Binding of 14C-phosphatidylcholine to sodium chloride crystals.
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Biomedical subjects
Publications and source records attributed to F A Green.
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Lecithin-(14)C was found to bind to Pyrex glass. The percentage bound varied with different organic solvents, being greatest in dioxane solution. Thorough treatment of the glass with dimethyldichlorosilane prevents the binding, which suggests that charge-charge interactions are involved.
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Acetylation of erythrocytic membranes by aspirin has not been reported. When erythrocytes or erythrocytic membranes are incubated with [acetyl-14C]aspirin, there is good evidence of very tight binding to membrane peptides in concentrations known to occur in vivo, 20 to 200 microM. No such evidence is observed with [carboxy-14C] aspirin. This indicates acetylation of membrane peptides. Although pronounced selectivity is not observed among the different peptides by gel filtration on Bio-Gel A-5M and sodium dodecyl sulfate polyacrylamide gel electrophoresis, there is overall saturation with a half-maximum at about 10 mM. The clinical significance of acetylation of membrane peptides of erythrocytes is unclear.
A 46-year-old man with acute gout and a dissected popliteal cyst presented with clinical features which mimicked rheumatoid arthritis and thrombophlebitis. The clinical and laboratory diagnosis of the case are presented and similar cases previously reported are briefly reviewed.
A forty-six year old man with acute gout and a dissected popliteal cyst presented with clinical features which mimicked rheumatoid arthritis and thrombophlebitis. The clinical and laboratory diagnosis of the case are presented and similar cases previously reported are briefly reviewed.
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Knees, hands, forearms and thighs in 22 patients with classical rheumatoid arthritis and 18 controls were imaged with 99mTc pyrophosphate. The uptake of 99Tc pyrophosphate in the joints of the hand and knee was quantitated using a gamma camera interfaced to a computer. Quantitative measurements of joint activity were assessed by analyzing resultant data in a number of ways. Counts per unit area normalized for body weight and dose of radiopharmaceutical was found to be the most satisfactory method of quantitating differences in disease activity. However, the sensitivity of the method was not great enough to clearly distinguish normal and rheumatoid joints.