations are 1.0 and 2.6, respectively. SETTING: Peninsula Regional Medical Center Clinical Laboratories. RESULTS: Based on Health Care Financing Administration [HCFA] allowable error goals, RE, SE, and CE were acceptable for concentrations of T4 ranging from 3.0 micrograms/dL to 13 micrograms/dL. PE was virtually absent. Total error (TE) = RE + SE was acceptable at 13 micrograms/dL but exceeded allowable error at the lower critical value of 3.0 micrograms/dL. Abbott AxSYM was sensitive to T4 at a concentration of 0.34 microgram/dL. Linearity was excellent and consistent with the manufacturer's claim over a range 1.0 microgram/dL to 24 micrograms/dL. Of the interferents studied only hemoglobin caused a > 15% change in the measurement of T4. CONCLUSION: The AxSYM immunoasssay analyzer performed to manufacturer's specifications and met HCFA allowable error limits for quantifying T4.

Bias↗