Search PubMed⌕ Search

Biomedical subjects

F Gu

Publications and source records attributed to F Gu.

At least 91 records · Page 5Linked to original sources
PubMed1993

Assignment of the linkage group EAM-TYRP2-TPP2 to chromosome 11 in pigs by in situ hybridization mapping of the TPP2 gene.

Restriction fragment length polymorphisms are described for the genes coding for tripeptidyl peptidase II (TPP2) and tyrosinase related protein II (TYRP2) in pigs. A linkage group comprising these loci and the locus for blood group M (EAM) was established by two-point lod score analysis in a three-generation pedigree. Multipoint analysis indicated the linear order EAM-1.1-TYRP2-8.4-TPP2 (recombination distances are given as Kosambi cM). The linkage group was assigned to porcine chromosome 11--the first on this chromosome--through in situ hybridization mapping of the TPP2 gene. TPP2 is the first gene localized on this chromosome using in situ hybridization.

Aminopeptidases↗
PubMed1993

In situ hybridization mapping and restriction fragment length polymorphism analysis of the porcine albumin (ALB) and transferrin (TF) genes.

In situ hybridization analyses were conducted on porcine metaphase chromosomes using porcine liver albumin (ALB) and transferrin (TF) cDNA probes. The ALB gene was assigned to the q12 band of chromosome 8 and the TF gene to the q31 band of chromosome 13. For the latter, a statistically significant secondary peak was observed on the 6p15 band. However, the TF probe predominantly hybridized to the 13q31 band, indicating that this band is the most likely site of the TF gene. Since the TF gene belongs to linkage group V, this linkage group can now be assigned to chromosome 13. The TF and ALB probes were also used for restriction fragment length polymorphism (RFLP) analysis. A screening of 10 unrelated animals revealed TaqI RFLPs for both ALB and TF. Family studies indicated that the ALB and TF polymorphisms were controlled by three and two alleles, respectively.

Alleles↗
PubMed1993

[Effect of serum prolactin levels on luteal function in patients with recurrent abortions].

In order to evaluate the role of prolactin (PRL) on the regulation of luteal function, a series of clinical and laboratory examinations were carried out in 30 patients with history of habitual abortion. The following parameters were included: serum hormone levels by radioimmunoassay during follicular and luteal phase, ultrasonic scan of ovary and endometrial biopsy. The results indicated that: (1) The serum level of PRL was normal in 86.7% of the patients (26/30), and hyperprolactin either hypoprolactinemia accounted for 6.6% of the patients. Both hyperprolactinemia and hypoprolactinemia had abnormal development of follicles and/or luteal function deficiency (LPD) in varying degrees. (2) There were 42.3% of patients with LPD, and abnormal follicular development was one of the important causes for LPD. (3) The concentration of serum PRL showed a marked decrease in patients with spontaneous abortion (P < 0.05) as compared to that of women who went to term. It indicated that PRL level might be associated with luteal function during early pregnancy.

Abortion, Habitual↗
PubMed1992

Detection of c-erbB-2 activation in paraffin-embedded tissue by immunohistochemistry.

Commercially available monoclonal antibodies were tested for their ability to detect increased levels of c-erbB-2 protein in formalin-fixed, paraffin-embedded breast carcinomas. Of five antibodies studied, four (TAB-250, CB11, 3B5, and N3/D10) showed strong cytoplasmic membrane reactivity in 23% (11 of 47) of routinely processed tumors, although interpretation of the immunoreactivity with 3B5 and N3/D10 occasionally was difficult due to cytoplasmic granular staining. Since the c-erbB-2 oncogene is activated by DNA amplification and overexpression of mRNA and protein, the same tumors were analyzed for c-erbB-2 activation by other techniques. c-erbB-2 activation in these 11 tumors was confirmed by immunohistochemistry of frozen tissue (nine of nine tumors), in situ hybridization (nine of 11 tumors), and Southern blot analysis (five of eight tumors). In some of these tumors the failure to demonstrate c-erbB-2 DNA amplification may be due to the small percentage of malignant cells. One additional tumor showed probable c-erbB-2 protein overproduction based on strong immunoreactivity with two antibodies (TAB-250 and CB11), although no definite activation could be demonstrated by additional techniques. Three other tumors (6%) showed equivocal c-erbB-2 protein overproduction based on weak immunoreactivity only with TAB-250, although unequivocal activation could not be demonstrated by additional techniques. The 32 carcinomas (68%) that showed no significant immunoreactivity with any antibodies in routinely processed tissue also showed no detectable c-erbB-2 activation by additional techniques. We conclude that TAB-250 and CB11 are reliable antibodies for detecting c-erbB-2 protein overproduction in routinely processed tissue. TAB-250 also weakly stains a few tumors showing no definite c-erbB-2 activation by other techniques. Two additional antibodies (3B5 and N3/D10) detect c-erbB-2 protein overproduction in paraffin-embedded tissue, but are more difficult to interpret. A fifth antibody, TA-1, is an excellent reagent for use on frozen tissue, but prolonged formalin fixation may impair recognition of its antigenic epitope.

Adenocarcinoma↗
PubMed1992

Chromosomal localization of the hormone sensitive lipase (LIPE) and insulin receptor (INSR) genes in pigs.

Using rat hormone sensitive lipase (LIPE) and human insulin receptor (INSR) cDNA probes, the LIPE gene was assigned to chromosome 6p11-q21 and the INSR gene to chromosome 2q11-q21 in pigs by in situ hybridization. In humans, these two genes are located on the q and p arms of chromosome 19, respectively. The present results provide the first in situ hybridization mapping data for porcine chromosome 2.

Animals↗
PubMed1992

Assignment of the bovine immunoglobulin gamma heavy chain (IGHG) gene to chromosome 21q24 by in situ hybridization.

By the use of in situ hybridization, the bovine immunoglobulin gamma heavy chain (IGHG) gene was assigned to chromosome 21q24. Fifteen percent of the total grains were scored on chromosome 21, with about 65 percent of these grains located on the q24 band. The present results confirm a previous synteny study and provide the precise chromosomal localization of this gene in the cattle genome.

Animals↗
PubMed1992

[Determination of renotropic activity in the sera of 35 uninephrectomy patients].

The renotropic growth factor (RGF) does increase in postuninephrectomy serum although the majority of studies were conducted in experiment. As regard to whether there is postnephrectomy RGF in human being, we have not hitherto reached a conclusion. By incorporating tritiated thymidine (3H-TdR) into primary human kidney cells in culture. We have determined renotropic activity in the sera of 35 uninephrectomy patients. The RGF was found increased in 68% and 76% patients at postnephrectomy days 2 and 5 respectively, showing an average increase of 14% and 33% as compared with that of preoperation level. i.e. The RGF does exist in the sera of uninephrectomy patients. Through multifactorial analysis, it revealed that renotropic activity was higher in male and uninephrectomy patient with better renal function. The same was also found in the patient who had not encountered side effect derived from blood transfusion and that the kidney lesion was benign in nature. Female or senile patient with deteriorated renal function or those with malignant lesion or transfusion side effect showed a lower renotropic activity. But no significant statistic difference was seen between the two categories.

Female↗
PubMed1990

[Hepatic proliferation factor of human fetus liver cells].

It was demonstrated in this study that after ultrasonification and centrifugation of human fetus liver cells the supernatant could increase the incorporation of 3H-TdR into DNA of mice liver cells approximately 2.57 times than that of control. This proliferation effect is not species-specific, but has a relationship with supernatant content. The maximal promotion of 3H-TdR into DNA of the guinea-pig liver cells took place when the supernatant protein content was about 0.55 mg/ml. The supernatant can be divided into four components by Ultrogel A6 chromatography and the fourth component is the dominant both in protein content and in proliferation activity. SDS-polyacrylamide gel electrophoresis showed that its molecular weight is about 15,000 dalton.

Animals↗
PubMed1990

[Chronic active hepatitis with superinfection of delta virus and hepatitis B virus: treatment with Chinese traditional medicine].

Among the 136 patients of HBsAg positive chronic hepatitis admitted from November 1986 to June 1988, 110 patients gave consent to have liver biopsy and out of the 110 liver biopsies 86 were confirmed histologically to be chronic active hepatitis. After screening the 86 patients twice with ELISA (ABBOTT ANTI-DELTA EIA), 26 (30.23%) showed positive results twice. Though it was reported that the incidence of delta hepatitis infection is very low in the HBsAg carriers in Beijing area, the incidence of superinfection of delta hepatitis on chronic active hepatitis B seems to be considerably high (30.23%) as shown in this study. Histological examination revealed that in the liver of patients with superinfection of delta hepatitis on chronic active hepatitis (26 cases) there were more severe changes and more eosinophilic degeneration than in the liver of patients without superinfection (58 cases). The patients were allocated to 3 groups at random. Eleven cases of chronic active hepatitis, with superinfection were treated with Chinese traditional medicine Xiao Chai Hu Tang (XCHT), 5 cases with biphenyl dimethyl dicarboxylate (BDD) and 10 cases with XCHT + BDD. It was noted that after 3 months of treatment, in the XCHT group, HBeAg became negative in 2/3, anti-HBe converted to positive in 2/8 and HBV-DNA converted to negative in 2/2. SGPT became normal in 8/8. XCHT showed a fairly good result and deserves further study.

Drugs, Chinese Herbal↗