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Biomedical subjects

F Behrens

Publications and source records attributed to F Behrens.

At least 55 records · Page 3Linked to original sources
PubMed1981

Prototheca wickerhamii--an alga infecting the hand.

This report describes a hand infection caused by an alga. Prototheca wickerhamii. This rare organism presents most often as chronic dermatitis or bursitis of the upper extremity. Routine stains and cultures are easily confused with fungi. Only early, radical excision of the involved structures will effect a cure.

Adolescent↗
PubMed1981

Correlation of herpes simplex virus antibody titers and specific lymphocyte stimulation in adult blood donors.

Antibody titers to herpes simplex virus type 1 in sera from healthy adult donors were assayed by complement fixation, microneutralization, and an enzyme immunoassay (ELISA). This last test proved to be the most sensitive method for antibody detection. It was estimated that ELISA antibody titers were up to 40-fold higher than neutralizing antibody titers and up to 100-fold higher than complement fixation antibody titers. Due to the higher sensitivity of ELISA, only 3 of 36 blood donors tested in this assay were shown to be seronegative, whereas 6 additional persons of the same group were termed seronegative by the microneutralization assay. Furthermore, four of the latter also did not respond in the complement fixation test. In vitro stimulation of peripheral lymphocytes by using a partially purified herpes simplex virus type 1 particle antigen was achieved for all seropositive blood donors. Only those three donors who were ELISA negative reacted negatively in this stimulation assay. From these results it may be concluded that ELISA is an appropriate method not only for rapid and sensitive antibody determination but also for selecting herpes simplex virus-negative patients.

Adult↗
PubMed1980

The enzyme-linked immunosorbent assay (ELISA) for determination of IgG and IgM antibodies after infection with mumps virus.

Under routine laboratory conditions ELISA was tested for suitability for serological demonstration of specific antibodies of the immunoglobulin classes G and M against mumps virus. Sera from patients with known clinical and virological data were used. The results of ELISA were compared with those of CFT. 45 paired sera were tested in ELISA IgG, 87 first sera in ELISA IgM. Both tests were highly sensitive, antibodies were detected earlier and with higher titers than with the CFT. The ELISA IgM is particularly suitable for early diagnosis of mumps infection with the first serum. In addition 23 paired sera from patients with acute parainfluenza virus infection were examined for cross-reacting antibodies. Low anti-mumps IgG antibody titers were found in some sera. These findings reduced the mumps specificity of the IgG test. In five serum samples from one patient--obtained before, during, and after an infection with mumps--the course of IgG and IgM antibodies could be demonstrated. Advantages and limitations of ELISA IgG and IgM are summarized.

Antibody Formation↗
PubMed1979

Smooth pursuit eye movements and optokinetic nystagmus elicited by intermittently illuminated stationary patterns.

Stationary periodic visual patterns (row of equally spaced dots or black-white stripes) of the period Ps illuminated stroboscopically with a flash frequency fs induce an apparent movement perception (sigma-movement) when slow eye movements are performed across the periodic pattern. The movement appears in the direction of the eye movements when the angular speed VE of the eyes corresponds to the following condition: Ve = k . Ps . fs [deg . s-1] (1) k is a constant and equals 1 (or exceptionally 2 or 3). The sigma-movement induces a sigma-OKN with an average angular speed of its slow phases corresponding to Eq.(1). sigma-OKN can be elicited when identical foveal or identical extrafoveal stimulus patterns are applied from flash to flash. A considerable random variability of the flash sequence does not interrupt the sigma-movement and the sigma-OKN. Both phenomena can also be elicited by a stimulus pattern with its periodicity hidden in spatial noise and this periodic pattern only becomes visible during the eye movements. It is argued that the sigma-phenomena are caused by efference copy signals of the gaze control system, which interact with the afferent signals (displacement of visual stimuli on the retina) at different levels of the afferent visual system. One interaction is supposed at a cortical level where the extrapersonal visual space is represented.

Adult↗
PubMed1978

Diarrhea in newborn cynomologus monkeys infected with human rotavirus.

Of six newborn cynomolgus monkeys (Macaca fascicularis) naturally delivered and normally nursed five developed diarrhea after oral administration of human rotavirus. Virus excretion was observed in the stool of four animals. This virus was transmitted to four out of six other monkeys causing diarrhea in only one animal.

Animals↗
PubMed1977

[Seroepidemiological Investigations on the epidemiology of human rotavirus infections (author's transl)].

263 sera of infants and children up to 10 years of age were tested for antibodies (CF-test) against Nebraska calf diarrhoea virus (NCDV) which is antigenically related to the rotavirus of man; in addition the mean antibody titers in different age groups were investigated. Antibodies of maternal origin were eliminated during the first year of life. Most of the infections occurred during the second year of life and at the end of this year 40-50% of the investigated children demonstrated antibodies against NCDV; this frequency persists during the rest of the investigated life period.

Antibodies, Viral↗
PubMed1976

Metabolic recovery of articular cartilage after intra-articular injections of glucocorticoid.

The recovery of adult rabbit articular cartilage after nine weekly intra-articular injections of hydrocortisone acetate was investigated using biochemical, autoradiographic, and tracer methods. After the cessation of steroid injections, the rates of synthesis of proteoglycans and collagen increased by up to 900 per cent. The increase occurred because of accelerated metabolism of existing cells and an increase (caused by mitosis) in the number of functioning chondrocytes. The proteoglycan concentration only returned to normal values after six months.

Animals↗
PubMed1975

Alterations of rabbit articular cartilage by intra-articular injections of glucocorticoids.

The destruction of rabbit articular cartilage after intra-articular injections of hydrocortisone acetate was investigated using histological, biochemical, and tracer methods. Fissures and cysts increased in number as increasing amounts of hydrocortisone were given. A linear decrease of hexosamine to less than 50 per cent after twelve injections was accompanied by insigificant changes in deoxyribonucleic acid and hydroxyproline content. The synthesis of proteoglycans and proteins was reduced to one-third, while the production of collagen dropped to less than one-fifth. The changes in thymidine incorporation were not significant. Based on these data, a model indicating the sequence of events which leads to joint destruction after intra-articular injections of glucocorticoid is proposed.

Animals↗
PubMedDate not supplied

An automated and modified technique for testing the retinal function (Arden test) by use of the electro-oculogram (EOG) for clinical and research use.

The electro-oculogram (EOG) can be recorded by use of skin electrodes positioned near the eye in the horizontal or vertical plane and is dependent on the position of the eye relative to the electrodes and the size of the corneo-retinal potential (CRP). By use of the functional relation between the recorded potential and the eye position - CRP is considered to be constant - the EOG is the eye movement recording method most often used. Changes in CRP dependent on luminance or metabolic influences can be measured, on the other hand, by means of the EOG when eye movements of constant amplitudes are performed. The EOG in this paper is used in the latter sense. The widely used clinical test of rentinal function based on the electro-oculogram firstly described by Arden (Arden index) has been modified to achieve more precise and reliable results by including a gradual dark adaptation procedure and a special data analysis to detect fixation periods. Using modern techniques, the complete procedure has been automated. The efficiency of the procedure and the device is demonstrated in various ways.

Automation↗