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Biomedical subjects

E Wong

Publications and source records attributed to E Wong.

276 records · Page 16Linked to original sources
PubMedDate not supplied

Retrospective study of ketotifen protective action in different allergopathies.

There is no doubt that ketotifen has continued to be an effective prophylactic anti-allergic drug throughout the years since its discovery. Therefore, we decided to perform a retrospective study of this pharmacologic agent to evaluate its efficacy, duration of treatment, as well as its tolerance in different allergic processes. A total of 1,000 histories of allergic patients were reviewed and 450 were selected by chance from 1981 through 1984. The patients were divided as to their diagnostic etiology, whether affected by pollen or house dust. Ketotifen efficacy was graded from 0-3 (bad, regular, good and excellent), according to its clinical evaluation. In general, children responded better to ketotifen treatment than adults (reduction in other symptomatic drug intake), although statistical significance was found in both populations. However, drug efficacy was better in pollen than house dust. Induced asthma and rhinitis in the children population. On the other hand, no statistical difference was found between sensitization to house dust or pollens in the adult population. To be truly effective, ketotifen should be taken for at least 3 months or more. We only found 12 (2.66%) patients who presented somnolence: the rest tolerated the drug well, with no secondary side effects.

Adult↗
PubMedDate not supplied

Indomethacin induced vasculitis.

We present a case of a 22 year old male with a personal history of ankylopoyetic spondylitis who has presented in the last year cutaneous lesions characterized by papulo erythematous (palpable purpura) localized in both distal inferior extremities in 3 occasions. These 3 episodes have always coincided with the administration of indomethacin due to increasing articular discomfort. The anatomical pathology of the lesions demonstrated the typical finding of leukocytoclastic vasculitis. The pathogenesis of the cutaneous process is discussed, considering the intervening immunocomplex wherein indomethacin or its metabolites have formed part in its origin.

Adult↗
PubMedDate not supplied

Predictive value of airway resistance in patients affected with pollen rhinitis.

We selected 38 symptomatic pollen-sensitive rhinitic patients without bronchial pathology and with normal pulmonary functional study (volume flow curve). A control group consisting of 10 individuals was studied adjacently. The study was performed within the pollen season. The determination of Raw value was carried out through increasing doses of histamine challenge employing the Apta-Jaeger apparatus. The results were expressed in PC20 wherein 26% of the pollen-affected patients presented bronchial hyperreactivity (positive histamine bronchial provocation test). In this group the values of basal Raw were found to be increased with respect to the control group (p less than 0.025) and pollen-sensitive patients with negative histamine bronchial provocation test (p less than 0.01). On the other hand, the pollen-sensitive group with negative response to histamine bronchial challenge presented Raw values which were similar to the control group.

Adult↗
PubMedDate not supplied

Distribution of IgG subclass antibodies anti-ENA (extractable nuclear antigens) in systemic rheumatic diseases.

The subclass distribution of anti-ENA antibodies was investigated in the serum of patients with systemic rheumatic diseases, using an ELISA technique. All the sera studied were previously detected positive for anti-ENA antibodies by other technique (gel precipitation). The patients were classified according to the enzymatic sensitivity of the antigen against the antibodies. There was no subclass restriction for antibodies against Ribonuclease-sensitive ENA antigen or other ENA antigens. Progressive studies showed that a single pattern of subclass distribution for each serum was maintained.

Antibodies, Antinuclear↗
PubMedDate not supplied

Generation of monoclonal antibodies to human chorionic gonadotropin by a facile cloning procedure.

A facile hybridoma procedure has been used to generate monoclonal antibodies to alpha- and beta-subunits of human chorionic gonadotropin (HCG). The procedure is based on the method of Davis et al. (1982, J. Immunol. Methods 50, 161) and involves the use of a semisolid medium containing methylcellulose for the initial cloning of hybrid cells following immunization and cell fusion. Seven to ten days after cell fusion, viable hybrid clones were removed for subculture in a liquid medium containing RPMI 1640 and 15% fetal calf serum. Initial screening of hybrid cell lines that secrete antibodies to HCG was performed on microplate enzyme-linked immunoassay (ELISA) using HCG-coated microtiter plates. The specificity of these antibodies to either alpha- or beta-subunits was determined by the sodium dodecyl sulfate-gel/protein blot radioimmunobinding method which separates alpha- and beta-subunits of HCG on nitrocellulose strips for radioimmunobinding assay. As a result of this study, it has been possible to generate about 272 hybrid cell lines that secrete antibodies reacting with either the alpha- or beta-subunit of HCG in about 5 weeks. The association constants and cross-reactivities to luteinizing hormone for some of the HCG monoclonal antibodies were determined. The high affinity and specificity of these monoclonal antibodies permit their clinical application in a sensitive sandwich solid-phase enzyme-linked and radioimmunoassay of HCG.

Animals↗