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Biomedical subjects

F Faure

Publications and source records attributed to F Faure.

At least 73 records · Page 4Linked to original sources
PubMed1988

[Magnetic resonance imaging of high anorectal malformations. A preliminary study apropos of 15 cases].

High level ano-rectal anomalies were evaluated by Magnetic Resonance Imaging (MRI) in 15 patients. One neonate and two infants were evaluated preoperatively. Sagittal slices identified properly the blind rectal end in the 3 cases. Associated anomalies (tethered spinal cord, right ectopic kidney in 1 case, recto-urinary fistula in another case, upper excretory dilatation in 2 cases) were found during the same examination. In the post-operative period (12 cases), major data were obtained on axial transverse slices. When pulled-through intestine is outside the levator sling, re-do-surgery can be indicated. MRI appears as an accurate tool to assess these anomalies.

Adolescent↗
PubMed1987

A novel subset of human lymphocytes with a T cell receptor-gamma complex.

We have previously characterized a CD3+ T cell receptor (TCR) alpha/beta- human fetal cloned cell line, termed F6C7, which surface-expresses a CD3-associated gamma chain identified by anti-NKFi, an mAb with a restricted clonotypic reactivity. Here, we have produced an additional antibody, anti-Ti-gamma A, which recognizes a public epitope of the gamma molecule defined by anti-NKFi. Ti-gamma A is present on approximately 3% of circulating lymphocytes with a wide range (1-15%) among 30 healthy individuals tested. Two-color immunofluorescence experiments performed with anti-Ti-gamma A and BMA 031 mAb (a reagent specific for the TCR-alpha/beta receptor) showed that surface expression of Ti-alpha/beta and Ti-gamma A is mutually exclusive. Moreover, it was found that most Ti-gamma A+ cells are CD2+, CD3+, CD4-, CD5+, NKH1-, HLA class II-negative. In contrast, the expression of the CD8 molecule on these T lymphocytes appears to be variable from one individual to another. Finally, we found that Ti-gamma A+ cells represent a majority of peripheral lymphocytes that express CD3 proteins but not the TCR-alpha/beta heterodimer. The delineation of this unique lymphocyte subset should help further studies on the biology of cells with a CD3-associated gamma complex.

Animals↗
PubMed1987

Cloned human CD3- lymphocytes with natural killer-like activity do not express nor rearrange T cell receptor gamma genes.

Here we report the characterization of T cell receptor (TCR) gene activation in 14 CD3- cloned lymphocytes with natural killer-like activity from different origins. No transcription of either alpha or beta or gamma genes is detected in any of these clones. All J beta, C beta clusters are in germ-line configuration. The 5 presently described J gamma loci (J gamma P1, J gamma P, J gamma 1, J gamma P2, J gamma 2) are also in germ-line configuration on each chromosome. These results indicate that several distinct populations of CD3- cloned NK cells do not use gamma gene products as a recognition structure. Perhaps more importantly, these cells do not appear to be engaged in the earliest presently known stage of T lymphocyte differentiation, namely rearrangement at one of the J gamma loci.

Antigens, Differentiation, T-Lymphocyte↗
PubMed1986

Natural killer clones derived from fetal (25 wk) blood. Probing the human T cell receptor with WT31 monoclonal antibody.

We have conducted a phenotypic and functional analysis of 19 cloned cell lines generated after allogeneic stimulation of circulating lymphocytes from a normal human fetus aged 25 wk. Using a limited series of mAbs (Anti-T3, WT31, T4, T8, and NKH1A), cloned cells were found to fall in three groups. Three clones have a conventional "inducer" phenotype. Three clones have a phenotype (T3+, WT31+, T8+, and NKH1A+) similar to that of certain NK active mature T lymphocytes present in adult peripheral blood. In contrast, 13 cell lines display surface characteristics that have not been described previously. Indeed, they express T3 proteins but not the WT31 determinant. In light of previous studies, these results show that WT31 mAb is a unique reagent directed at an invariant epitope of the human T cell receptor that is not present on all circulating T3+ fetal lymphocytes. Functionally the T3+, WT31+, and NKH1A+ clones were found to kill immunizing LAZ 388 cells, as well as K562, while T3+, WT31- and NKH1A+ clones display NK-like function exclusively. Moreover, only WT31+ lymphocytes present in the cell line used for cloning experiments have the capacity to recognize alloantigen-bearing cells. Together, these data suggest that expression of WT31 may be necessary for recognition of alloantigens, while NK reactions mediated by T3+ lymphocytes are WT31-independent.

Animals↗
PubMed1986

Noninvasive fluorine-19 NMR study of fluoropyrimidine metabolism in cell cultures of human pancreatic and colon adenocarcinoma.

Fluorine-19 NMR spectrometry was used to monitor the metabolism of two antineoplastic fluoropyrimidines, 5-fluorouracil (5FU) and 5'-deoxy-5-fluorouridine (5'dFUrd), in cell cultures of human pancreatic (Capan-1) and colon (HT-29) adenocarcinoma. The preliminary results showed, for the two tumor cell lines treated with 5FU, the presence in nonperfused cells of three signals corresponding to intracellular metabolites: 5FU, F-nucleotides and F-nucleosides. When the cells were perfused only the signals of F-nucleotides and 5FU were present. The F-nucleosides observed during the analysis of the nonperfused cells came from the conversion of F-nucleotides. During the NMR recording of Capan-1 cells at 37 degrees C the first metabolite of the catabolic pathway of 5FU, 5,6-dihydro-5-fluorouracil, occurred. At the beginning of the NMR recording of Capan-1 cells treated with 5'dFUrd, two signals corresponding to F-nucleotides and F-nucleosides (consistent with 5'dFUrd) were observed; during the analysis, a supplementary signal corresponding to 5FU appeared. Even after pretreatment with methotrexate the signal of 5FU incorporated into RNA was not detected. Our experiments, performed in attempts to observe the signal of the ternary complex between thymidylate synthetase (TS), 5-fluoro-2'-deoxyuridine-5'-monophosphate (FdUMP) and 5,10-methylene-tetrahydrofolate (5,10-CH2FH4), allowed detection in some cases of a broad signal, whose chemical shift was similar to that reported in the literature following incubation of TS with FdUMP and 5,10-CH2FH4, but our results were not always reproducible.

Adenocarcinoma↗
PubMed1986

[Digitalized angiography of the thorax in children].

Technical aspects and indications for the use of digital subtraction angiography of thorax in 109 children are discussed, and the interest of this exploratory method emphasized in chronic respiratory disease in children. Results obtained are compared with other investigations for screening of bronchopulmonary dysplasia. Although generally reliable for exploration of thoracic aorta anomalies (coarctation and abnormal vascular arch) it is considered to be incompletely effective for investigation of congenital heart disease.

Acute Disease↗
PubMed1986

[Echographic aspects of candidiasis of the urinary tract in infants. Apropos of 2 cases].

Predisposing factors in two children with urinary tract candidiasis included: neonatal period, uropathy with malformation, massive antibiotic therapy. Ultrasound images showed dilated excretory cavities of variable echo intensities and without posterior shadow cone. Diagnosis was confirmed by urine culture and a satisfactory outcome obtained by specific therapy and surgery for malformations.

Candidiasis↗
PubMed1986

[Enterocystoplasty in the treatment of neurological bladders. Can the results be improved?].

Over a period of 3 years. 10 cases of enterocystoplasties have been done in congenital neurologic bladder in children. Seven patients have been operated using sigmoide (6), caecum (1) in their tubular form. In the last 3 cases sigmoide was used as a patch without any resection of the bladder. Post-operative urodynamic testings were better with sigmoide patch. The authors pointed out this technique.

Adolescent↗
PubMed1985

Tn7-encoded proteins.

Proteins encoded by Tn7 have been studied in Escherichia coli maxicells harbouring either various deleted ColE1::Tn7 plasmids or Tn7 fragments cloned in pBR322. Six Tn7-encoded proteins were detected and named p18, p32, p40, p54, p85-a and p85-b according to their apparent molecular weight. Protein p18 is dihydrofolate reductase type I and p32 is probably the protein conferring resistance to streptomycin/spectinomycin. Both genes map on the left-hand part of Tn7. The genes for the four other proteins are located on the right-hand part of Tn7. We propose that they fully cover a 6.9 kb DNA fragment without any overlapping. Starting from the right-hand end towards the middle of the transposon, these four genes are in the following order: p85-a, p54, p40 and p85-b. Transposition of Tn7 onto E. coli plasmids requires the proteins p85-a, p85-b, p54 and p40. However, transposition onto the chromosome does not require the p85-b and p40 products.

Bacterial Proteins↗
PubMed1985

[Striated and delayed nephrography].

About a case of striated and delayed nephrogram seen on a diabetic child, authors come back to the different etiologies. Among them, the tubular precipitation of Tamm-Horsfall protein seems to be given like on the right possibilities. Whatever is its etiology, the mechanism of striated appearance is always the same, being founded on the radiated disposal of the collecting ducts and on a tubular stasis beeing with iodine concentration.

Adolescent↗
PubMed1984

Pseudotumoral cecum after hydrostatic reduction of intussusception.

The authors report two cases of intestinal intussusception successfully reduced by hydrostatic pressure. A post-reduction filling defect in the cecum disappeared spontaneously on follow-up barium enema, and is therefore consistent with cecal edema. It appears an initial period of close observation for such cases is worthwhile, delaying the decision about surgery until after a follow-up barium enema; this may confirm the disappearance of the filling defect.

Barium Sulfate↗
PubMed1984

[Multilocular cystadenoma of the pancreas. An uncommon tumor in the child].

A 9-month-old infant presented with a multilocular pancreatic cystadenoma. Pathological features of this extremely rare lesion in children are outlined and the relevant literature reviewed. Diagnostic methods are analyzed, and emphasis placed on the difficulty of diagnosis of non-renal multicystic abdominal masses by ultrasound imaging in children.

Cystadenoma↗
PubMed1979

[Traumatic coronary-cardiac fistulas. Apropos of a case].

The case of a 24-year old patient having suffered a bullet wound was reported. After a hemopericardium accompanied by minor signs of tamponade, a continuous murmur gradually appeared due to a fistula between the right coronary artery and the right atrium, which was strictly symptomless: surgical repair was performed eight years after the accident. This satisfactory spontaneous outcome, after surviving the life threatening danger of tamponade and myocardial infarction was also observed in the 21 cases of coronary-cardiac fistulae already published. The fistula, which almost always involves the right heart cavities, only gives rise to a continuous murmur which is often discovered at a later stage. The long term course of traumatic coronary-cardiac fistulae can only be assessed by comparison with congenital coronary-cardiac fistulae, which are much better known, and the consequences of which are on all accounts similar. Four complications are discussed: heart failure, coronary insufficiency, infectious endocarditis, and the rupture of the fistulized coronary artery due to aneurysmal dilatation. These risks are statistically small and more theoretical then real, and the justification of systematic repair of traumatic coronary-cardiac fistulae now rests essentially on the relatively low risk of the procedure.

Adolescent↗