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Biomedical subjects

F Eb

Publications and source records attributed to F Eb.

At least 55 records · Page 3Linked to original sources
PubMed1992

Chlamydial endocarditis: a report on ten cases.

Over the period 1983-1990, 10 cases of infective native-valve endocarditis as a result of Chlamydia were seen. All patients were men, with a mean age of 42 years, and none had a history of exposure to Chlamydia psittaci. Symptoms, such as weight loss and anorexia, with fever in eight cases, had persisted for at least 2 months before admission. Haemodynamic failure was present in seven patients, and neurological signs in four. The aortic valve was involved in seven cases, the mitral valve in one and both valves in two. Vegetations, often fingerlike, were observed by echocardiography in nine cases. All patients required valve replacement, and three died in the year following diagnosis. Blood cultures were consistently negative in all cases, and no antibiotics had been given before admission. Significant titres of complement fixing anti-chlamydial antibodies were present in six cases, and micro-immunofluorescence using the three chlamydial species showed cross-reacting antibodies in all nine cases studied, with transient IgM positivity in six cases. Histologically, the leaflets were fibrosed and infiltrated by large macrophages containing dark red granules after Machiavello staining. These granules were specifically stained by immunofluorescence using monoclonal antibody to Chlamydia common antigen, but not by that specific for C. pneumoniae. No organisms were seen after Gram staining, and no positive chlamydial immunofluorescence was seen on sections of valves from patients with staphylococcal or streptococcal endocarditis.

Adult↗
PubMed1991

Use of ultrasound to increase infection of McCoy cell monolayers by Chlamydia trachomatis strain.

An ultrasound cell disrupter with a cooled cup tip was used to increase rapidly Chlamydia trachomatis infection in vitro. After three growth cycles of the NI-1 strain (serovar E), the pulsed ultrasound use enhanced the number of infected McCoy cells by approximately 12-times, as compared with control; and 8.8-times over the shaking with glass beads and centrifugation technique. After three growth cycles of the fast-growing LB-1 strain (serovar L2), the enhancement was by 15 and 10.8 respectively. Consequently, ultrasound treatment with a cooled cup tip can offer a working standard procedure to increase rapidly the number of cells infected with Chlamydia.

Bacteriological Techniques↗
PubMed1990

Immunological specificity of monoclonal antibodies to Chlamydia psittaci ovine abortion strain.

Fifty-one monoclonal antibodies were prepared by two different techniques against Chlamydia psittaci strain A22 isolated from an ovine abortion. These antibodies were tested for reactivity by the indirect immunofluorescent antibody technique with eleven reference Chlamydia strains (nine C. psittaci, one Chlamydia trachomatis and one Chlamydia pneumoniae). Four classes of specificity were recognized for monoclonal antibodies: genus, species, subspecies and type specificity. The type-specific monoclonal antibodies were non-reactive with ovine arthritis isolates. Twenty monoclonal antibodies were specific for two mammalian strains: ovine abortion A22 and K mouse. Some monoclonal antibodies were reactive with C. pneumoniae strains and non-reactive with C. trachomatis strains. All these monoclonal antibodies were very useful for improving the diagnosis of chlamydial infection, the antigenic analysis and the serotyping of C. psittaci.

Abortion, Veterinary↗
PubMed1990

Chlamydia trachomatis peritonitis: another cause of protein-rich lymphocytic ascites.

Ascites as a main feature of Chlamydia trachomatis infection in two young women was successfully treated with doxycycline. In both patients, ascitic fluid protein levels and lymphocyte counts were very high. This report, as well as six previously published cases, suggest that ascitic fluid culture for C. trachomatis and blood antibody determinations should be performed in all cases of protein-rich lymphocytic ascites in sexually active women.

Adult↗
PubMed1989

[Biochemical characteristics and antigenic structures of Chlamydia].

New biotechnology in immunology and molecular biology has enabled the identification and definition of the structure of glycolipids and especially membrane proteins of Chlamydia. Chlamydia antigen lipopolysaccharide, major outer membrane protein, protein 74 kDa, eukaryotic cell binding protein and cysteine rich proteins are all carriers of antigenic determinants, genus, species or type specific. They are very usefull for diagnosis of Chlamydial infections and epidemiological studies. These membranous antigens have an important role in the pathogenesis of these bacteries. Finally these studies have contributed to the isolation of a new species: C. pneumoniae (TWAR strains).

Antigens, Bacterial↗
PubMed1989

[The role of the bacteriology laboratory in the epidemiologic study of nosocomial infections].

The knowledge and the control of nosocomial infections is based upon clinical and bacteriological data. The bacteriology laboratory is an important source of informations as more easily supplied than data are treated with computer. Results of the laboratory allow the control of bacteriological flora and sensitivity (or resistance) for each species and for each antibiotic, in each department and in the entire hospital.

Bacteriological Techniques↗
PubMed1989

[Prevention of nosocomial infections at a cardiovascular surgery unit: therapeutic tactics, efficacy and results].

The experience of prevention of nosocomial infections inside a cardiovascular surgical unit leads to insist, among the various tactics, on the quality and the mode of disinfection of the operating room and the intensive care unit, in particular, the technics of air and surface processing (by Anios) of sterilizing water (by Hyconium B and Filtranios), of sterilized respirators (by Formobloc). Quality and efficiency are bacteriologically controlled. The good results, obtained for 3 years, testify of the efficiency of such technics. However some architectural and structural drawbacks can jeopardize a strict compliance towards prevention of nosocomial infections.

Cardiovascular Diseases↗
PubMed1988

[Clinical and pharmacokinetic study of ceftriaxone in the treatment of 14 episodes of ascitic fluid infection].

A clinical study and dosages of ceftriaxone were performed during the treatment of 14 ascitic fluid infections. Fourteen cases of ascitic fluid infection were treated with ceftriaxone at doses of 2 grams daily. The isolation of the causative organisms was obtained in 11 out of 14 cases by systematic culture of the ascitic fluid in hemoculture bottles with aerobic and anaerobic medium. Of 11 evaluable infectious episodes ten were cured. Ceftriaxone diffuses rapidly in the ascitic fluid because, within the first hour; mean concentrations were already of 18.6 micrograms/ml largely exceeding the usual MIC for these organisms. A good tolerance for the drug, the well spaced rythm of infections preventing unnecessary parenteral treatment in cirrhotic patients, and its effectiveness against Enterobacteriaceae justify its choice in the treatment of ascitic fluid infections with sensitive organism.

Aged↗
PubMed1988

[Chlamydia trachomatis perihepatitis].

Fifteen observations of perihepatitis due to Chlamydia trachomatis were studied. Occurring in young women (M: 29, 3 years), genital infection is seldom simultaneous, sometimes not even found in the case history (5 times out of 15). Women consult for a biliary type of pain (11 cases out of 15), associated with pelvic pain (2 cases). The diagnosis of perihepatitis is suspected on the normality of usual further investigations, and confirmed by laboratory tests: rarely by direct sighting (2 cases out of 8) but each time with a positive serology and a titer of antibodies higher than 1/64 by the micro immunofluorescence method. Treatment by Cyclins (13 cases) or Macrolids (2 cases) leads to the regression of the painful symptoms during the first week.

Adolescent↗
PubMed1985

[Our experience in the serodetection of Chlamydia infections in tubal pathology].

Chlamydia appears to be an increasingly frequent cause of salpingitis and its complications (sterility, extra-uterine pregnancy). The difficulty of isolating Chlamydia in cell cultures has led us to study the value of serology in the form of immunofluorescence and ELISA. This study is based on 475 cases (206 controls and 269 women with tubal disease). Although it is difficult to interpret an isolated IgG level, the results nevertheless confirmed the value of serodetection: especially the comparison between the control group (18.44% positive) and the pathological group (62.08% positive) and the effectiveness obtained when cases of tubal sterility were treated with appropriate antibiotics, alone or in combination with surgery.

Animals↗
PubMed1984

Spontaneous Yersinia enterocolitica peritonitis in idiopathic hemochromatosis.

We report a case of spontaneous Yersinia enterocolitica peritonitis revealing idiopathic hemochromatosis in a 62-yr-old man with fever and diarrhea. Blood and stool cultures were repeatedly negative, but Yersinia enterocolitica serotype 9 was isolated from ascitic fluid and serum agglutinins against this organism were positive at a dilution of 1:2560. Moreover, a serum Brucella agglutination test was positive at a dilution of 1:320. Antibiotic therapy with doxycycline led to a rapid clinical improvement, with disappearance of the organism in the ascitic fluid. This case suggests that Yersinia enterocolitica infection must be suspected in any febrile abdominal syndrome occurring in patients with iron overload, such as thalassemia major or idiopathic hemochromatosis.

Ascitic Fluid↗