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Biomedical subjects

E Yoshinaga

Publications and source records attributed to E Yoshinaga.

At least 19 recordsLinked to original sources
PubMed2000

Rapid, sensitive and simple detection of candida deep mycosis by amplification of 18S ribosomal RNA gene; comparison with assay of serum beta-D-glucan level in clinical samples.

We evaluated the clinical usefulness of a polymerase chain reaction (PCR) assay amplifying the 18S ribosomal RNA gene of fungi for the diagnosis of deep candidiasis, compared with that of the beta-glucan test or Cand-Tec test. Thirty critically ill patients who had received prolonged care with intravenous hyperalimentation and endotracheal intubation in the intensive care unit and were suspected of having deep fungal infections were examined. Twenty-one were fungi positive in the PCR assay (70%). Among 24 samples in which the PCR assay, beta-glucan test and Cand-Tec test were performed simultaneously, 75% of the samples (18/24) were fungi positive in the PCR assay, whereas only 54% (13/24) had positive reactions in the beta-glucan test and 21% (5/24) in the Cand-Tec test. The results of the Cand-Tec test showed no relationship with those of the PCR or beta-glucan test. The lower limit of detection in the PCR assay was 4-5 CFU/ml of C. albicans in blood. No fungal organism was amplified from the serum of 20 healthy individuals. The results of the PCR assay and beta-glucan test showed a significant correlation in this study, but the PCR assay proved to be more sensitive than the beta-glucan test (p < 0.05), and to be more useful for the clinical diagnosis and monitoring of deep Candidiasis.

Candida↗
PubMed1989

[Examination of periodontal disease with gingival crevicular fluid. Correlation between capacitance and clinical findings].

An intimate relationship between the inflammatory state of the gingiva and the amount of gingival crevicular fluid (GCF) is well known. For the measurement of the volume of GCF, filter paper has been used in the past. In the present study, an electrostatic capacity measuring apparatus with sensor that is insertable into gingival sulcus was fabricated. Capacitance of GCF was measured in patients with periodontitis and the following results were obtained. 1. The measuring apparatus was controlled by a personal computer to obtain the data every 0.1 second. As the GI increased at the site of measurement, higher values were obtained, with longer persistence of the trend of increase in the measured value. 2. In view of the stability of the measured values, the amount of GCF appeared to be best expressed as the value obtained after 10 seconds. 3. The correlation between the capacitance and the clinical findings was evaluated at 500 sites. The correlation was the highest in GI followed by P1I, GBI and PD, suggesting the utility of this method in the detection of initial gingival inflammation.

Electrolytes↗
PubMed1988

[Analysis of gingival crevicular fluid. Relation of isoelectric focusing protein patterns to clinical evaluation].

The purpose of this study was to determine the protein patterns in gingival crevicular fluid relation to the isoelectric focusing protein patterns of GCF and to clinical evaluations. GCF was collected with filter paper from 105 subjects. The probing depth, the gingival index (Löe & Silness) and the plaque index (Silness & Löe) as clinical evaluations The results follow: 1. The main isoelectric focusing protein patterns of GCF were between pH 5.5 and 7.5. In comparison, the GCF and the serum from the same patients showed patterns to similar serum albumin. 2. Between of GCF pH 5.5 and 7.5 the protein patterns that ranged over 60% was pI 5.65, 6.45, 6.55, 6.75 and 7.00. The frequencies of the ranges of protein patterns and clinical evaluation were compared by the X2 test. pI 5.65, 6.45, 6.55 and 6.75 and PD were significant different, as were pI 6.45, 6.55 and 6.75 and GI. But each pI and PIl. were not significantly different.

Gingival Crevicular Fluid↗