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Biomedical subjects

F Gabl

Publications and source records attributed to F Gabl.

At least 55 records · Page 3Linked to original sources
PubMed1977

Serum-induced changes in electrophoretic mobility of creatine phosphokinase isoenzymes?

BB isoenzyme of creatine phosphokinase (CK-BB) obtained from human brain-extract changes its electrophoretic mobility after incubation in human serum at 37 degrees C. No change of electrophoretic mobility of CK-BB is observed after incubation in isotonic saline. We have shown by means of immunoprecipitation with specific antibodies that the structure of CK-BB is not changed. These findings are supported by other authors and make the diagnostic value of electrophoretic separation of CK isoenzymes doubtful as after a 3-h incubation CK-BB migrates similarly to CK-BB and consequently may be misinterpreted.

Brain↗
PubMed1977

[A comparison of serum phosphohexose isomerase and sialyl transferase activities in patients with malignant disease].

The diagnostic value of serum sialyl transferase, phosphohexose isomerase and lactate dehydrogenase determinations is discussed in respect to patients with malignant diseases. It was found that 80 % of the investigated cases showed an increase in serum sialyl transferase activity. Phosphohexose isomerase was elevated in 46.6 % and lactate dehydrogenase in 23.3% of the cases. The serum sialyl transferase activity was within the normal range in 13.3% of the patients. It is concluded that although the serum sialyl transferase activity is of great diagnostic significance in cases of suspected malignancy, total reliance cannot be placed on the determination of this sole parameter, but only in conjunction with others.

Breast Neoplasms↗
PubMed1977

[Isoenzymes of creatine kinase in the perinatal period (author's transl)].

The serum of new-born children shows a higher catalytic concentration of creatine kinase than adult serum. These values reach normal adult levels within the first 10 days of life. In addition to the MM-, the MB- and BB-isoenzymes of creatine kinase are also found in the serum of women in labour, in cord-serum, and in the serum of newborn children. These findings should be taken into consideration in the analysis of creatine kinase isoenzymes.

Adult↗
PubMed1976

Critical role of the conversion of the third complement component C3 (beta 1C/beta 1A) for its immunochemical quantitation.

For the immunochemical quantitation of the third component of complement C3 the state of conversion of this protein in the sample and the reference serum has to be guarded. Antisera raised against C3 may show different antibody specificity for the antigenic pattern of the C3 proteins which change during proteolysis by activation and also by ageing. It is shown that anti-C3-sera gave differences from -17% to + 91% comparing fresh and aged aliquots of the same sera directly on the same immunodiffusion plate corresponding to a particular anti-C3-serum. The use of three different monospecific antisera directed against the A determinant of C3 seemed to give results with good correlation in respect to C3 conversion. Glass and polystyrene plastic tubes did not have a significantly different effect on the C3 values in respect to C3 conversion. The in vitro acceleration of C3 conversion by thrombin or an inulin-like polysaccharide did not give consistent results in this context.

Complement C3↗
PubMed1976

Creatine kinase isoenzymes in cerebrospinal fluid in a case of brain damage.

We present a case of a 11/2-year-old boy with toxic enteritis, consecutive consumption coagulopathy, and sever brain damage. During the acute phase we found high activity of the BB isoenzyme of creatine kinase in cerebrospinal fluid, but not in the serum. Isoenzyme MM could also be found in the spinal fluid (37.9% of the total activity). We conclude that analysis for creatine kinase isoenzymes in spinal fluid is of clinical importance.

Brain Injuries↗
PubMed1975

[The detection of hyperlipidaemia during routine preventive medicine investigations. Serum lipid values in 3006 viennese adults (author's transl)].

1. 3006 adult volunteers (1001 women after an overnight fast of at least 12 hours and 2005 women and men, 6 to 8 hours after a light breakfast) were investigated in regard to serum cholesterol and triglyceride levels, body weight and height. 2. 73.8% of the men and 75.2% of the women were overweight. The degree of overweight increased with increasing age and only decreased in the advanced age groups. 3. Serum triglyceride and cholesterol values increased with increasing age and showed a decrease in the older groups. 4. Serum triglyceride and cholesterol levels increased with increasing weight, also within each single decade. 5. 21.7% of the 1001 fasting women showed values for TG over 200 mg/dl and/or Chol over 300 mg/dl.

Adult↗
PubMed1975

C3 standards?

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Complement C3↗
PubMed1975

[The isoenzyme pattern of serum alkaline phosphatase in patients on intermittent haemodialysis and kidney transplant recipients (author's transl)].

The total activity and the isoenzyme fractions of alkaline phosphatase were determined in the serum of 15 patients on intermittent haemodialysis and 32 kidney transplant recipients. The isoenzymes of alkaline phosphatase were separated by polyacrylamide gel disc electrophoresis. It was apparent that the increased activity of serum alkaline phosphatase in these patients was not limited to a distinct isoenzyme fraction. 5 of the 15 dialysis patients and 19 (59%) of the 32 kidney transplant patients showed a bone-type isoenzyme pattern. The possible causes for the presence of a bone-type alkaline phosphatase pattern are discussed. The fractionation of alkaline phosphatase gives a valuable indication as to the organ of origin of the particular fraction and is also of diagnostic value when the total activity of alkaline phosphatase lies within the normal range.

Alkaline Phosphatase↗
PubMed1975

Positive direct antiglobulin (Coombs') test caused by cephalexin administration in humans.

Of 71 patients showing a negative direct antiglobulin (Coombs') test (DAT) before administration of cephalexin (CEX) three developed a positive DAT after therapy with this drug (4 percent). No case of immunohemolytic anemia was observed. The sera of the patients with a positive DAT reacted with cephalothin-coated red cells producing high titers, with penicillin cells normal titers and with CEX cells low titers. Since the red cell eluates of these patients did not give significant reactions with red cells sensitized with CEX, cephalothin and penicillin, a nonimmunological mechanism for the development of the positive DAT in these patients has to be assumed.

Adolescent↗