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Biomedical subjects

F G Lehmann

Publications and source records attributed to F G Lehmann.

At least 37 records · Page 2Linked to original sources
PubMed1976

Serum alpha1-fetoprotein in cystic fibrosis.

In 38 children and adolescents with cystic fibrosis, in 19 normal children and 62 healthy adults the serum alpha1-fetoprotein concentrations were measured by radioimmunoassay. In cystic fibrosis patients 97.5% and in normal children 95% of the alpha1-fetoprotein values were within the normal range for healthy adults (1--9 ng/ml). Critical judgement of the reported findings in literature and our own results demonstrate that the investigation of alpha1-fetoprotein in the serum cannot serve for detecting homozygotes of cystic fibrosis genes or heterozygote carriers.

Adult↗
PubMed1976

[Jaundice after aprindin (author's transl)].

Viral-hepatitis-like hepatic damage ("aprindin hepatitis") developed in a 50-year-old woman after taking aprindin, the cause of the jaundice being confirmed by the same signs occurring when the drug was taken again after initial withdrawal.

Aprindine↗
PubMed1975

Immunological relationship between human placental and intestinal alkaline phosphatase.

Crystalline human alkaline phosphatase from placenta and intestine was isolated by butanol extraction, acetone precipitation, a heat step (for the placental enzyme), ammonium sulfate precipitation, anion exchange chromatography, gel filtration and crystallisation with ammonium sulfate. Rabbit antibodies showed a partial cross-reaction between both enzymes in double diffusion, quantitative precipitation experiments and in serial precipitin curves. There was no reaction with human alkaline phosphatases from liver, kidney and bone. Three phenotypes of placental alkaline phosphatase with different electrophoretical mobility exhibited identical immunological reactions with their respective antisera. Monospecific antisera were obtained by absorption with crystalline intestinal or placental alkaline phosphatase. These monospecific antisera against the placental or the intestinal alkaline phosphatase can be used for an immunological determination of these two alkaline phosphatases without contamination by other alkaline phosphatases in human serum.

Alkaline Phosphatase↗
PubMed1975

Immunological methods for human placental alkaline phosphatase (Regan isoenzyme).

Four different immunological methods for the determination of the placental isoenzyme of alkaline phosphatase (Regan isoenzyme) were compared in 64 normal blood donors, 23 healthy laboratory and medical staff workers and 68 pregnant women: a. Inhibition by soluble antibodies to the placental enzyme. b. Precipitation with soluble antibodies. c. Precipitation with immobilized antibodies. d. Measurement of the activity of the placental alkaline phosphatase following binding to an immunoabsorbent that has been obtained by polymerization of anti-placental-alkaline phosphatase gamma-globulin using glutaraldehyde. The immunoabsorbent method yielded the best results. The optimal conditions were evaluated for the measurement of the activity of immunoabsorbent-fixed placental (tumoral) alkaline phosphatase activity. This method was applied to 209 normal blood donors and to 239 patients with different malignant tumors: 25.5 percent of the cancer patients exhibited an elevated Regan -isoenzyme activity in the serum.

Alkaline Phosphatase↗
PubMed1975

Early detection of hepatoma: prospective study in liver cirrhosis using passive hemagglutination and the radioimmunoassay.

(1) Passive hemagglutination and radioimmunoassay are suitable methods for the detection of AFP in the low concentration range. (2) In 3.72% of the cases a clinically unknown carcinoma was found in an unselected group of patients with liver cirrhosis. (3) 21.9% of the patients showed AFP elevations up to 2000 ng/ml. In 10.6% of this group, increasing titers demonstrated a primary liver cell carcinoma. In 89.4% a transitory rise of AFP was not associated with tumor growth. Levels return to normal values within three months in 90% of the cases. (4) Transitory AFP elevations are not correlated to clinical conditions (praecoma, coma, delirium, bleeding, ascites, shunt) or to biochemical parameters (GOT, GPT, bilirubin, prothrombin complex time, gamma-globulin). (5) A temporary rise in AFP is more frequently observed in groups with high hepatoma incidence than in groups with low hepatoma incidence. (6) Therefore, it may be suggested that a transitory rise of AFP could reflect a "primary reaction" of carcinogenesis. (7) Primary liver cell carcinoma is found to be more frequent in posthepatitic than in postalcoholic, cryptogenic, and other cirrhosis and to be more frequent in australia-antigen positive than in australia-antigen negative cases. (8) Routine serological tumor antigen screening of patients with a precancerous disease is useful.

Carcinoma, Hepatocellular↗
PubMed1975

[Regan-isoenzyme of alkaline phosphatase in sera of cancer patients (author's transl)].

The Regan-Isoenzyme of alkaline phosphatase was determined immunologically measuring the antibody-fixed tumor alkaline phosphatase activity. In 68 blood donors the normal range varied from 0.1145 to 0.6351 (delta E/20 min at 405 nm, chi = 0.3748, standard deviation = 0.1013). 28.9% of 83 patients with different carcinomas showed a pathologically elevated Regan-Isoenzyme activity in the serum. The percentage of positive results as compared to previous reports is higher due to a more sensitive technique.

Alkaline Phosphatase↗