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Biomedical subjects

F C Sitzmann

Publications and source records attributed to F C Sitzmann.

At least 37 records · Page 2Linked to original sources
PubMed1986

Microcirculation and hemorheology of children with type I diabetes.

A group of 53 children, suffering from diabetes mellitus type I and a group of 12 healthy children were compared. In both groups the following data were determined: Haemoglobin A1C, retinal blood flow (video fluorescence angiography), erythrocyte velocity in finger nailfold capillaries (video capillaroscopy), capillary tortuosity, plasma viscosity, erythrocyte rigidity, and haematocrit. The degree of capillary tortuosity was significantly elevated and erythrocyte velocity was significantly diminished in the group of diabetic children in comparison to the healthy children. Regarding rheological data there was a significant decrease in erythrocyte deformability for the diabetic children. Diabetic children with good stabilization presented better rheological parameters than the poorly stabilized ones. Although these findings provided sufficient information, it will be necessary to cover a long follow up period to judge the prognosis of good and poorly stabilized diabetic children.

Adolescent↗
PubMed1986

Determination of chymotrypsin in stool by a new photometric method.

A photometric method to determine chymotrypsin in stool, equivalent to the titrimetric analysis, was developed. The chymotrypsin concentrations found in healthy children and those with gastrointestinal and pancreatic disease permit the same clinical conclusions as the titrimetrically determined results. In view of its lower technical requirements, this method is suitable for the identification of maldigestion in pediatric and general practice.

Adolescent↗
PubMed1986

[Behavior of glucosephosphate isomerase in children with malignant diseases].

The normal range of glucose-phosphate-isomerase (GPI) in the plasma of children during the first month of life is up to 80 U/l; until the end of the second year of life between 11 and 50 U/l; thereafter the upper limit is 46 U/l. In osteogenic sarcoma or medulloblastoma there is a good correlation between activity of GPI in plasma and clinical tumor stage. In a lot of other tumors sensitivity of this enzyme is either very low as in Ewing-sarcoma or myeloic leukemia or there is no consistent relation to the extent of the tumor. High activities of GPI are equally obtained in children suffering from cystic fibrosis, diabetes mellitus or muscular dystrophy. GPI is not valid as a tumor marker even being raised in sarcoma and medulloblastoma as mentioned. So it is not necessary to check GPI activity as a part of routine enzyme chemistry.

Adolescent↗
PubMed1985

Generalised glucosephosphate isomerase (GPI) deficiency causing haemolytic anaemia, neuromuscular symptoms and impairment of granulocytic function: a new syndrome due to a new stable GPI variant with diminished specific activity (GPI Homburg).

A new glucosephosphate isomerase (GPI) variant is described which is characterised by very low specific activity in erythrocytes, granulocytes and muscle tissue, nearly normal stability, normal kinetic properties and a decreased electrophoretic mobility. The propositus suffers from a complex syndrome involving erythrocytes (congenital haemolytic anaemia), granulocytes (decreased production of superoxide anion and reduced bactericidal activity in vitro) and the neuromuscular system (myopathy, mental retardation). It is suggested that the clinical syndrome results from generalised GPI deficiency due to a decreased specific activity of the variant enzyme, which cannot be compensated by an increase of de-novo synthesis of GPI protein even in cells exhibiting active protein synthesis such as granulocytes and muscle cells.

Adolescent↗
PubMed1985

[Clinical significance of the measurement of serum enzyme activity in children].

The most frequently estimated enzyme-activities in the serum of children are discussed; these are the serum transaminases GOT and GPT, gamma-GT, alkaline phosphatase, lactate and hydroxy-butyrate dehydrogenase, as well as the creatinkinase and the alpha-amylase. The limited significance of these results for the diagnosis or prognosis is shown. A pattern of several enzyme-activities allows for a better judgement of a situation than the measurement of only one enzyme. Nowadays easier isoenzyme determinations allow for an organ-specific diagnosis. The inherited rise of alkaline phosphatase activity in the absence of disease is known, equally the idiopathic rise of creatinkinase and alpha-amylase. All results of enzyme-activity measurements should be judged in the context if the clinical situation.

Adolescent↗
PubMed1985

[Determination of chymotrypsin in the feces by a new photometric method].

Fecal chymotrypsin (FCT) was determined in stool specimens of healthy children and those with gastro-intestinal disease, by a new photometric method. The values are comparable with chymotrypsin concentrations found by pH-stat method. The new test is cheap, reliable and easy to perform. For this reasons and for the sensitivity of all tubeless pancreatic function tests (NBT-PABA, FDL, FCT) is rather low (60-70%), the FCT-test may be preferred as diagnostic marker for differential diagnosis of exocrine pancreatic insufficiency.

Child↗
PubMed1985

[Significance of the measurement of serum substrates in children].

Almost daily very many analyses are performed in the clinical laboratory, even if their significance for a given diagnosis is uncertain or unnecessary. In this paper the significance of Na, Cl, Ca, Mg, P, total protein, urea nitrogen, creatinine and uric acid is discussed, as well as methodological aspects, age-dependent reference values, and especially the relevance of these laboratory results for the diagnosis. Each laboratory result represents only a symptom, which may lead to some diagnosis, but the main importance remains with the clinical symptoms.

Adolescent↗
PubMed1984

[Erythrocyte metabolism in type I diabetes mellitus (key enzymes of glycolysis)].

The three important key-enzymes HK, PFK, PK may be influenced by insulin. We have, therefore, measured these enzyme activities in the erythrocytes in a total of 119 childrens suffering from juvenile diabetes type I, 44 of them with a good state of metabolic control and 75 with a bad control. In both groups the activities of the three enzymes were not significantly different. Compared with the reference values, PFK and PK, were mild diminished, HK slowly elevated in both groups of diabetics. We don't find remarkable changes which could be responsible for a shortened red cell life span. The mechanism of hemolysis in diabetics is probably caused by oxidant damage to the membrane.

Adolescent↗
PubMed1984

[Immunoreactive trypsin in the serum of normal children and children with gastrointestinal diseases].

Serum immunoreactive trypsin (IRT) was measured in cord blood and blood specimens of 156 healthy children of different age. These results were compared with the IRT of children with gastrointestinal disease. While IRT from newborn is significantly elevated, mean trypsin levels form older children do not differ from those found in adults. In acute pancreatitis too, as in renal failure, trypsin is elevated. Low trypsin values were estimated in acute hepatitis and Crohn's disease. In cystic fibrosis (CF) serum trypsin levels depend on the exocrine function of the pancreas. The IRT assay on dried blood-spots, seems to become a reliable and convenient neonatal screening test for CF in newborns.

Acute Disease↗
PubMed1983

[N-acetyl-cystein-(NAC)-activated creatinkinase (CK) and isoenzyme CK-MB in the serum of children].

We have examined the variation of creatinekinase levels (NAC-activated) with age in 170 children. The subjects included 40 neonates, 18 premature neonates, 40 small babies, 32 infants and 40 schoolchildren. The enzyme activity of CK-MM was very high in the first hours after delivery and remained high for a few days. The isoenzyme MB in healthy newborns also showed a higher catalytic concentration. These values (about 2-12 U/l) reached normal levels of adults within 4 months of life (0.5-5 U/l). The same rule applied to CK-MM: enzyme activities of 160 U/l and more in the first days of life declined to 16-75 U/l during the first 4 months. No correlation between birth trauma and the increase in serum-CK was found. Because of the increased CK-MM (and CK-MB) found in normal newborns screening for Duchenne-type muscular dystrophy should be postponed for a few weeks after delivery. In view of the relatively high endogenous serum CK-MB in the neonates (release of CK-MB from the skeletal muscle) the test lacks the specificity for cardiac damage. Intramuscular injections of several drugs lead to a distinct increase in CK activity. A rise of CK-MM was seen 4-24 h after catheterization of the heart.

Acetylcysteine↗
PubMed1982

[New culture method for the diagnosis of bacteriurias].

The aim of this study is to check the clinical relevance of a new cultural method for the detection of urinary tract infection especially in urine samples containing residue of antimicrobial agents. The new procedure concerns a urine collecting and cultivating cup (UCCC Biotest Serum Institute), containing a recovery agar (RA5) with incorporated chemotherapeutica antagonists. The efficiency of the UCCC is compared with the familiar dip slide coated with cystein lactose electrolyte deficient (CLED) agar and MacConkey agar. A collective of 305 children is considered in this study. The investigation was carried out before starting, three to five days after starting and three to five days after stopping the chemotherapy. The highest correlation between the microbial count in urine samples and the whole clinical diagnosis was achieved with RA5. The lowest correlation was attained with MacConkey agar. With CLED agar a good correlation was determined before starting therapy, a modest correlation during therapy and very good correlation after stopping therapy. Regarding relapses and reinfections the detection quote of relevant bacteriuria during therapy amounted to 90% as RA5 was used, 62% with CLED agar and 34,5% with MacConkey. RA5 proved very useful for follow-up purposes, especially during treatment with antimicrobial agents and may be considered as a susceptibility testing in vivo.

Adolescent↗
PubMed1982

Interstitial de novo deletion of the long arm of chromosome 5: mapping of 5q bands associated with particular malformations.

A new case of interstitial deletion of the long arm of one chromosome No. 5 (q13 leads to q22) is described. The girl shows mental retardation, severe hypotonia, dysmorphic facies and peculiar dermatoglyphics. The relationship between partial trisomies and partial monosomies of 5q chromosomal segments and associated clinical features is discussed. It seems possible to draw a rough phenotypic map of the long arm of chromosome 5 (5q), correlating observed malformations and phenotypic features with specific chromosomal regions.

Abnormalities, Multiple↗
PubMed1982

[Erythrocyte enzymes and 2,3-diphosphoglycerate in juvenile diabetics].

The activity levels of phosphoglyceromutase, Glucose-6-P-dehydrogenase. 3-P-Glyceratkinase and Glutathionreductase of the erythrocytes as well as 2,3-Diphosphoglycerate were determined in a total of 263 children suffering from juvenile diabetes mellitus. They were divided into two groups: 103 diabetics with a good state of metabolic control and 103 diabetics with a bad control. The results of 57 diabetic children were rejected. The enzyme activities have been shown to vary. PGM activity was increased in all diabetics, G-6-PDH only in such with bad condition of metabolic control. The activity of 3-PGK was significantly diminished, Glutathionreductase activity was indifferent in both groups. Until today we don't found results of other authors determining these enzymes. The changes of some enzyme activities in diabetics may be due to hormonal mechanisms by insulin.

2,3-Diphosphoglycerate↗
PubMed1981

[Trypsin deficiency in diabetes mellitus of children and adolescents (author's transl)].

In 111 children and juveniles aged 7--27 years suffering from insulin-dependent diabetes mellitus the immunoreactive plasma human pancreatic trypsin was measured by means of the method RIAGnost-Trypsin-test, Behring. Decreased plasma trypsin was measured at the onset of diabetes already. In patients with diabetes with a period of 8--13 years the trypsin values were found to be significantly lower (79.6 +/- 35.3 ng/ml) than in these suffering from diabetes 0--3 years (100,4 +/- 36.9 ng/ml). None of all these patients had clinical pancreatic disease. Abnormalities in exocrine pancreatic function occur in patients with insulin-dependent diabetes mellitus, which is not confined to pancreatic islets only. Until today clinical consequences not can be drawn.

Adolescent↗