Biomedical subjects

E Zimmermann-Nielsen

Publications and source records attributed to E Zimmermann-Nielsen.

4 recordsLinked to original sources

PubMed2001

ELISA for evaluating the incorporation of plasma derived complement split-products C3b/iC3b into solid-phase immune complexes.

An ELISA that measures plasma derived complement (C) split-products C3b/iC3b deposited on solid-phase immune complexes during C activation is described. Plates are coated with BSA, anti-BSA and plasma is added. Deposited C3b/iC3b is then detected by biotinylated anti-C3c-antibodies, avidin-alkaline phosphatase and para-nitrophenylphosphate. A novel feature is that the assay measures residual C activation capacity rather than in vivo generated C activation products. The assay was applied to plasma from 250 healthy blood donors. No difference in activation capacity of either the alternative (AP) or classical pathway (CP) with regard to age or gender was demonstrated. The total coefficient of variation was <5.7%. The ELISA procedure was compared to a standard hemolytic complement CH(50) assay using plasma from 23 out-patients with systemic lupus erythematosus (SLE). There was a weak correlation between the two assays for both C pathways, but neither the ELISA nor the CH(50) assay showed any correlation with the diagnostic ACR-criteria for SLE. However, the capacity of the CP was significantly reduced in SLE out-patients compared to healthy blood donors (P<0.0001).

Antigen-Antibody Complex↗

PubMed1999

Perioperative functional activity of the alternative pathway of complement in patients with colonic cancer.

OBJECTIVE: To investigate the functional capacity of the alternative pathway of complement in patients with cancer of the colon before, during, and after operation. DESIGN: Prospective study. SETTING: One university and two district hospitals, Denmark. SUBJECTS: 28 patients having elective or emergency operations for colonic cancer. INTERVENTIONS: Measurements of C3b fixing capacity of the alternative complement pathway in serum before, during, and after operation. MAIN OUTCOME MEASUREMENTS: The functional capacity of the alternative pathway of complement, and changes during operation. RESULTS: The functional capacity of the alternative pathway in patients with cancer of the colon was above normal (p < 0.0001 for both men and women), and the capacity remained unchanged during operation despite dilution of serum peroperatively. CONCLUSION: The alternative pathway seems to be the only immunological variable that has so far been shown to have increased functional capacity in patients with cancer, and that remains unaltered (mean value) during operation. The importance of retaining normal function of the alternative complement pathway in the prevention of postoperative infective complications and recurrence of cancer has not yet been elucidated.

Adult↗

PubMed1997

[Effects of tobacco smoking on the incidence of flap necrosis after mastectomy].

One hundred and fifty-nine women undergoing mastectomy (simple or modified radical) were followed for the development of flap necrosis. The incidence of flap necrosis was found to 14%. The only significant risk factor for the development of flap necrosis was found to be smoking, which increased the risk by 4.9 times. The effects of nicotine and carbon monoxide, the two principal known detrimental factors in tobacco smoke, are described. Prophylactic efforts are advised.

Cicatrix↗

PubMed1996

[HELLP syndrome].

A case of HELLP syndrome (Haemolysis, Elevated Liverenzymes, Low Platelets) (HS) a severe form of preeclampsia is presented. The case demonstrates that the syndrome is unpredictable, refers to differential diagnoses and pregnancy-induced abnormalities in the blood picture, and this points out the need for knowledge and awareness of the symptoms, clinical and laboratory signs of beginning HELLP syndrome.

Adult↗