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Biomedical subjects

F Boschi

Publications and source records attributed to F Boschi.

25 records · Page 2Linked to original sources

[Diaphanization. Testing method for endodontic technics].

It's a study about the principal clearing techniques, with a selection of materials and phases of execution. The purpose was to obtain transparency of mineralized structure together with preservation of pulpal contents. From this research a technique has been extracted and positively tested, with great results: a) tridimensional control of alterations pointed out in anatomy after canal preparation; b) measurement of pulpal debris size after instrumentation; c) identification of instruments dark zone; d) measurement of the empty-cleansing capacity of root canal system and the defence from eventual self-made errors, as a result of particular operating techniques, whose comparison is accessible by methodology.

Coloring Agents↗

Glycerol production in a triose phosphate isomerase deficient mutant of Saccharomyces cerevisiae.

Interesting challenges from metabolically engineered Saccharomyces cerevisiae cells arise from the opportunity to obtain yeast strains useful for the production of chemicals. In this paper, we show that engineered yeast cells deficient in the triose phosphate isomerase activity are able to produce glycerol without the use of steering agents. High yields of conversion of glucose into glycerol (80-90% of the theoretical yield) and productivity (1.5 g L-1 h-1) have been obtained by a bioconversion process carried out in a poor and clean medium. We obtained indications that the growth phase at which the biomass was collected affect the process. The best results were obtained using cells collected at the end of exponential phase of growth. In perspective, the strategies and the information about the physiology of the cells described here could be useful for the developing of new biotechnological processes for glycerol production, outflanking the problems related to the use of high level of steering agents.

Biotransformation↗

Hormonal changes in male subjects over-ninety.

The physiology of aging in very old people is still poorly understood. A wide variety of age-related factors confuse the interpretation of existing data, especially as regards the modifications of the endocrine system. The effect of age on the gonad-hypophyseal function was studied in 26 over ninety-year-old males and compared with a control aged between 27 and 88, subdivided into age groups. The serum levels of total testosterone were stable up to the ninth decade while they were lower in the over ninety-year-olds; free testosterone and its peripheral metabolites (estrogens and dehydrotestosterone) were also lower in the over ninety-year-olds with respect to the others, although the decrease had started more gradually at about the fourth decade. Sex-hormone-binding-globulin, the main serum carrier of testosterone, increased gradually with age. The levels of hypophyseal gonadotropins were constant up to the ninth decade where a significant and abrupt increase was observed. The data suggest the presence of marked alterations of the testicular-hypophyseal axis in the later decades of life, with important repercussions on the pattern of circulating sex steroids.

Adult↗