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Biomedical subjects

Emad S Ebbini

Publications and source records attributed to Emad S Ebbini.

5 recordsLinked to original sources

Phase-coupled two-dimensional speckle tracking algorithm.

A new two-dimensional (2-D) speckle tracking method for displacement estimation based on the gradients of the magnitude and phase of 2-D complex correlation in a search region is presented. The novelty of this approach is that it couples the phase and magnitude gradients near the correlation peak to determine its coordinates with sub-sample accuracy in both axial and lateral directions. This is achieved with a minimum level of lateral interpolation determined from the angles between the magnitude and phase gradient vectors on the sampled (laterally interpolated) 2-D cross-correlation grid. The key result behind this algorithm is that the magnitude gradient vectors' final approach to the true peak is orthogonal to the zero-phase contour. This leads to a 2-D robust projection on the zero-phase contour that results in subsample accuracy at interpolation levels well below those needed using previously proposed methods. A full description of the 2-D, phase-coupled approach is given, including two implementations based on a geometric projection and constrained optimization. In addition, a robust fast search algorithm that allows the localization of the true peak without the need for exhaustive search is given. Experimental validation on three data sets from speckle-generating phantoms undergoing uniform diagonal motion, uniform axial deformation, and nonuniform lateral flow is given. It is shown that estimated 2-D displacement fields obtained using the phase-coupled technique display a full range of values covering the dynamic range without evidence of quantization. In comparison, a previously published method using 1-D phase-projection after lateral interpolation produces severely quantized lateral displacement fields (at the same levels of interpolation as the 2-D, phase-coupled method).

Algorithms↗

Enhancement of tumor thermal therapy using gold nanoparticle-assisted tumor necrosis factor-alpha delivery.

Tumor necrosis factor-alpha (TNF-alpha) is a potent cytokine with anticancer efficacy that can significantly enhance hyperthermic injury. However, TNF-alpha is systemically toxic, thereby creating a need for its selective tumor delivery. We used a newly developed nanoparticle delivery system consisting of 33-nm polyethylene glycol-coated colloidal gold nanoparticles (PT-cAu-TNF-alpha) with incorporated TNF-alpha payload (several hundred TNF-alpha molecules per nanoparticle) to maximize tumor damage and minimize systemic exposure to TNF-alpha. SCK mammary carcinomas grown in A/J mice were treated with 125 or 250 microg/kg PT-cAu-TNF-alpha alone or followed by local heating at 42.5 degrees C using a water bath for 60 minutes, 4 hours after nanoparticle injection. Increases in tumor growth delay were observed for both PT-cAu-TNF-alpha alone and heat alone, although the most dramatic effect was found in the combination treatment. Tumor blood flow was significantly suppressed 4 hours after an i.v. injection of free TNF-alpha or PT-cAu-TNF-alpha. Tumor perfusion, imaged by contrast enhanced ultrasonography, on days 1 and 5 after treatment revealed perfusion defects after the injection of PT-cAu-TNF-alpha alone and, in many regions, complete flow inhibition in tumors treated with combination treatment. The combination treatment of SCK tumors in vivo reduced the in vivo/in vitro tumor cell survival to 0.05% immediately following heating and to 0.005% at 18 hours after heating, suggesting vascular damage-mediated tumor cell killing. Thermally induced tumor growth delay was enhanced by pretreatment with TNF-alpha-coated gold nanoparticles when given i.v. at the proper dosage and timing.

Animals↗

Dual-mode ultrasound phased arrays for image-guided surgery.

A 64-element, 1 MHz prototype dual-mode array (DMUA) with therapeutic and imaging capabilities is described. Simulation and experimental results for the characterization of the therapeutic operating field (ThxOF) and imaging field-of-view (IxFOV) for a DMUA are given. In addition, some of the special considerations for imaging with DMUAs are given and illustrated experimentally using wire-target arrays and commercial, quality-assurance phantoms. These results demonstrate what is potentially the most powerful advantage of the use of DMUAs in image-guided surgery; namely, inherent registration between the imaging and therapeutic coordinate systems. We also present imaging results before and after discrete and volumetric HIFU-induced lesions in freshly-excised tissues. DMUA images consistently show changes in echogenicity after lesion formation with shape and extent reflecting the actual shape of the lesion. While changes in echogenicity cannot be used as an indicator of irreversible HIFU-induced tissue damage, they provide important feedback on the location and extent of the expected lesion. Thus, together with the self-registration property of DMUAs, lesion images can be expected to provide immediate and spatially-accurate feedback on the tissue response to the therapeutic HIFU beams. Based on the results provided here, the imaging capabilities of DMUAs can add unique features to other forms of image guidance, e.g. MRI, CT and diagnostic ultrasound.

Animals↗

Noninvasive estimation of tissue temperature via high-resolution spectral analysis techniques.

We address the noninvasive temperature estimation from pulse-echo radio frequency signals from standard diagnostic ultrasound imaging equipment. In particular, we investigate the use of a high-resolution spectral estimation method for tracking frequency shifts at two or more harmonic frequencies associated with temperature change. The new approach, employing generalized second-order statistics, is shown to produce superior frequency shift estimates when compared to conventional high-resolution spectral estimation methods Seip and Ebbini (1995). Furthermore, temperature estimates from the new algorithm are compared with results from the more commonly used echo shift method described in Simon et al. (1998).

Algorithms↗

Post-beamforming second-order Volterra filter for pulse-echo ultrasonic imaging.

We present a new algorithm for deriving a second-order Volterra filter (SVF) capable of separating linear and quadratic components from echo signals. Images based on the quadratic components are shown to provide contrast enhancement between tissue and ultrasound contrast agents (UCAs) without loss in spatial resolution. It is also shown that the quadratic images preserve the low scattering regions due to their high dynamic range when compared with standard B-mode or harmonic images. A robust algorithm for deriving the filter has been developed and tested on real-time imaging data from contrast and tissue-mimicking media. Illustrative examples from image targets containing contrast agent and tissue-mimicking media are presented and discussed. Quantitative assessment of the contrast enhancement is performed on both the RF data and the envelope-detected log-compressed image data. It is shown that the quadratic images offer levels of enhancement comparable or exceeding those from harmonic filters while maintaining the visibility of low scattering regions of the image.

Algorithms↗