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Biomedical subjects

F Barin

Publications and source records attributed to F Barin.

At least 145 records · Page 8Linked to original sources
PubMed1983

[Detection of IgM anti-hepatitis A antibodies by immunoenzyme technic. Interpretation of results and comparison of 2 tests (Havab-M EIA and Hepanostika anti-HAV IgM].

The presence of IgM anti-HAV was investigated in 60 patients with acute hepatitis A using two commercialy available enzyme linked immunosorbent assays (Havab-M EIA, Abbott, and Hepanostika anti-HAV IgM, Organon). High levels of anti-HAV IgM were present for only 30 to 60 days after the onset. Low levels of anti-HAV IgM were detected for as long as two years after acute phase. These results suggest that only high levels of anti-HAV IgM have to be considered for the diagnosis of a recent infection by hepatitis A virus.

Adolescent↗
PubMed1982

Immune response in neonates to hepatitis B vaccine.

Three injections of alum hepatitis B vaccine (HB vaccine) were given to 26 Senegalese infants, the first when they were less than 1 month old, and the second and third after 1-month intervals. 94.7% of the neonates whose serum was negative for hepatitis B surface antigen and antibodies against the surface antigen (anti-HBs) showed a specific anti-HBs response. Anti-HBs titres in the neonates were similar to those previously obtained in Senegalese children aged aged 1-24 months. HB vaccine was without sid-effects and was immunogenic in the neonates, irrespective of the hepatitis-B-marker status of the mothers or the children.

Antibodies, Viral↗
PubMed1982

[Hepatitis B virus and hepatoma: evidence for a cytoplasmic tumor antigen in transformed hepatocytes].

A tumoural antigen (HBV/T Ag) has been found in the cytoplasm of the human hepatoma cell line PLC/PRF/5 by indirect immunofluorescence and immunoperoxidase assays using anti-sera from Senegalese patients with primary hepatocellular carcinoma. The same antigen was detected in PHC tissues. According to the data obtained with serum and tissue controls the HBV/T Ag-anti-HBV/T system seems associated with transformation of hepatocytes by hepatitis B virus.

Antigens, Viral↗
PubMed1982

Large-scale isolation of the two major polypeptides of the hepatitis B surface antigen (HBsAg) by gel filtration in 6 M guanidinium chloride.

Purified hepatitis B surface antigen (HBsAg) of subtype ay was solubilized in guanidinium chloride and submitted to chromatography on Sepharose 4B in the presence of guanidinium chloride. The polypeptides P1 (Mr = 24,000) and P2 (Mr = 29,000) were eluted in the same fraction with a minor contaminant (Mr = 40,000). Large amounts of these two polypeptides were obtained in a single step. This technique which constitutes a method for large-scale purification of the P1 and P2 polypeptides should permit more complete characterization of the P1 and P2 polypeptides and of their antigenic determinants.

Chromatography, Gel↗
PubMed1982

[Vaccination against hepatitis B in a hemodialysis unit. A 4-year follow up (author's transl)].

From February 1976 to January 1980, 123 staff members and 84 hemodialysis patients were immunized against hepatitis B in an adult hemodialysis unit. The vaccine was prepared by purification of HBs Ag from human sera and was formalin inactivated. Vaccines were tested for seric markers of HB virus (HBs Ag, anti-HBs, anti-HBc) and serum transaminases (ALT, AST) before immunization and every month during the follow up. Tests for markers of auto-immunity were performed. The vaccinees were followed from 4 to 48 months. No evidence of long-lasting reactions to the HB vaccine or auto-immunity was observed. 91% of the staff members sero-converted for anti-HBs; none of them showed clinical, biologic or serologic signs of active HB infection; 62% of the hemodialysis patients sero-converted for anti-HBs; none of them became HBs Ag chronic carrier. These results were obtained despite the fact that the prevalence of HBs Ag chronic carriers was 32.7% when the study began. Active immunization proved to be a safer and efficient method to prevent HB infection in a hemodialysis unit.

Autoantibodies↗
PubMed1981

Efficacy of hepatitis B vaccine in prevention of early HBsAg carrier state in children. Controlled trial in an endemic area (Senegal).

Three doses of inactivated hepatitis B vaccine were given at one-month intervals to Senegalese children aged less than two years. A control group received diphtheria/tetanus/polio vaccine. Of those HB vaccine recipients who were seronegative before immunisation, 94.5% had a specific anti-HBs response. Anti-HBs of maternal origin did not interfere with the active immunisation. HB vaccine was without ill-effects, irrespective of hepatitis B marker status before immunisation. After twelve months' follow-up, the incidence of the HBsAg carrier state was reduced by 85% in susceptible children (p less than 0.0001).

Age Factors↗
PubMed1978

Large scale purification of hepatitis B surface antigen (HBsAg).

In October 1975, a specific immunization by means of a formalin inactivated hepatitis B vaccine has been introduced to protect patients and staff members of three haemodialysis units of the Loire Valley (Tours, Blois, Orléans). After two years follow-up the innocuity and efficacy of this preparation have been shown to be very satisfactory in the conditions under which it was used. A method of vaccine preparation has been instituted in the development of industrial batches of vaccine to be used for broad clinical trials in France. The HBsAg purification was carried out by four different steps including, successively, selective adsorption-desorption on colloidal silicate (Aerosil), precipitations by polyethylene glycol, gel filtration and finally zonal ultracentrifugation. Step-by-step results of the purification are presented. Up to 65 % of the starting antigen was recovered at the end of the purification process. One dose of vaccine (1 ml) has a titre in HBsAg of 1/4 in countercurrent electrophoresis and a protein amount of 2-10 micron/ml. It contains traces of homologous serum proteins only detectable after high concentration. Purity, antigenic quality and safety of the vaccine are analysed in regards to its use for immunization against hepatitis B in man.

Centrifugation, Zonal↗
PubMedDate not supplied

Brain membrane disordering related to acute ethanol administration in naive and short-term ethanol-intoxicated rats.

Crude synaptic membrane fluidity (checked by fluorescence polarization) together with (Na+ + K+) ATPase activity were examined 18 hours after a single oral ethanol administration (5 g/kg bwt.) to naive rats and to rats previously intubated with ethanol repeatedly during 4 days. The sensibility of both parameters to different concentrations of ethanol added in vitro (0.175 M-1.400 M) was also determined. Although no changes in the basal intrinsic fluidity were found, (Na+ + K+)ATPase activity increased slightly in both conditions. The fluidizing as well as the ATPase inhibiting effects following the addition of ethanol in vitro were markedly increased 18 hours after ethanol administration to naive rats. This hypersensitization was no longer apparent in rats pretreated with ethanol during 4 days. The acute ethanol-induced hypersensitization found in naive rats appears not to be related to an unspecific stress or to changes in body temperature. The disappearance of this hypersensitization in short-term alcohol-intoxicated animals may represent the first stage of tolerance acquisition.

Alcoholic Intoxication↗