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Biomedical subjects

F H Ruddle

Publications and source records attributed to F H Ruddle.

At least 379 records · Page 21Linked to original sources
PubMed1975

Somatic cell genetic analysis of the interferon system.

Current advances in the use of somatic cell hybrid systems have enhanced the value of these systems for studying eukaryotic cell functions. We have reviewed the use of somatic cells to investigate the human interferon system. It has been shown that interspecific heterokaryons and hybrid cells can produce interferon(s) of both parental types and may be protected from viral challenge by interferon(s) from either parent. Using mouse-human hybrid cells we have assigned a human gene(s) responsible for regulating interferon to chromosome 21 and genes involved in the production of human interferon to chromosomes 2 and 5. Our data also suggest possible assignment of a locus involved in control of interferon production to chromosome 16. Suggested further uses of the somatic cell system for interferon studies include study of the subunit structure of interferons and the development of hybrid lines that produce human interferon at high levels (interferon/somatic cell hybrids/human gene assignment.

Animals↗
PubMed1975

Mammalian chromosome identification in interspecific hybride cells using "Hoechst 33258".

Treatment of fixed chromosome preparations of mouse-human hybrid cells with the benzimidazol derivative "Hoechst 33258" makes it possible to distinguish between mouse and human chromosomes. Treatment of live cells results in differential condensation of mouse and human centromeric regions. The effects of the dye on a mouse-human translocation and the species specific differences of staining and of condensation are in accordance with the assumption that the action of "H 33258" is specifically dependent on the base composition of the DNA.

Animals↗
PubMed1974

Human chromosome 21 dosage: effect on the expression of the interferon induced antiviral state.

Human primary skin fibroblasts trisomic for chromosome 13, 18, or 21 and diploid human skin fibroblasts were induced for an antiviral response with human interferon. The cells that were trisomnic for chromosome 21 were three to seven times more sensitive to protection by human interferon than the normal diploid or trisomic 18 or 13 fibroblasts. The differential response in trisomnic 21 cells is consistent with the known assignment of the human antiviral gene to chromosome 21.

Alleles↗
PubMed1974

Human serum albumin phenotype activation in mouse hepatoma--human leukocyte cell hybrids.

Murine hepatoma cells that secreted mouse serum albumin were fused with human leukocytes that did not produce albumin. The resulting hybrids secreted both mouse and human serum albumin, as demonstrated by immuno-electrophoretic techniques. The activation of the human genome suggests that mapping genes governing specialized functions in somatic cell hybrids may be accomplished by using adifferentiated human cells as a parental line.

Animals↗