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Biomedical subjects

F Cotton

Publications and source records attributed to F Cotton.

42 records · Page 3Linked to original sources
PubMed1998

Separation of human globin chains by micellar electrokinetic capillary chromatography.

A new separation method of human globin chains by micellar electrokinetic capillary chromatography (MECC) is described. In this method, a 25 mM phosphate buffer (pH 2.5) containing 7 M urea and 1% (w/v) reduced Triton X-100 buffer system was used. All experiments were performed in a 47 cmx50 microm I.D. uncoated fused-silica capillary. The separation voltage was set at 19 kV. Normal globin chains derived from normal adults and newborns, alpha, beta, delta, Ggamma and Agamma globin chains as well as common variant globin chains were successfully separated within 20 min. High reproducible migration times of globin chains (CVs of intra- and inter-assay were less than 1% and 2% respectively), and quantification of Ggamma and Agamma chains (CVs for intra- and inter-assay were less than 5% and 10%, respectively) were obtained. This new MECC method provides primary information on structural modification of globin chains. It can be an important diagnostic tool in clinical laboratory practice in the field of hemoglobinopathies.

Adult↗
PubMed1998

Protein and enzyme patterns in the fluid cavities of the first trimester gestational sac: relevance to the absorptive role of secondary yolk sac.

The potential absorptive role of the yolk sac membrane was evaluated by examining protein and enzyme patterns in embryonic fluids and by comparing the synthetic capacity of the secondary yolk sac, fetal liver and placenta for human chorionic gonadotrophin (HCG) and alpha-fetoprotein (alphaFP). In yolk sac fluid samples, protein electrophoresis showed two main electrophoretic bands with mobilities comparable to those of albumin and interalbumin-alpha1-globulin, and immunoblotting revealed the presence of albumin, alphaFP, alpha1-antitrypsin, alpha2-macroglobulin, transferrin, complement factors 3 and 4 and immunoglobulin G. In coelomic fluid, similar results were obtained, except for the absence of alpha2-macroglobulin and the presence of ceruloplasmin and IgA. After electrophoresis and immunoblotting with specific antibodies, beta-HCG was detected in all placental homogenates and culture media but was not revealed in any of the corresponding yolk sac tissue samples. Reverse transcription-polymerase chain reaction (RT-PCR) showed that all placental samples express beta-HCG mRNA whereas all yolk sac and liver samples express alphaFP mRNA. These findings suggest that the yolk sac membrane is an important zone of transfer between the extra-embryonic and embryonic compartments and may also help to further develop therapeutic protocols making use of fetal somatic gene therapy by injecting transduced cells into the exocoelomic cavity.

Absorption↗
PubMed1998

A simple method for carbohydrate-deficient transferrin measurements in patients with alcohol abuse and hepato-gastrointestinal diseases.

Carbohydrate-deficient transferrin (CDT) is known to be increased in alcohol abuse. Several methods were developed for its measurement (e.g. isoelectric focusing with Western blotting or immunofixation, anion-exchange chromatography followed by immunoassays). We describe a greatly simplified isoelectric focusing technique which does not require immunofixation. CDT results obtained with this method were compared to other biological markers of alcohol abuse, i.e. mean corpuscular volume (MCV), aspartate aminotransferase (ASAT) and gamma-glutamyl-transferase (GGT), in 55 patients distributed in three groups (i.e. healthy control subjects, control patients suffering from various hepato-gastrointestinal diseases and alcohol abusing patients). Sensitivity and specificity were 33-89%, 61-57%, 89-49% and 83-100% for MCV, ASAT, GGT and CDT, respectively. We conclude that our method is highly suitable for routine clinical use.

Alcoholism↗
PubMed1993

[A cutaneous parasitosis rarely observed in France: chromomycosis].

The authors report a case of chromomycosis presenting like a tumoral lesion in a patient coming from the Isle de Mayotte. The causative agent is Fonsecaea pedrosoi. The evolution was favorable after surgical treatment. The epidemiology, the histopathological features, the diagnosis problems and the evolution of the illness are studied.

Adult↗
PubMed1990

Quality control, or just control?

This article presents an editorial perspective on the dichotomy of state dental association/licensing boards' subjecting out-of-state oral health professionals to clinical board exams, while at the same time pursuing the possible introduction of preceptorship training for dental hygienists. Reciprocity and licensure to perform all functions for which the operator has been educated to competency are appropriate avenues to pursue. If licensing bodies are truly interested in quality control, then preceptorship should not be sought, because it is inconsistent with that goal. From an educational perspective, when dental assistants and dental hygienists are capable of obtaining licensure through this model, apprenticeship programs for dentists are logically the next step. Licensing bodies may then become redundant, and the professionalism of the oral healthcare community brought into question. Issues of reciprocity and preceptorship have nothing to do with quality control--they have to do with the outright control of one profession over another.

Dental Hygienists↗