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Biomedical subjects

F H Bach

Publications and source records attributed to F H Bach.

At least 361 records · Page 20Linked to original sources
PubMed1980

I-N: a newly described H-2 I subregion between K and I-A.

Strong, primary mixed leukocyte culture-proliferative responses in certain K region-incompatible strain combinations led us to consider whether an I region-like locus might exist between K and I-A. Results obtained with an (A. TL X B10.D2)F1 anti-B10.BR serum provided serological evidence for a new locus; B10.T(6R)-absorbed serum retained reactivity on AQR lymphocytes. This finding demonstrates an antigen, Ia.W41, encoded by a locus, Ia-7, within a new I subregion, I-N.

Animals↗
PubMed1980

Long-term maintenance of "cloned" human PLT cells in TCGF with LCL cells as a feeder layer.

The long-term maintenance of T cells "cloned" by limiting dilution in TCGF was enhanced by the use of irradiated autologous lymphoblastoid cell line (LCL) cells as well as irradiated LCL cells of the individual to which the T cells were originally primed. It was possible to obtain more than 1 X 10(12) cells from a "clone" seeded at one cell per well. Some of the clones tested express primed LD-typing activity.

Cell Division↗
PubMed1980

H-2 I alloantigens and recall of memory cytotoxic responses.

AQR mice were immunized with H-2K and H-2 I encoded alloantigens presented by (Ax6R)F1 splenocytes. Spleen cells from these alloimmune mice were subsequently restimulated in vitro with B10.A lymphocytes and/or B10.T(6R) lymphocytes, thus presenting them with the immunizing H-2K and H-2 I alloantigens independently. When stimulated with B10.A lymphocytes, alloimmune lymphocytes develop significant cytotoxicity against the immunizing H-2K target antigens. When stimulated with a similar number of B10.T(6R) spleen cells, alloimmune lymphocytes undergo a prominant proliferative response, but develop little, if any, cytotoxicity against the immunizing H-2 K target antigens. The most efficient restimulation of cytotoxicity occurs when the alloimmune spleen cells are simultaneously restimulated by B10.A and B10.T(6R) lymphocytes. Stimulation with the immunizing H-2 I alloantigens alone is not sufficient for regeneration of detectable cytotoxic responses from alloimmune spleen populations. Stimulation with the immunizing H-2K alloantigens alone appears to be both necessary and sufficient to stimulate alloimmune cytotoxic responses. Although the immunizing H-2 I alloantigens are apparently not required to generate alloimmune cytotoxic responses, they markedly potentiate the cytotoxic responses induced by the immunizing H-2K alloantigens.

Animals↗
PubMed1980

Hemizygous HLA mutants of lymphoblastoid cells: a new cell type for histocompatibility testing.

HLA variants that have lost expression of multiple cis-linked alleles as determined serologically and enzymatically were analyzed for expression of HLA-D PLT-stimulating (PL) determinants using the primed lymphocyte test. All 19 variants that had lost HLA-DRw3 expression simultaneously had lost expression of the HLA-D region-associated PL specificity. PLT cells made by priming to a variant that was hemizygous for HLA genes resulted in priming to HLA-D PL determinants encoded for by just one haplotype, analogous to priming to an HLA homozygous cell.

Alleles↗
PubMed1980

Gamma ray-induced loss of expression of HLA and glyoxalase I alleles in lymphoblastoid cells.

Gamma rays from a cesium source were used to generate human lymphoblastoid cell line variants that had lost expression of all major histocompatibility complex antigens coded for by a single haplotype. The cell line was heterozygous at the glyoxalase I locus and had the HLA haplotypes HLA-A1, B8, DRw3, and HLA-A2, B5, DRw1. We selected with anti-HLA-B8 antiserum in a population of cells that had been irradiated with 300 R. The incidence of B8-loss variants was 4.1 X 10(-5) on day 5 after irradiation. Analysis of variants showed that expressions of HLA and GLO alleles trans to B8 were retained. However, expression of additional cis-linked HLA and GLO gene products was lost in 12 of 17 variants. Variants that had lost expression of (i) HLA-B8, (ii) HLA-B8, A1, (iii) HLA-B8, A1, DRw3, or (iv) HLA-B8, A1, DRw3 and the cis-linked glyoxalase I allele were obtained. Karyotype analysis was performed on eight variants that had lost expression of two or more cis-linked alleles. Three variants had two normal appearing no. 6 chromosomes, four variants had a deletion that included the region coding for HLA genes on the short arm of one no. 6 chromosome, and one variant had an inversion or translocation involving the short arm of one no. 6.

Cell Line↗
PubMed1980

TCGF production for cloning and growth of functional human T lymphocytes.

In an effort to increase the potency of T cell growth factor (TCGF), several variables were examined for their effects on the production of TCGF. The following manipulations enhanced the potency of TCGF: first, the removal of adherent cells and addition of indomethacin to the producing cultures; second, irradiation with 1000 rads of the cells used to produce TCGF; and, third, the addition of Epstein-Barr virus transformed lymphoblastoid (LCL) cells. It was also noted that the addition of irradiated feeder cells increased the efficiency of limiting dilution cloning.

Cell Division↗
PubMed1980

Family of large surface proteins on human lymphocytes.

Cell surface molecules of T-rosette-positive human peripheral blood lymphocytes were labelled by lactoperoxidase and examined on 5% polyacrylamide gels. Several bands, ranging in molecular weight from 220,000 to 170,000 daltons, were seen. Analysis of the tryptic and chymotryptic digests of the 187K, 200K, 210K and 220K proteins indicated that they are of similar peptide composition. Study of clones derived from this population indicated that individual cells within a population differed in expression of these proteins.

Animals↗
PubMed1980

Requirements for T cells in alloantigen-induced generation of non-T cell-mediated cytotoxicity against syngeneic mouse sarcoma cells.

In vitro stimulation of BALB/c spleen cells with allogeneic normal cells results in the generation of effector cells cytotoxic for syngeneic SV40 virus-induced MKSA fibrosarcoma cells. The conclusion that natural killer (NK) cells are activated that lyse the syngeneic tumor cells derives from findings that 1) treatment of the allostimulated BALB/c effector cells with anti-theta serum and complement does not ablate cytotoxicity against syngeneic MKSA tumor cells and 2) allostimulation of congenitally athymic (nu/nu) BALB/c mice results in significant cytotoxicity against MKSA tumor cells but not against allogeneic target cells. The requirement for the presence of theta-positive cells in alloantigen-induced activation of NK cells cytotoxic for syngeneic tumors was studied. Spleen cells from conventional BALB/c mice are cytotoxic for syngeneic MKSA tumor cells after stimulation with anti-theta serum-treated allogeneic cells or with allogeneic nude cells. In contrast, stimulation of BALB/c nude spleen cells with anti-theta serum-treated allogeneic cells or allogeneic nude cells fails to give rise to anti-tumor effector cells. Thus, theta-positive cells (presumably T cells) are required for alloantigen induced non-T cell-mediated lysis of syngeneic tumors.

Animals↗
PubMed1980

Differential allo-response of murine thymocytes to H- 2 K region different recombinants and to H-2Kb mutants.

Thymocytes used as responding cells in a mixed leukocyte culture with x-irradiated splenic stimulating cells generate highly significant proliferative and cytotoxic responses when responding and stimulating cells differ by the entire H-2 complex. On the other hand, when the genetic difference between responding and stimulating cells is only a K region, very little, if any, proliferative response is detectable and no cytotoxic response is found. In contrast, when responding and stimulating cell donors differ by a spontaneous mutation in the K region of the H-2 complex, as found in B6.C-H-2ba, B6-H-2bd and B6.C-H-2bf, highly significant proliferative and cytotoxic responses can be obtained. These results, thus, argue that the H-2 mutants cannot, with regard to their relationship to the parental strain, be readily equated with a K region difference as defined in the recombinant inbred strains.

Animals↗
PubMed1980

Molecular relationships between large membrane proteins (LMP) expressed on T and B lymphocytes.

Clones prepared from day 5 mixed lymphocyte cultures (MLC) were examined for the expression of large (170,000- to 200,000-dalton) membrane proteins (LMP), found on bulk cultures of resting and allogeneically activated T lymphocytes. Sodium dodecyl sulfate-poly-acrylamide gel electrophoresis (SDS-PAGE) of these proteins indicates both bulk populations and noncytotoxic clones express LMP of similar m.w. Peptide mapping further indicates that LMP of 187,000 (187K) and 200K daltons, found on T cells from bulk cultures or clones and the 220K dalton LMP from B cells, all appear to have a very similar peptide composition. This suggests a single protein (or series of closely related proteins) is differentially processed in functionally disparate populations, and hence may serve as a differentiation antigen for these populations.

Animals↗