Search PubMed⌕ Search

Biomedical subjects

F Gottrup

Publications and source records attributed to F Gottrup.

18 recordsLinked to original sources
PubMed1992

Comparison of self reported and observed length, width, and colour of scar tissue.

To evaluate the reliability of self assessment as a substitute for follow up, 30 self reported assessments of length, width, and colour of abdominal scars were compared with those of clinicians. The degree of disagreement between patients and medical observers was not significant. We conclude that as long as instructions to patients are unambiguous, reliable information may be obtained by postal inquiry. Self reported measurements may thus replace time-consuming follow up visits in studies on the influence of various factors on scar tissue.

Abdomen↗
PubMed1991

Arteriovenous shunting and regional blood flow in myocutaneous island flaps: an experimental study in pigs.

In eight pigs, total blood flow, regional capillary blood flow distribution, and arteriovenous (AV) shunting were studied during the first 4 postoperative hours after elevation of a myocutaneous rectus abdominis island flap. Capillary blood flow and AV shunting were measured using radioactive microspheres before flap creation and 1 and 4 hours after surgery. Total blood flow, measured continuously as venous outflow, increased in the first postoperative hour (p less than 0.05). Elevation of the flap caused a slight decrease in skin capillary blood flow (p less than 0.05), whereas muscular capillary blood flow increased (p less than 0.01). AV shunting accounted for 50 percent of the total flap blood flow, whereas it was negligible in the abdominal wall prior to flap elevation. Thus stalk blood flow, skin appearance, and skin temperature may be poor indicators of nutritional capillary perfusion. However, the clinical and nutritional consequences of these findings remain to be established.

Analysis of Variance↗
PubMed1991

Gastric and colonic oxygen tension measured with a vacuum-fixed oxygen electrode.

A new electrode system for determination of visceral oxygen tension is presented. The system consists of a transcutaneous oxygen electrode (E5242 Radiometer A/S. Copenhagen), which is screwed into a suction ring and fixed to the organ by applying a vacuum. The electrode membrane is then in contact with the organ surface via a fluid layer with a thickness of a few micrometres, without interference from atmospheric air and without mechanical disturbance of the membrane. The electrode system was tested on the gastric and colonic wall in six pigs. As reference, a silicone tonometer was placed subserously beneath the vacuum-fixed electrode. The silicone tonometer was connected to a second transcutaneous oxygen electrode. The tissue oxygen tension was changed by subjecting the animals to various inspiratory concentrations of oxygen and to hypovolaemia. The oxygen tension measured by the vacuum-fixed electrode (PO2vac) was compared with the oxygen tension measured by the tonometer (PO2tono). A good correlation was demonstrated between PO2vac and PO2tono, the correlation coefficient being 0.8619, confirming that the suction-fixed oxygen electrode measures tissue oxygen. The lowest possible vacuum to hold the electrode unit in place was between 4.0 and 6.7 kPa. Provided the measuring time did not exceed 3 min. vacuum had only slight influence on the measured oxygen values. The mean change in PO2vac induced by varying the vacuum from 7 to 26 kPa, and 26 to 7 kPa, was only +0.23 kPa (-1.2 to +1.9) and +0.19 kPa (-0.3 to +1.3), respectively. The mean stabilization time, defined as the time from electrode application until achieving 95% of the equilibration value, was 66 sec (SEM, 3.4 sec) and 57 sec (SEM, 3.0 sec) on the gastric and colonic wall, respectively. The measured values of PO2vac varied significantly between different areas on the gastric wall (p less than 0.001). When several surface measurements were performed throughout the investigated area, the mean oxygen tension approached the oxygen tension measured by the tonometer. It is concluded that the vacuum-fixed electrode constitutes a reliable, easy and, noninvasive method for measurements of the oxygen tension in the gastric and colonic tissue.

Animals↗
PubMed1991

The effect of electrical current on healing skin incision. An experimental study.

The effect of electrostimulation on healing of a 6 cm long incision on the dorsum of rats was investigated. Postoperative stimulation was given with direct current (DC) to ten rats, and with alternating current (AC) to ten. Non-stimulated controls were used for each group. The words were biomechanically and biochemically tested three days after the final stimulation. Both DC and AC stimulation caused significant increase of the collagen content around the incision line compared with controls, but the collagen increase did not affect the tensile strength or the energy absorption. Electrostimulation of short duration is concluded to have a minor but significant enhancing effect on the formation of collagen in clean surgical wounds, but no effect on mechanical wound strength in the early postoperative period.

Animals↗
PubMed1991

Tissue oxygen tension in myocutaneous flaps. Correlation with blood flow and blood gases.

The relationship between tissue oxygen tension (PO2) and blood flow, blood gas tensions, oxygen delivery, and oxygen consumption was investigated in myocutaneous island flaps in 24 pigs. The tissue PO2 was measured by the silicone rubber tonometer technique. Capillary blood flow was increased during the first 3-4 hours after implantation of tonometers and they must, therefore, be implanted 3-4 hours before use. After raising the flap, mean (SEM) total flap blood flow increased from 11.2 (0.8) ml/min to 16.0 (1.3) ml/min within the first three hours. Mean (SEM) subcutaneous tissue PO2 decreased from 9.5 (0.6) kPa to 5.2 (0.7) kPa over six hours after the procedure, whereas muscle PO2, blood oxygen tension, and arteriovenous oxygen difference were unchanged. Hence, both the supply to, and use of oxygen in, the flap increased. Muscular and subcutaneous oxygen tensions were about 3 and 1 kPa, respectively, lower than venous oxygen tension. Tissue PO2 did not correlate with blood flow, blood oxygen tensions, arteriovenous differences in oxygen tension, or with oxygen supply in a cross section of animals. Tissue PO2 seems to reflect accurately the balance between oxygen supply and consumption, which might be the most useful indicator for continuous flap monitoring.

Animals↗
PubMed1991

Continuous direct tissue oxygen tension measurement. A new application for an intravascular oxygen sensor.

An oxygen tension measurement equipment, developed for continuous intravascular monitoring was tested as a direct tissue oxygen tension meter. The sterile disposable oxygen probe consists of a Clark type polaro-graphic bipolar electrode placed in a 5 cm long flexible polyethylene catheter (OD 0.55 mm). The probe is easily placed, e.g. in subcutaneous tissue or muscle, with the aid of an introduction needle. In vitro measurements as well as subcutaneous and intramuscular readings were performed. The animals were subjected to various experimental conditions such as different inspiratory oxygen fractions, hypovolemia, and circulatory impairment. Results were comparable to those obtained with previously used and well characterized tissue oxygen tension measurement equipment. The clinical applicability of the oxygen sensor for tissue oxygen measurement was assessed in one patient by 40 hours' surveillance in a free myocutaneous flap. The procedure was simple and implied minimal discomfort for the patient.

Animals↗
PubMed1990

[Conjunctival oxygen tension. Testing oxygen tension measurement of the conjunctiva].

A new apparatus which can measure oxygen tension and temperature in the conjunctiva was tested and compared with measurements of transcutaneous and arterial oxygen tension in eight volunteers in connection with normal oxygen tension, hypoxia and hyperoxia. In hypoxia, the conjunctival oxygen tension was found to be better correlated to and in better agreement with arterial oxygen tension than transcutaneous oxygen tension. The advantages with conjunctival oxygen measurements are continuous measurements in easily accessible and well vascularized tissue with the same arterial supply as the brain. In contrast to transcutaneous measurement, the new method does not require warming of the tissue as the conjunctiva is oxygen permeable.

Blood Gas Analysis↗
PubMed1990

Tissue oxygen tension measurement for monitoring musculocutaneous and cutaneous flaps.

In pigs, latissimus dorsi musculocutaneous island flaps and buttock skin island flaps were raised. Subcutaneous (PscO2) and intramuscular oxygen tension (PimO2) were measured using a non-heated needle electrode before, during and after repeated occlusion of the supplying artery or the draining vein. During arterial and venous occlusion, the tissue oxygen tension in the musculocutaneous flap dropped rapidly. A plateau was reached after 15 min. After arterial occlusion the mean value was 20 mmHg (SEM = +/- 5 mmHg, N = 6) in the subcutis and 16 mmHg in the muscle (SEM = +/- 4 mmHg, N = 10). After venous occlusion the mean value was 11 mmHg (SEM = +/- 3 mmHg, N = 6) in the subcutis. In the skin flap the drop of PscO2 was slower, and after 30 min of arterial occlusion the mean value was 29 mmHg (SEM = +/- 9 mmHg, N = 6). This study has shown that tissue oxygen tension measurement can be used as a sensitive indicator of acute impairment of the supplying vessels in island flaps. The method seems to have potential for monitoring free tissue transfers. A comparable decrease in PscO2 was found for arterial and venous impairment.

Animals↗
PubMed1989

Characterization of the silicone tonometer using a membrane-covered transcutaneous electrode.

A new oxygen tonometry system consisting of a silicone tube, highly permeable to O2 and CO2 is described. The silicone tube was connected to a membrane-covered transcutaneous oxygen electrode (E5242 Radiometer A/S, Copenhagen, Denmark) via an airtight polycarbonate chamber, and flushed with isotonic saline equilibrated with atmospheric air. The present tonometer system offers certain advantages compared with other systems: continuous reading, minimal oxygen consumption, furthermore the system is thermostated and is insensitive to movement. The tonometry system was tested in vitro for characterization of a silicone tube (Coroplast, Fritz Müller KG, Wuppertal, FRG) 1.0 mm in inner diameter and 1.5 mm in outer diameter. The experiments showed that the oxygen tension measured at the electrode after passage of the tonometer approached the oxygen tension outside the tonometer when the length of the tonometer was increased and when the flushing rate of saline through the tonometer was decreased. The time taken for the flushing solution to reach an equilibrium with the oxygen tension outside the tonometer increased with increasing tonometer length, and decreased with increasing flushing rate. Changing the difference between oxygen partial pressure in the flushing solution and the oxygen partial pressure outside the tonometer tube did not influence the relative equilibration value and the equilibration time. When a pO2 value is measured by the electrode, the exact oxygen tension outside the tonometer tube, for every given length of the tonometer and flushing rate through the tonometer can be read from our calibration curves.

Blood Gas Monitoring, Transcutaneous↗
PubMed1989

The effect of cigarette smoking on wound healing.

To see if there was any difference in the skin healing of smokers as opposed to non-smokers we studied 120 women admitted consecutively for laparotomy sterilisation. The method of skin suture was standardised. We compared width, length, and colour of the scars, and assessed the overall cosmetic result using a scoring system in 69 smokers and 51 non-smokers. When the incision was in the midline the scars in the smokers measured 7.4 mm averagely as compared with 2.7 mm in non-smokers (p less than 0.02). There was a corresponding tendency in transverse incisions. When the colour was compared, 26% of smokers had light coloured scars compared with 12% of non-smokers (p less than 0.05). There were no differences between the groups when dark coloured scars were assessed. Overall, using the scoring system, smokers had significantly worse cosmetic results than non-smokers.

Abdomen↗
PubMed1979

Effects of beta2-sympathomimetic on histamine-stimulated gastric acid secretion in dogs.

The effect of a selective beta2-adrenoceptor agonist on histamine-stimulated gastric acid secretion was studied in conscious gastric-fistula dogs. A dose-dependent inhibition was found, most pronounced for the lowest doses of histamine. The inhibition was on the volume, whereas the acidity was unchanged. The pulse rate was increased by the beta2-agonist and by histamine. Propranolol prevented the inhibition of acid secretion as well as the increase in pulse rate. Practolol had no effect on the inhibition of acid secretion but reduced the increase in pulse rate. Dose-response experiments with six doses of histamine and two doses of the beta2-agonist showed unchanged calculated maximal response and an increase in D50. It is concluded that the beta2-agonist inhibits histamine-induced acid secretion in the dog, but to a lesser degree that pentagastrin-induced acid secretion, as found in earlier experiments. The inhibition follows competitive kinetics, and beta2-receptors are probably involved. The influence of beta2-sympathomimetics on the mechanism of gastric acid secretion is discussed.

Animals↗
PubMed1979

Effect of two types of beta-adrenergic blockade on gastric acid secretion during pentagastrin stimulation in non-vagotomized and in vagotomized gastric fistula dogs.

The effect of beta-adrenoceptor blockade by propranolol and practolol on submaximally pentagastrin-stimulated gastric acid secretion was studied in conscious non-vagotomized and in vagotomized gastric fistula dogs. Propranolol (0.5 mg/kg) intravenously augmented gastric acid output in vagotomized dogs, more after truncal and selective vagotomy than after parietal cell vagotomy. Vagally innervated dogs also showed an increase, but to a lesser degree and not statistically significant. The increase restored the acid output to preoperative levels in the vagotomized dogs. Practolol (1.0 mg/kg) intravenously resulted in a slight and insignificant increase in acid output in dogs with truncal vagotomy and had only a negligible effect in vagally innervated dogs and after selective and parietal cell vagotomy. It is concluded that propranolol augments pentagastrin-stimulated acid output in vagotomized dogs, and this augmentation was most pronounced in the totally vagotomized stomach. Practolol had minor influence on gastric acid secretion. This effect of the two beta-blocking agents indicates that beta 2-blockade is most important for the secretory augmentation. The restoration of postvagotomy acid secretion to preoperative levels suggests that adrenergic influence is important for the decrease in pentagastrin-stimulated acid secretion after vagotomy.

Adrenergic beta-Antagonists↗
PubMed1978

Inhibition of gastric acid secretion in dogs by a new sympathomimetic drug.

The effect of salmefamol, a new beta 2-agonist, on pentagastrin-induced gastric acid secretion was studied in conscious gastric fistula dogs. Salmefamol inhibited the acid secretion, an effect which was dose dependent. The volume as well as the acidity was inhibited. Salmefamol caused an increase in the pulse rate. Propranolol prevented the inhibition of acid output as well as the increase in pulse rate. Practolol, a beta 1-adrenoceptor blocker, had no effect on the inhibition of acid secretion but prevented the increase in pulse rate. Dose-response experiments with 6 doses of pentagastrin and 1 dose of salmefamol showed a decrease in calculated maximal response (CMR) and an unchanged D50. It is concluded that salmefamol strongly inhibits pentagastrin-induced acid output in the dog, and the inhibition follows a non-competitive kinetic. The mechanism of gastric secretion probably involves a beta 2-receptor.

Animals↗
PubMed1978

Intralobar pulmonary sequestration. A report of 12 cases.

Intralobar pulmonary sequestration signifies an abnormal and non-aerated mass in the lung, supplied by a systemic artery. Twelve cases are submitted, 10 treated by surgery and two conservatively. There was a male preponderance 11:1, and half the patients were over 25 years. In 66% the abnormality was situated basally on the left side A definite diagnosis can be made only by arteriography, visualizing the number, site, and size of the anomalous vessel(s). Clinically, the symptoms may range from no symptoms at all (four of the present patients) to recurrent pneumonia, cough, and haemoptysis (five of the present patients). In two patients the excised tissue showed epithelial proliferations, tumourlets and slight epithelial dysplasia, but no manifest malignancy. Surgical resection is the only curative treatment for intrapulmonary sequestration. In "silent" cases it is felt justified to apply conservative treatment should primary surgery be contra-indicated.

Adolescent↗