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Biomedical subjects

F Bonhomme

Publications and source records attributed to F Bonhomme.

At least 91 records · Page 5Linked to original sources
PubMed1985

Genes for skeletal muscle myosin heavy chains are clustered and are not located on the same mouse chromosome as a cardiac myosin heavy chain gene.

Myosin heavy chain (MHC) genes are expressed as several distinct isoforms in a tissue- and stage-specific manner; three skeletal muscle MHC isoforms appear sequentially during development. We have isolated cDNA clones, identified by RNA blot hybridization and by nucleotide sequence determination as coding for portions of the embryonic (pMHC2.2), perinatal (pMHC16.2A), and alpha(V1) cardiac (pMHC141 and pMHC101) MHC isoforms. These four probes and the adult skeletal MHC probe (pMHC32) have been used on Southern blots of genomic DNA to detect restriction fragment length polymorphisms defining the alleles for these genes in mouse species Mus musculus and Mus spretus. In this way, we followed the segregation of skeletal and cardiac MHC genes in 42 offspring resulting from an interspecies backcross. We found that the embryonic, perinatal, and adult skeletal MHC genes are clustered on chromosome 11 near the locus nude, the skeletal and cardiac MHC genes do not cosegregate, and the alpha(V1) cardiac MHC gene is located on chromosome 14 close to Np-1. This result is in contrast to that for other contractile protein genes such as the alkali myosin light chain and the actin multigene families, which are dispersed in the genome.

Animals↗
PubMed1985

Segregation of restriction fragment length polymorphism in an interspecies cross of laboratory and wild mice indicates tight linkage of the murine IFN-beta gene to the murine IFN-alpha genes.

Southern blot analysis with murine (Mu) interferon (IFN)-alpha cDNA of restricted genomic DNA of three inbred strains of mice belonging to the species Mus musculus domesticus (BALB/c, C57BL/6, and DBA/2) revealed only a limited degree of polymorphism. For example, with HindIII there were only two polymorphic bands out of 14 hybridizing fragments. With Mu IFN-beta cDNA there was no polymorphism at all between BALB/c and C57BL/6 in DNA restricted with seven different enzymes. In contrast, HindIII-restricted DNA of an inbred strain of wild mice (M. spretus Lataste) hybridized with the IFN-alpha probe displayed a high degree of polymorphism compared with the three strains of laboratory mice and was also polymorphic when probed with IFN-beta cDNA. Although M. musculus domesticus and M. spretus Lataste represent different species, certain interspecies crosses are possible in the laboratory. This enabled us to follow segregation of restriction fragment length polymorphism in HindIII-restricted DNA obtained from 18 backcross progeny of a (DBA/2 X M. spretus)F1 X DBA/2 interspecies cross. There was complete coincidence between the segregation of parental (DBA/2) and (DBA/2 X M. spretus)F1-type IFN-beta and IFN-alpha restriction fragment length polymorphism, indicating tight linkage of the IFN-beta and IFN-alpha genes. In addition, in 15 of 18 progeny the segregation coincided with that of the brown locus on chromosome 4, in accord with previous results obtained with the IFN-alpha probe in strains derived from crosses between BALB/c and C57BL/6 mice. Thus, the Mu IFN-beta gene is tightly linked to the Mu IFN-alpha gene cluster on chromosome 4 near the brown locus.

Animals↗
PubMed1984

Biochemical diversity and evolution in the genus Mus.

Thirteen biochemical groups of wild mice from Europe, Asia, and Africa belonging to the genus Mus are analyzed at 22-42 protein loci. Phylogenetic trees are proposed and patterns of biochemical evolution are discussed, as well as the possible contribution of wild mice to the genetic diversity of laboratory stocks.

Alleles↗
PubMed1984

Expression of GM1 and GD1a in liver of wild mice.

Wild mice are divided into two groups with different ganglioside compositions in the liver. Most Japanese and a few Chinese wild mice have GM2(NeuGc) as a major ganglioside, whereas all wild mice caught at other places distributed all over the world other than Japan and China express GM1(NeuGc) and GD1a(NeuGc) in addition to GM2(NeuGC). We recently reported that inbred strains of laboratory mice were also grouped into the same two types based on the ganglioside composition in the liver, and that the expression of GM1(NeuGc) and GD1a(NeuGc) was regulated by a gene located at the left outside the H-2 complex on chromosome 17 (Hashimoto, Y., Suzuki, A., Yamakawa, T., Miyashita, N., & Moriwaki, K. (1983) J. Biochem. 94, 2049-2054). The present study suggests that oriental wild mice would be a donor of a defective gene for expression of GM1(NeuGc) and GD1a(NeuGc) in mice of laboratory stocks which are commonly used for biochemical and immunological studies, such as C57BL/6, C57BL/10, BALB/c, DBA/2, C3H/He, and CBA mice.

Animals↗
PubMed1984

[Electrophoretic variability in a total of 11 structural sites in murine rodents (Muridae, Rodentia)].

Forty-six rodent species of the Muridae family were submitted to sequential electrophoresis for the study of 11 protein loci, using 12 buffer systems which differed in pH and ionic composition. The complete set of electrophoretic conditions yielded 135 variants of which 68 were detected through a single condition. The twofold increase of revealed variants was essentially limited to intergeneric comparisons because few additional variants within genera were revealed, despite the use of several buffers. These results show that estimation of the degree of genetic differentiation among taxa at a higher level than that of the genus by standard electrophoretic data may, although of current use, lead to erroneous results.

Animals↗
PubMed1983

Genetic polymorphism of lambda 1 and lambda 3 immunoglobulin light chains in the Mus subgenus.

Several inbred or partially inbred mouse strains derived from wild mice of different Mus subgroups were surveyed for expression of lambda 1 light chain. One strain, SPE, expressed little or no lambda 1 chains. Two populations of antibodies were obtained by immunization of SPE mice with lambda 1 light chain isolated from the BALB/c J558 (alpha, lambda 1) myeloma protein. One population of antibodies recognized lambda 1 allotypic determinants located on the C terminal fragment beginning at the residue 176. A second population was directed against epitopes present on both lambda 1 and lambda 3 chains. These determinants, which were detected in all strains tested with the exception of SPE, were located in the V region. This result is in concordance with recent DNA studies showing that the lambda 1 and lambda 3 isotype use the same V lambda 1 genes (Blomberg, B. et al., Proc. Natl. Acad. Sci. USA 1981. 78: 3765). Whether the V epitopes recognized by SPE antibodies are allotypic or isotypic in nature is not certain.

Animals↗
PubMed1982

[Presence of a male sterility factor, Hst-2, segregating in interspecies crosses. M. musculus L. x M. spretus Lastaste and linked to Mod-1, and Mpi-1 on chromosome 9].

F 1 hybrid males resulting from the interspecific cross Mus musculus L. X Mus spertus Lataste are sterile. The sterility depends on several independant genetic factors. Using a linkage analysis carried out with biochemical markers, we have been able to localize one of these designated Hst-2, close to Mod-1 on chromosome 9.

Animals↗
PubMed1982

Origins of laboratory mice deduced from restriction patterns of mitochondrial DNA.

To determine the origins of laboratory mice, the restriction patterns of mitochondrial DNAs (mtDNAs) from various strains were compared with those of relevant subspecies and/or races of Mus musculus. In most strains and substrains of laboratory mice examined (50/55), the cleavage patterns were identical to those of the European subspecies M. m. domesticus. Those that varied include two sublines of NZB, the strain NZC, and the Japanese strain RR. The NZB and NZC patterns were identical to that of the European subspecies M. m. brevirostris, which itself has restriction patterns similar to M. m. domesticus. On the other hand, the RR pattern was identical to M. m. molossinus-like mice trapped in Western China and slightly different from Japanese M. m. molossinus. These findings suggest that the strains NZB and NZC stemmed from a European founder stock which differed from the ancestral stocks of other laboratory strains and that the ancestral mice of the RR strain had been transported from China to Japan. Therefore, most laboratory strains of mice are derived from the European subspecies M. m. domesticus while M. m. brevirostris and M. m. molossinus have made minor contributions. M. m. musculus does not appear to have made any contribution.

Animals↗
PubMed1982

X-Y chromosome dissociation in wild derived Mus musculus subspecies, laboratory mice, and their F1 hybrids.

The frequencies of X and Y chromosome dissociation in primary spermatocytes of wild-caught specimens of six Mus musculus subspecies, fifteen inbred mouse strains, and their F1 hybrids were examined. Three different types (I, II, and III) of X-Y dissociation were identified. Type I was found in inter-subspecies hybrids (BALB/c X M. m. molossinus, M. m. bactrianus, M. m. castaneus, or M. m. urbanus), in which the X-Y dissociation was less than 20% in each subspecies but 60-80% in the f1 hybrids. Type II was seen in the DBA/2J and DDK strains, in which the dissociation frequency was about 30% in the homozygous condition and increased up to 50% in inter-strain hybrids (C57BL/6J X DBA/2J or DDK). Type III found in the WB/Re strain also showed high dissociation frequency (51.1%) in the homozygous condition but the value was remarkably lower (11.3%) in C57BL/6J X WB/Re)F1s. Some asian wild mice seem to have type II or III as well as type I.

Animals↗
PubMed1982

[Actiopathogenic study of per and postoperative haemorrhage in adenotonsillectomy in the child (author's transl)].

This study involved 4 201 records between July 1st 1969 and February 28th 1979. Various parameters represented by specific patient data (age, sex, ENT infections), laboratory examinations, operative findings and weather conditions were studied. Adenoidectomy was found to be more haemorrhagic than adenotonsillectomy, in particular in the presence of a large clump of adenoids and in autumn or winter. Use of a halothane-nitrous oxide-oxygen mixture decreased the number of haemorrhages which by contrast were increased by an operative position in a dorsal horizontal position. Finally, any deviation from standard meterological conditions would appear to be a pejorative factor. The authors conclude by stating that no serious complications were seen and that it is important to undertake a precise and complete preoperative laboratory assessment. Finally, mutual confidence between surgeon and anaesthetist is an additional factor in decreasing risk.

Adenoidectomy↗
PubMed1980

Histocompatibility-2 system in wild mice. X. Frequencies of H-2 and Ia antigens in wild mice from Europe and Africa.

In this study, data are presented on serologic H-2 typing of 320 wild mice collected in several parts of Europe and Egypt. The sample was typed for 39 class I (K, D) and 16 class II (Ia) antigens. The phenotype frequencies of class I and class II antigens showed high variability in their distribution among different areas, ranging from absence or presence in low frequency in one area to presence in about one-half of the mice in another area. The average phenotypic frequency of class I private antigens was 6.3%; at least 60% of class I alleles (blanks) could not be identified with the available reagents. The data suggest that there might be more than 100 alleles for each class I locus, H-2K and H-2D. The average phenotypic frequencies of Ia-1 private antigens was 10.8%. About 75% of Ia-1 alleles (blanks) could not be identified. The number of Ia-1 alleles was estimated to be in the range of 20 to 50.

Alleles↗
PubMed1979

[Genetic analysis of interspecific crosses Mus musculus L. x Mus spretus Lataste: linkage of Adh-1 with Amy-1 on chromosome 3 and Es-14 with Mod-1 on chromosome 9].

192 offsprings from interspecific back-crosses (male M. spretus x female BALB/c) F1 x male BALB/c or (male M. spretus x female C57BL6) F1 x male C57BL6 were analysed at thirteen structural loci. Linkage of ES-14 with Mod-1 on chromosome 9 and that of Adh-1 with Amy-1 on chromosome 3 are shown. The following order centromere/Car-2/Amy-1 is tentatively proposed for these loci on chromosome 3.

Alcohol Oxidoreductases↗