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F B Gelder

Publications and source records attributed to F B Gelder.

33 records · Page 2Linked to original sources
PubMed1986

Purification, partial characterization, and clinical evaluation of an adenocarcinoma-associated antigen.

The current investigation describes the purification and partial characterization of a new adenocarcinoma-associated antigen (ACAA). ACAA is a large molecular weight glycoprotein (Mr 790,000 by size chromatography on Sepharose CL-6B) that migrates in the alpha 1 region upon electrophoresis and is eluted from a DEAE-cellulose column at a 0.1 M NaCl concentration. ACAA is immunochemically and biochemically different from carcinoembryonic antigen, alpha-fetoprotein, pancreatic oncofetal antigen, human pancreatic tissue antigen, CA 19-9, ferritin, and acute-phase proteins. Assays for ACAA were carried out using a solid-phase sandwich enzyme immunoassay. The results indicate that ACAA is present in sera of all individuals. Patients with cancer have higher serum levels of ACAA than normal individuals. The greatest frequency of elevated serum values of ACAA was seen in patients with lung and pancreatic cancers followed by colorectal, breast, and prostate cancer. The measurement of ACAA levels may be valuable in the diagnosis and clinical management of patients with certain cancers.

Adenocarcinoma↗
PubMed1985

Pancreatic oncofetal antigen in the detection of experimental cancer of the pancreas.

A hamster serum protein which reacted with anti-human pancreatic oncofetal antigen (POA) was identified in sera from hamsters with varying types of pancreatic pathology. Hamsters were treated with N-nitrobis-(2-hydroxypropyl)-amine, and histological findings in the pancreas were correlated with hamster POA. The cross reactivity of anti-human POA with hamster POA was poor. As a result, the sensitivity of this assay was too low to permit its use in serologically discriminating carcinogen-induced pancreatic cancer from other pancreatic pathologies. A specific antiserum and assay system to hamster POA may overcome this problem.

Animals↗
PubMed1985

Development and evaluation of a new kinetic assay for the quantitation of fibronectin gelatin-binding activity.

A new rapid and sensitive kinetic assay that measures the gelatin-binding activity of fibronectin has been developed. This assay is based on the rate of fibronectin-mediated aggregation of covalently coupled latex-gelatin particles. The addition of human plasma and serum resulted in aggregation rates proportional to the concentration of fibronectin in the test sample. This assay was inhibited by the addition of gelatin, demonstrating substrate specificity. This new assay requires 50 microliter of sample and can be performed within 5 minutes. Particle aggregation rates were affected by pH, heparin, and coupled gelatin concentration per milligram of latex. Maximum aggregation rates were observed at pH 8.0. Heparin was not an absolute requirement for particle aggregation but enhanced rates up to 1 U heparin/ml with little additive effect at greater concentrations. Heparin had a relatively greater effect on assays performed in acidic buffers. The concentration of gelatin per milligram of latex was rate limiting up to approximately 50 micrograms gelatin/mg latex with little change in aggregation rates at higher concentrations. Good correlation between total antigenic fibronectin (electroimmunoassay) and fibronectin gelatin-binding activity (latex-gelatin kinetic aggregation assay) was demonstrated in plasma from normal blood donors. This new assay will allow further definition of the relationship between fibronectin gelatin-binding activity and antigenic fibronectin in normal and pathophysiologic states.

Electrophoresis, Polyacrylamide Gel↗
PubMed1983

Fibronectin and anchorage-independent and anchorage-dependent growth of benign and malignant cell lines.

The presence of fibronectin in three "malignant" (AU-471, AU-436, LT-2) and two "benign" (BHK-21, WI-38) cell lines was demonstrated with a fluorescent antibody technique; two malignant (AU-471, AU-436) cell lines were fibronectin-negative and one (LT-2) retained fibronectin expression. One "benign" cell line (WI-38) expressed fibronectin, the other (BHK-21) did not. Anchorage-independent soft agar (AISA) growth correlated better with loss of fibronectin than with malignant potential. All three fibronectin-negative cell lines (benign and malignant) grew anchorage-independently (AU-471, AU-436, BHK-21), and both fibronectin-positive cell lines were anchorage-dependent (LT-2, WI-38). Surprisingly, the addition of Clg to anchorage-independent cells increased their anchorage-independent soft-agar cloning efficiency, but had no effect on anchorage-dependent cell lines. Anti-Clg antibodies decreased AISA growth. The effect of Clg on anchorage-independent growth varied with the concentration, and also between cell lines, and a variation in effect was noted between anchorage-independent (AISA) and anchorage-dependent (in flasks) growth even in the same cell line.

Cell Line↗
PubMed1982

Malignant lymphoma occurring in a family.

Three patients, a 57-year-old man, his 60-year-old sister, and 81-year-old mother, developed non-Hodgkin's lymphoma within an interval of 12 months. There were histologic similarities of the biopsy material from these patients, but the clinical response was variable. Two patients achieved a sustained remission, and one expired after similar chemotherapy. Studies revealed slightly decreased numbers of circulating T lymphocytes in both surviving patients, and decreased cellular reactivity to mitogens in the man. HLA typing was not conclusive; HLA haplotypes were not the same as those reported in other families with non-Hodgkin's lymphoma. Electron microscopy of biopsy material revealed no viral inclusions. Except for the familial relationship, no common etiologic factors were identified.

Aged↗
PubMed1982

A heterophile system in human renal transplantation. X. HTA sensitivity includes sensitivity to human B lymphocytes.

Antibodies that react with heterophile transplantation antigen (HTA) have been shown previously not to react with HLA-A, B, or C antigens. This paper presents evidence that anti-HTA does react with a subpopulation of human lymphocytes which is comprised primarily of B cells. Anti-HTA reactivity was removed from sera by absorption with each of three different human B lymphocyte cell lines, but it was unaffected by absorption with platelets or thymocytes. Selected high titer anti-HTA sera absorbed with human platelets, human blood group type AB erythrocytes, and sheep erythrocytes caused lysis of a lymphocyte subpopulation principally composed of B lymphocytes. Absorption of these sera with rat erythrocytes removed both lymphocytic activity and anti-HTA activity. Antibody recovered by affinity purification with rat erythrocyte membrane preparations contained both lymphocytic and anti-HTA reactivity. These data, considered with previous studies, seem to establish that B cell sensitization may be acquired by a substantial segment of the population by natural immunization from enteric flora and/or by infections with enteric bacteria.

Animals↗
PubMed1982

A human myeloma cell line that does not express immunoglobulin but yields a high frequency of antibody-secreting hybridomas.

We selected an 8-azaguanine-resistant variant of a human myeloma cell line (RPMI 8226) by cloning the parental cells on a feeder layer of mouse spleen cells in the presence of increasing concentrations of 8-azaguanine. Culture media and cellfree extracts of both the parental and variant (8226 AR/NIP4-1) cell lines were assayed for production of immunoglobulin heavy and light chains by double immunodiffusion and for lambda-chain by radioimmunoassay. Secretion of free lambda-chain by the parental cell line was confirmed. In contrast, no immunoglobulin heavy or light chains were detected in culture medium of the variant cell line by either immunodiffusion or radioimmunoassay. No intracellular lambda-chain could be detected in the variant cells by radioimmunoassay of cellfree extracts or by immunofluorescence of fixed cells. Hybridomas were produced by fusion of 8226AR/NIP4-1 cells with lymphocytes from a mesenteric lymph node recovered at surgery from a hypertransfused renal transplant recipient. Twenty hybrid culture supernatants were assayed for immunoglobulin by double immunodiffusion, and 15 contained either IgG (lambda) or IgG (kappa). None produced IgM or IgA. An IgG (kappa)-producing hybridoma was shown by immunofluorescence not to express lambda-chain. A second fusion between the variant cell line and spleen cells from a renal transplant patient produced a stable hybridoma secreting IgM (lambda) antibody specific for the I antigen.

Animals↗
PubMed1982

Development of a transplantable model of pancreatic duct adenocarcinoma.

We report here the first development of a continuous cell line in tissue culture of an animal pancreatic duct adenocarcinoma that is histologically similar to human pancreatic duct adenocarcinoma. A primary pancreatic duct adenocarcinoma, induced in a male Syrian golden hamster after 23 weeks of weekly subcutaneous injection of N-nitrobis (2-hydroxypropyl)amine, was minced and injected subcutaneously into three hamsters. After 8 weeks, a single tumor was apparent. Subsequent passages of fragments into the cheek pouches were performed at 3- to 4-week intervals. After five passages, minced fragments of a tumor were placed in tissue culture. Colonies appeared by 7 days; an epitheloid cell line, without fibroblasts, was established by 60 days. Single-cell suspensions, injected into hamster cheek pouches or subcutaneously, produced tumors in a dose-dependent fashion. Spent culture medium of tissue culture cells and saline extracts of freshly excised tumors contained pancreatic oncofetal antigen-like activity.

Adenocarcinoma↗
PubMed1980

A heterophile system in human renal transplantation. VIII. The morphological distribution of the antigen in rat tissues.

The morphological distribution of heterophile transplant antigen (HTA) was determined in rat tissues using an indirect immunofluorescence technique. Human anti-HTA sera were used to localize HTA in rat kidney, liver, heart, skeletal muscle, spleen, and stomach. HTA was found in basement membrane and supporting stromal elements of all tissues studied. In the kidney, HTA was demonstrated in tubular basement membrane but not glomerular basement membrane. No evidence for cell surface antigen distribution could be ascertained except for erythrocyte membranes. HTA was not found on endothelium of rat blood vessels. We know of no antigens previously implicated in histocompatibility that are stromal in location.

Animals↗
PubMed1980

A heterophile system in human renal transplantation. IX. Comparison of heterophile transplantation antibodies to antivascular endothelial cell antibodies.

Antibodies to heterophile transplant antigen (HTA) were tested for reactivity with antigens on human umbilical cord antigenic specificities on isolated endothelial cells. Furthermore, there are antigens on endothelial cells that are distinct from HLA-A,B, C, and from HTA. It is concluded that the HTA and the VEC antigens are different.

Animals↗
PubMed1980

Surface distribution of monosialoganglioside GM1 on human blood cells and the effect of exogenous GM1 and neuraminidase on cholera toxin surface labeling. A quantitative immunocytochemical study.

The cholera toxin-colloidal gold-labeled IgG-F(ab')2 anticholera toxin ultrastructural immunocytochemical procedure was used for the localization of GM1 monosialoganglioside on the surface of human blood cells. The number of gold particles per micron of cell surface was counted and the data subjected to statistical analysis. Cholera toxin (CT) binding characteristics assessed in several subjects showed consistent labeling patterns for the various hemic cells, although some quantitative differences were noted in surface labeling densities between subjects. Neutrophils were invariably the most heavily labeled of the hemic cells, while lymphocytes, erythrocytes, and platelets exhibited only limited CT labeling. Exposure of hemic cells to neuraminidase induced a major increase in surface CT labeling that proved to be directly related to cell type and differed in many respects with the CT labeling pattern noted in nonenzyme treated cells. Newly exposed CT binding sites attributed to "masked" GM1 and/or to neuraminidase-transformed GD1a or GT1 gangliosides, showed that the number of new binding sites were nearly twice as abundant on platelet and monocyte sufaces as on the surfaces of neutrophil, lymphocyte, and erythrocyte populations. However, ratios of new CT binding sites to those normally available for CT binding were approximately 10:1 for erythrocytes, approximately 3--7:1 for lymphocytes, monocytes, and platelets, and approximately 1:1 for the neutrophil group. Exogenous GM1 was incorporated into the cell surface of the hemic cells in a differential manner. Platelets showed a dramatic increase in surface CT labeling, viz. approximately 12- to 20-fold, compared to that of other hemic cells; however, neutrophil and erythrocyte GM1 uptake was limited. Our studies have demonstrated that distinct differences exist in the extent of surface CT labeling of the various types of blood cells. They further indicated that the ability of the cell surface to incorporate exogenous GM1 may represent a differential expression of the physiochemical properties of the surface of the individual cell types.

Adult↗
PubMed1980

Differential expression of surface monosialoganglioside GM1 in various hemic cell lines of normal human bone marrow. A quantitative immunocytochemical study using the cholera toxin-gold-labeled anti-cholera toxin procedure.

The cholera toxin-colloidal gold-labeled IgG-F(ab')2 anti-cholera toxin ultrastructural immunocytochemical procedure has been used for the localization of GM1 monosialogangliosides on the surface of human bone marrow cells. The number of gold particles per micron of cell surface was counted and the data subjected to statistical analysis. Cholera toxin (CT) binding characteristics assessed in several subjects showed consistent labeling patterns for the various types of marrow cells, although minor quantitative differences were noted in surface labeling densities between subjects. Surface labeling was nonuniformly distributed along the cell membrane of the marrow cells and label clusters or domains were commonly noted. Data analysis indicated that CT labeling was related to cell type, to cell lineage, and to the stage of maturation. Mature neutrophils were the most reactive of the marrow cells and the CT labeling of this cell series increased stepwise from the promyelocyte stage to the segmented neutrophil. A similar pattern occurred during eosinophil maturation and the maturation of the monocyte. A different labeling pattern was found during the differentiation of the erythrocytic cell series with low labeling of proerythroblasts increasing modestly to the early normoblast stage and then decreasing during the final phase of maturation. Exposure to neuraminidase prior to the immunocytochemical sequence induced a major increase in surface CT labeling of the various types of marrow cells, as was particularly evident for the platelet, promyelocyte, myelocyte, monocyte, promonocyte, and erythrocyte cell groups. The data indicated that the number of cryptic GM1 and/or higher gangliosides exposed by neuraminidase in the cell membrane varied during cell differentiation and was directly related to specific cell types. Exogenous GM1 also was demonstrated to be incorporated into the surface of the bone marrow cells in a differential manner and the extent of incorporation was found to be related to specific cell types and to their stage of maturation.

Antitoxins↗
PubMedDate not supplied

Comparison of the immunogenicity of hepatitis B vaccine administered intradermally and intramuscularly.

Although hepatitis B vaccine reliably induces immunity to hepatitis B virus, the expense of intramuscular (im) vaccination with this product has limited its use. To determine if a smaller, less expensive, intradermal (id) dose of hepatitis B vaccine would be an effective alternative, we compared the response of antibody to hepatitis B surface antigen (anti-HBs) following im vaccination to that following id vaccination. Volunteers who were seronegative for antibody to hepatitis B core antigen were enrolled in the study and received either im or id vaccine. A total of 108 subjects received three 1-mL im injections of hepatitis B vaccine, and another 110 subjects received four 0.1-mL id injections of the vaccine. Similar rates of seroconversion occurred; greater than or equal to 10 mIU of anti-HBs/mL was noted following either three im or three id vaccinations. Furthermore, 2 years after initiation of vaccination, the serum concentration of anti-HBs for id vaccine recipients was similar to that for im vaccine recipients.

Adult↗