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Biomedical subjects

F Gouin

Publications and source records attributed to F Gouin.

228 records · Page 13Linked to original sources

[Evaluation of 1200 venous catheterizations in reanimation].

The authors present a series of 1247 venous catheterizations during resuscitation, the latter being carried out under particularly strict aseptic conditions. This series contains especially: - 364 internal jugular catheterizations; - 223 subclavian catheterizations; - 130 femoral catheterizations. The total number of days of infusion is 6300, of which 3,619 were of prolonged parenteral feeding. 79.5 p. 100 of the catheters cultured proved to be sterile; in the case of those cultures which were positive, 6.5 p. 100 were due to pathogenic organisms, 13.9 p. 100 were due to saprophytes. On the whole, 7 septicemies of venous origin (0.56 p. 100 of all of the catheterizations) were observed. The détailed study of each route shows that the frequency of cultures of pathogen positive catheters is practically the same in all cases. On the other hand, the frequency of cultures of catheters positive with saprophytes is evidently higher for the internal jugular vein; this being particularly true in the male where the role of the beard as a contaminating factor is evoked. The importance of asepsis in the procedure of putting venous catheters in place and in their maintenance seems to be welle demonstrated by the results presented.

Catheterization↗

[Bone resection-reconstruction of the diaphyseal zone in animals. Development of an experimental model, application to the study of biomaterials compared with allografts].

The aim of this work was to develop an animal model of bony resection-reconstruction of the diaphyseal zone and to test the different bone substitutes in comparison with allografts. Eighteen 2-cm diaphyseal resections of the femur were performed in Beagle dogs. Reconstruction was performed using a bone substitute (5 cryopreserved allografts, 5 blocks of polymethylmetracylate with PMH hydroxyapatite, 8 blocks of phosphocalcium ceramic materials with biphased macropores) associated with centromedullar lockek nail. The PMH blocks gave radiographically and histologically unsatisfactory results. Allografts consolidated in two-thirds of the junctions, consolidating with an exuberant periosteal callus contrasting with minimal intracortical repair. No stress fractures were observed despite normal activity. BCP blocks consolidated in three-fourths of the junctions but pseudoarthrosis at the centre of the block was observed in cases in which the fixation was not perfectly stable. Bone occupied 20 and 24% of the surface of the ceramic block in the central and junctional zones respectively. These findings demonstrated the reliability of internal fixation material in the animal model and allowed us to evaluate the different methods for analysing bone repair on histographic and microradiographic images. In addition, the potential of synthetic material such as MBC might be useful in large resections in mechanically stressed zones.

Animals↗

Effect of glutaraldehyde on hemoglobin-dependent quenching of pyrene fluorescence. Application to oxygen diffusion in erythrocyte.

In the present study, we investigate the effect of glutaraldehyde incorporation on the erythrocyte deformability, membrane fluidity and process of molecular oxygen diffusion. The erythrocyte deformability variations were inversely related with the glutaraldehyde concentration incorporated. The membrane fluidity, as assessed by a method based on the kinetics of pyrene dodecanoic acid excimers formation, decreased as the glutaraldehyde concentration increased. So, we verified that glutaraldehyde incorporation was accompanied by membrane rigidification. In the presence of glutaraldehyde, decreased absorption of hemoglobin heme group (420 nm) and decreased hemoglobin-dependent quenching of pyrene butyric acid (PBA) fluorescence would result from hemoglobin polymerization. Using a technique of fluorescence intensity quenching by molecular oxygen (accessibility to oxygen), we showed that oxygen diffusion was decreased in the presence of glutaraldehyde. To conclude, membrane rigidification induced by glutaraldehyde incorporation would affect oxygen molecular process.

Butyrates↗