Search PubMed⌕ Search

Biomedical subjects

F Amenta

Publications and source records attributed to F Amenta.

At least 289 records · Page 16Linked to original sources

Histochemical studies on the autonomic innervation of the femoral artery and vein.

The innervation of the femoral artery and vein in the dog was studied using: a) glyoxylic acid fluorescence (for the histochemical localization of adrenergic nerve fibers); b) acetylcholinesterase (for the histochemical localization of cholinergic nerve fibers). -Andrenergic nerve fibers and related terminals in both vessels are confined to the adventitial-medial transitional zone and the outer layers of the media. Acetylcholinesterase containing nerve fibers are localized in the adventitial-medial transitional zone of the femoral artery. These findings may indicate the presence of a cholinergic vasodilatator system in the control of the circulation in the femoral artery.

Acetylcholinesterase↗

Cholinergic nerves in blood vessels of the female reproductive system.

Cholinergic innervation of the blood vessels of the female reproductive system was investigated in human by the cholinesterase method. The following results were obtained: 1. The uterine and vaginal arteries are provided with a rich cholinergic innervation. 2. The ovarian, tubaric and ovarian-uterine artery are poorly innervated. 3. The ovarian and uterine vein are not provided with a cholinergic innervation.

Acetylcholinesterase↗

Tests to localize free proteolytic enzymes in vivo by 14C-cysteine-aprotinin.

Aprotinin, a polyvalent protease inhibitor from bovine organs, has been labelled with 14C-cysteine in 6 M urea to produce a radioactive conjugate, without effect on the inhibitor activity. 138x10(3) dpm of radioactive product, containing 5 mg of protein (20.000 kal. inhibitor units) in 5 ml of Locke's solution were perfused in anesthetized rabbits via ascending aorta. Then the anesthetized rabbit was killed and specimens of some organs were admitted to autoradiographic analysis. Small intestine, ischiatic nerve and testis have been the most rich radiolabelled organs (+++). In kidney, stomach, large intestine, liver and eye the radiolabelled aprotinin was found in fair amounts (++); while in pancreas, spleen, spinal cord, brain, cava vein and aorta of rabbit the 14C-cysteine-Aprotinin was practically undetectable by autoradiography.

Animals↗

Cholinergic nerves in the thyroid gland.

The cholinergic innervation of the thyroid gland has been studied in the human. AChE positive nerve fibers are localized in the wall of the thyroid artery, arranged in two plexuses, a superficial (adventitial) and a deep one (medial). The glandular tissue is provided with cholinergic nerve fibers, localized between and around thyroid follicles. The present results suggest that the endocrine activity of the thyroid gland is also under the control of the autonomic nervous system.

Acetylcholinesterase↗

[Adrenergic receptors in coronary arteries (author's transl)].

The relations between adrenergic nerve fibers and related receptors in the wall of the coronary arteries have been studied in man. On sections of these vessels morphological observations (H.E.) were carried out: histochemical staining of adrenergic mediators (Eranko reaction for norepinephrine, and Hillarp reaction for cathecolamines), and of beta-receptors by means of a beta-blocking fluorescent drug (pindolol-Visken LB 46). Our observations provide evidence of the presence of beta-receptors in the wall of the coronary arteries, but they are particularly concentrated in the periadventitial connective tissue, in the adventitia and in the intima-media transitional zone. Beta-adrenergic receptors are above all present in the most richly innervated part of the vascular wall, and are therefore in close rapport with adrenergic nerve fibers.

Adolescent↗

(3H)-spiroperidol binding sites in the rabbit splenic artery.

The topographic distribution of (3H)-spiroperidol binding sites (corresponding to dopamine receptors likely) was studied in rabbit splenic artery by the use of a histoautoradiographic technique. (3H)-spiroperidol was localized preferentially at the level of smooth muscle cells of the media as well as in close relation with cellular membrane of endothelial cells. The findings of a constant distribution of (3H)-spiroperidol binding sites within the medial layer of the splenic artery led us to hypothesize that these receptors mediate the relaxation of the splenic artery determined by dopamine infusion.

Animals↗