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Biomedical subjects

F H Kasten

Publications and source records attributed to F H Kasten.

At least 37 records · Page 2Linked to original sources
PubMed1975

The Feulgen reaction after glutaraldehyde fixation.

A technique is described for performing the Feulgen reaction for DNA on cells and tissues fixed in glutaraldehyde. Blockade free aldehydes by reducing them with fresh 0.5% NaBH4 in 1% NaH2PO4 for 1 hr at room temperature, then rinse in water. Follow by a Feulgen reaction (hydrolysis at room temperature in 6 N HCl for 20 min, Schiff's reagent for 60 min.). Controls assure the completeness and irreversibility of the borohydride blockade. Cytophotometry shows that the DNA content per nucleus is unaffected by the blockade procedure.

Aldehydes↗
PubMed1967

Metachromatic leukodystrophy (sulfatide lipidoses) cultured in vitro.

Cerebral white matter from a patient with metachromatic leukodystrophly was successfully cultured in vitro. A luxuriant growth in Rose chambers and in roller tubes was apparent by the 14th day. The outgrown cells contained large numbers of granules which stained dark brown with cresyl violetacetic acid. The growth and metachromasia were maintained up to 80 days in vitro.

Adolescent↗