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Biomedical subjects

F Bartos

Publications and source records attributed to F Bartos.

At least 55 records · Page 3Linked to original sources
PubMed1977

Stereospecific antibodies to methadone. I. Radioimmunoassay of d,l-methadone in human serum.

Anti-d,l-methadone antibodies were produced in rabbits immunized with d,l-methadol-hemisuccinate thyroglobulin conjugate. Using the antiserum, a radioimmunoasay (RIA) for determination of d,l-methadone in human serum has been developed and is described. Concentration of d,l-methadone of 1.4 pmol in a native serum sample (volume 0.1 ml or less) could be measured directly by RIA. The antibodies crossreact 100% with d,l-methadone, 50% with d-methadone, 50% with l-methadone and 100% with alpha-d-methadol. No crossreactivity was found with alpha 1-methadol, morphine, meperidine, dextropropoxyphene, 2-ethyl-5-methyl-3,3-diphenyl-l-pyrroline and 2-ethylidene-l, 5-dimethyl-3,3-diphenylpyrrolidene. High sensitivity and small sample requirements make this method suitable for future monitoring of patients on methadone maintenance and for studies where other procedures have lack of sensitivity.

Animals↗
PubMed1977

Inhibitory effect of adrenaline and hydrocortisone on the growth of Allium cepa roots.

The roots of Allium cepa were allowed to grow in distilled water containing 10(-4) M adrenaline hydrochloride or 2 X 10(-3) M hydrocortisone sodium succinate. Adrenaline inhibited the growth of roots; they decreased in length, number and total dry weight. The total amount of DNA in the roots was reduced much less than that of extracellular root components after adrenaline. Also hydrocortisone treatment resulted in a considerable decrease of the length and dry weight of Allium cepa roots. Both DNA and extracellular root components were influenced.

Cell Division↗
PubMed1975

Normal functions of the thyroid gland of the pygmy goat.

Normal values were obtained for blood serum PBI, T3 index. T4, and cholesterol in a colony of pygmy goats (n equal to 55) of mixed sex and age. Serum PBI values averaged 8.1 plus or minus 1.2 mug/dl with no significant sex differences. The mean T3 index and T4 value were 1.1 plus or minus 0.1 and 7.2 plus or minus 1.1 mug/dl, respectively, with no sex differences. The mean serum cholesterol value was 90.0 mg/dl, with sex differences apparent. Serum cholesterol averaged 84.9 mg/dl (n equal to 44) for females and was significantly higher in intact males (97.4 mg/dl; n=8) and significantly lower in castrate males 69.2; n=6. There was a consistent and significant increase in cholesterol values with age in females, an unexplained phenomenon also observed in humans. There was no evidence of thyroid malfunction in the animals studied.

Age Factors↗
PubMed1975

Direct determination of polyamines in human serum by radioimmunoassay.

Antipolyamine antibodies were produced following immunization of New Zealand white rabbits with thyroglobulin spermine conjugate, using multiple-site injection and macrophage-harvesting techniques. The antispermine antibody in a radioimmunoassay system cross-reacted with spermidine (22%), putrescine (1%), diaminopropane (6%), and cadaverine (0.16%). No reaction with L-lysine, ornithine, or histamine was noted. Using this antibody, the radioimmunoassay for direct determination of serum polyamines was developed. A sensitivity of 200 pg was achieved on human serum samples. This radioimmunoassay would appear to be a useful tool for the detection and monitoring of a variety of pathophysiological states associated with abnormal polyamine metabolism, i.e., cancer.

Animals↗