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Biomedical subjects

F E Cox

Publications and source records attributed to F E Cox.

At least 19 recordsLinked to original sources
PubMed2001

Concomitant infections, parasites and immune responses.

Concomitant infections are common in nature and often involve parasites. A number of examples of the interactions between protozoa and viruses, protozoa and bacteria, protozoa and other protozoa, protozoa and helminths, helminths and viruses, helminths and bacteria, and helminths and other helminths are described. In mixed infections the burden of one or both the infectious agents may be increased, one or both may be suppressed or one may be increased and the other suppressed. It is now possible to explain many of these interactions in terms of the effects parasites have on the immune system, particularly parasite-induced immunodepression, and the effects of cytokines controlling polarization to the Th1 or Th2 arms of the immune response. In addition, parasites may be affected, directly or indirectly, by cytokines and other immune effector molecules and parasites may themselves produce factors that affect the cells of the immune system. Parasites are, therefore, affected when they themselves, or other organisms, interact with the immune response and, in particular, the cytokine network. The importance of such interactions is discussed in relation to clinical disease and the development and use of vaccines.

Animals↗
PubMed1998

Control of coccidiosis: lessons from other sporozoa.

Coccidiosis is the most important parasitic infection in poultry worldwide and also causes problems in cattle, sheep and goats. Control is largely limited to good husbandry and prophylactic chemotherapy using a range of drugs against which resistance is rapidly acquired. Attempts at vaccination using conventional vaccines have been disappointing and there is now a need for a new approach. Research into the immunology of coccidiosis has lagged behind that of other sporozoans and there are useful lessons that might be learned from studies on toxoplasmosis, cryptosporidiosis, theileriosis and malaria. In these infections the emphasis has turned to the cytokine network that drives the response towards protection. Central to these studies are the roles of interferon-gamma, interleukin-12 and activated macrophages with the involvement of nitric oxide in parasite killing. Cytotoxic T cells have also increasingly been implicated. Research has shown that different immune responses can be elicited by manipulating the cytokine system and these new concepts can be applied to the design of peptide or recombinant vaccines, and the possibilities of developing such vaccines against coccidiosis will be discussed.

Animals↗
PubMed1997

Designer vaccines for parasitic diseases.

Conventional ways of developing vaccines against infections, either on pragmatic grounds or by identifying protective antigens and attempting to mimic natural immune responses, have largely been unsuccessful for parasitic-infections, mainly because of the complexity of the immunological processes involved. It is clear that a new approach is required and it is now known that the "immunological environment" in which the immune response is initiated is as, or more, important than the actual antigens used. CD4+ and CD8+ T1 cells, through the agency of IL-2 and IFN-gamma, direct the response towards cell-mediated immunity involving cytotoxicity and macrophage activation, whereas T2 cells, through the agency of IL-4 and IL-10, direct the response towards antibody production. The two poles are counter-regulatory in that IFN-gamma inhibits antibody formation and IL-4 and IL-10 inhibit macrophage activation. However, immune responses are not immutable and can be artificially driven towards one or other pole, for example IFN-gamma, IL-2 and IL-12 favour T1 responses, whereas IL-4 and IL-10 favour the T2 type. With this knowledge, it is possible to design recombinant or nucleic acid vaccines that include gene products or genes for desirable cytokines as well as the appropriate antigen. For example, in experimental leishmaniasis, protective immune responses can be induced by the incorporation of genes for IL-2 and IFN-gamma into recombinant Salmonella typhimurium vectors and nucleic acid vaccines. A similar approach might be appropriate in experimental schistosomiasis, in which exogenous IL-12 drives the immune response towards the T1 pole and ameliorates T2-mediated pathology. These approaches require novel delivery systems and these have already begun to produce encouraging results. However, simply modifying the nature and route of administration of the vaccine is not enough and attention has now turned to the effector molecules involved, for example nitric oxide, and the signaling systems that are modified by the presence of particular cytokines.

Animals↗
PubMed1997

Killing of blood stage Plasmodium vinckei petteri by spleen macrophages through L-arginine dependent mechanism.

A series of experiments was carried out to investigate the involvement of the L-arginine-dependent effector mechanism (LADEM) in the killing of the blood stages of the rodent malaria parasite, Plasmodium vinckei petteri, by activated spleen macrophages in vitro. P.v.petteri-infected red blood cells were co-incubated with spleen macrophages from normal mice which had previously received 10(8) Mycobacterium bovis (BCG) 5 days earlier, in the presence of 0.1 microgram/ml LPS with and without 0.1 mM L-NMMA, an L-arginine analogue which inhibits LADEM, for 16 hours. The viability of the parasites was assessed according to their infectivity following inoculation into experimental mice. Incubation of parasites with spleen macrophages in the presence of LPS without L-NMMA reduced the parasite viability to about 3%. When L-NMMA was included in the culture, inhibition of parasite killing was observed, resulting in an increase of parasite viability to about 21%. These data provide evidence to suggest that spleen macrophages play an important role as effector cells in the immune mechanisms against P.v.petteri infection, and that the parasite killing of these cells, at least in part, was mediated by LADEM.

Animals↗
PubMed1994

The evolutionary expansion of the Sporozoa.

The sporozoans comprise a coherent group of protozoans, with characteristic and complex life cycles, containing 4-5000 species parasitic in invertebrates, particularly annelids and arthropods, and vertebrates. The group is a very successful one but neither its origins nor evolution are well understood. Considerations of traditional life cycles combined with newer methodologies have thrown some light on the evolutionary expansions of the main groups of sporozoans, the gregarines, coccidia, haemosporidians and piroplasms. The sporozoans of economic importance such as the coccidia, malaria parasites and piroplasms have received most attention but the data obtained have also thrown new light on the possible evolution of less well studied groups and it is concluded that conclusions based on simple comparisons of life cycles will have to be modified. It is also clear that humans have played a major part in affecting the distribution and present abundance of many sporozoans of economic significance and probably also those of less importance, and that the rates of evolutionary expansion are much more rapid than previously thought.

Animal Population Groups↗
PubMed1992

Interactions between chemotherapy and immunity in bovine theileriosis.

In bovine theileriosis the use of chemotherapy to control an infection sufficiently long to permit the establishment of a solid protective immune response has been developed as a routine vaccination procedure. Infections with Theileria parva and T. annulata can be prevented by the administration of carefully controlled numbers of sporozoites simultaneously with a long acting tetracycline and this form of immunization has been widely used for the control of East Coast fever in Africa with considerable success. In this review, the nature of the chemotherapy, the immune response and the interactions between chemotherapy and immunity in the development of infection-and-treatment immunization procedures are discussed.

Animals↗
PubMed1991

Malaria vaccines--progress and problems.

Developing a vaccine against malaria is a major priority of the WHO. A decade of research exploiting the techniques of molecular biology has yielded a series of potentially protective vaccines. However, progress has been frustrated by the complexity of the parasite's life cycle and the antigenic diversity exhibited by each stage. Although candidate vaccines are now entering human trials, questions still arise concerning the nature of a successful malaria vaccine and who will benefit from it.

Amino Acid Sequence↗