Biomedical subjects

F Campos

Publications and source records attributed to F Campos.

41 records · Page 3Linked to original sources

PubMed1987

Purification, cDNA Cloning, and Developmental Expression of the Nodule-Specific Uricase from Phaseolus vulgaris L.

Nodule-specific uricase (uricase II) from Phaseolus vulgaris L. was purified to homogeneity by chromatographic methods. Purification data indicated that uricase II is approximately 2% of the total soluble protein from mature nodules. Specific antiserum was raised and used to determine the developmental expression and for immunoselection of polysomes. Uricase II was antigenically detected early in nodule development, 2 to 3 days before nitrogen fixation. Uricase-encoding cDNA clones were isolated by hybridizing a nodule-specific pUC9 cDNA library with labeled mRNA from immunoselected polysomes and a 35,000 molecular weight uricase II-encoding cDNA from soybean. An homologous clone (pNF-UR07) was used to assess the expression pattern of the specific transcript during development. Northern-blot analysis indicated that uricase II mRNA is exclusively expressed in nodule tissue.

Journal Article↗

PubMed1986

Expression of Two Different Glutamine Synthetase Polypeptides during Root Development in Phaseolus vulgaris L.

Immunoprecipitation and two-dimensional gel electrophoresis analysis of the glutamine synthetase (GS) polypeptides (alpha and beta) during Phaseolus vulgaris root development shows that the alpha polypeptide is the main component of the enzyme in the embryo and in up to 5 day old roots. From 5 days on, the beta polypeptide becomes the root predominant GS monomer. The alpha/beta ratio of the in vitro translated GS polypeptides from the total polysomal RNA isolated at different root ages correlates with the alpha/beta ratio observed in the root extracts. These results suggest that the two root GS polypeptides are encoded by different mRNA species in Phaseolus vulgaris.

Journal Article↗

PubMed1984

Assessment of marginal vitamin A deficiency in Brazilian children using the relative dose response procedure.

Vitamin A status was determined using fasting plasma levels and the relative dose response (RDR) procedure before and 30, 120, and 180 days after administration of an oral massive (200,000 IU) dose of vitamin A. The study was carried out in Recife, Brazil among 93 children of 7 yr or less from low income families who attended two day-care programs. The RDR procedure is conducted by obtaining a fasting blood (A0), feeding 450 retinol equivalents and obtaining a second blood specimen after 5 h (A5). The RDR-(A5-A0)/A5 X 100. A single massive oral dose of vitamin A was given after conducting the base-line RDR test. The RDR procedure was repeated at 30, 120, and 180 days. Weight and height measurements were obtained at each observation and the bloods were analyzed for Hb, total protein, and serum iron, as well as vitamin A. Serum albumin was determined in 120- and 180-day bloods. Serum iron levels were improved 30 days after supplementation with the massive dose of vitamin A. The RDR procedure was found practical to apply in mildly undernourished children under nonclinical condition. In this group of low income children presumed to be habitually ingesting minimally adequate diets, a serum vitamin A level of 20 micrograms/dl or less invariably was associated with an elevated RDR test. Blood levels between 20 to 40 micrograms/dl were not consistently predictive of the RDR response. All elevated RDR tests reverted to normal after supplementation with vitamin A, presumably indirectly indicating a presupplementation inadequate vitamin A status. Hence, the RDR was a more sensitive indicator of inadequate vitamin A status than was only a serum level of vitamin A when blood levels were above 20 micrograms/dl.(ABSTRACT TRUNCATED AT 250 WORDS)

Body Weight↗

PubMed1981

Pharmacologic manipulations of brain catecholamines and the behavior of Callithrix jacchus (marmoset).

In experiment 1, groups of marmosets were injected with 50 - 200 mg/kg L-dopa, 0.5 - 2.0 mg/kg apomorphine (AP), or 0.5 - 5.0 mg/kg methamphetamine. The number of head movements, number of body movements, number of times the tongue was extended, and the time the animal remained in an upright posture were scored during a 90-min period after the injections. L-Dopa and AP induced large dose-dependent increases in body movements and time in upright posture. Furthermore, the smallest dose of AP induced self-destructive behavior. The main effect produced by methamphetamine was a constant turning of the head with the body remaining still. In experiment 2, the marmosets were treated with alpha-methyl-p-tyrosine (AMPT) or haloperidol prior to challenge with AP or methamphetamine. Haloperidol blocked most of the effects of both drugs, while AMPT blocked only methamphetamine effects. In experiment 3, marmosets were injected with increasing doses of haloperidol for 22 days. Upon withdrawal of the neuroleptic, methamphetamine and AP effects were not increased, suggesting absence of supersensitivity of dopaminergic receptors.

Animals↗

PubMedDate not supplied

Interleukin-6 polymorphisms and the risk of cervical cancer.

Recent data implicate that cytokine gene polymorphisms are important in pathogenesis of various neoplastic and nonneoplastic human diseases, and it was recently suggested that polymorphisms in interleukin (IL)-6 might increase the risk of gynecological malignancies, including cervical carcinomas. The aim of this case-control study is to compare the IL-6 polymorphisms in cervical cancer patients and healthy controls and to assess whether any of these polymorphisms would increase the risk of developing cervical cancer. The material in this case-control study consists of 56 patients with cervical carcinoma and 253 population-based control subjects, all ethnic Brazilian women. Control subjects were cancer-free women, following a negative cervical cytology and colposcopy. IL-6 genotyping was performed using a polymerase chain reaction-based restriction fragment length polymorphism. Distribution of the GG, GC, and CC genotypes in cases and controls was significantly different (P= 0.033). Compared with the GG genotype as reference, the adjusted odds ratio for the combined GC and CC genotypes in cancer patients was 1.90 (95% confidence interval, 1.1-3.4). These data suggest that women carrying at least one C genotype in their IL-6 promoter region (-174G-->C) are at higher risk of developing cervical cancer.

Adult↗