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Biomedical subjects

F C Hay

Publications and source records attributed to F C Hay.

87 records · Page 5Linked to original sources
PubMed1976

Routine assay for the detection of immune complexes of known immunoglobulin class using solid phase C1q.

A new, sensitive and quantitative technique for the routine estimation of immune complexes has been devised. The assay involved the binding of immune complexes to Clq linked to the surface of plastic tubes; the amount of immune complex bound is then determined by adding radiolabelled anti-human IgG. Immune complexes were detected in twelve out of thirty-one patients with systemic lupus erythematosus. The test should prove useful in the diagnosis of immune complex diseases and will be valuable in the evaluation of disease activity.

Antigen-Antibody Complex↗
PubMed1976

Changes in immunoglobulin class and subclass of anti-DNA antibodies with increasing age in N/ZBW F1 hybrid mice.

The immunoglobulin class and subclass distribution of antibodies to double-stranded (ds) DNA has been determined in NZB/W F1 hybrid mice by a double antibody radioimmunoassay. The results show that there are marked age- and sex-related variations in the class and subclass distribution of these antibodies. In young mice of both sexes, the predominant anti-ds DNA antibody was of the IgM class, but as the animals aged, there was an increase in the level of the IgG2a and IgG2b antibodies. An apparent switch from predominantly IgM to IgG antibody occurred in females before males and at about the age of onset of overt immune complex disease in these animals. The possible significance of these results in the pathogenesis of the murine lupus syndrome is discussed.

Aging↗
PubMed1975

Routine assay for detection of IgG and IgM antiglobulins in seronegative and seropositive rheumatoid arthritis.

A convenient technique suitable for the routine estimation of IgM and IgG antiglobulins has been devised. The assay involves the binding of antiglobulins (rheumatoid factors) to rabbit immunoglobulin linked to the surface of plastic tubes; the amount of antiglobulin bound is then determined by adding radiolabelled antihuman IgG or IgM. Both antiglobulins were raised in virtually all seropositive rheumatoid arthritics, and 19 out of 22 seronegative patients had raised values for either IgM or IgG rheumatoid factors. The test should prove valuable in diagnosis and the results further emphasize autosensitization to IgG as a dominant immunological characteristic of different forms of rheumatoid arthritis.

Animals↗
PubMed1975

Complement receptor lymphocytes. Analysis of immunoglobin on their surface and further evidence of heterogeneity.

Mouse complement receptor lymphocytes (CRL) were detected by a rosetting method. The existence of an immunoglobulin class or subclass on the central rosetting cell wasstudied by immunofluorescence using rabbit antisera specific for mouse IgM, IgG1, IgG2a, IgG2b and IgA. Combining immunofluorescence for each immunoglobulin and for T cells with cytoxic removal of T cells, CRL were demonstrated to include immunoglobulin-bearing cells of all the classes and subclasses tested (IgM being the main one), a 'null' cell subpopulation, and some T cells. A scheme describingthe various nature of CRL is discussed and correlated with possible functions.

Animals↗