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Biomedical subjects

E Wong

Publications and source records attributed to E Wong.

At least 253 records · Page 14Linked to original sources
PubMed1977

Analysis of 4-methyloctanoic acid and other medium chain-length fatty acid constituents of ovine tissue lipids.

Medium chain-length (C6-C9) fatty acids in depot, intramuscular, kidney, and liver lipids from pasture-fed sheep and subcutaneous and intramuscular lipids from barley-fed sheep were analyzed with emphasis on the 4-methyloctanoic acid (hircinoic acid) content. Within individual animals, the level of hircinoic acid in the subcutaneous fat was usually higher than that either in the deeper depot fats or in the intramuscular fat. Variation of hircinoic acid levels between animals was greatest in the subcutanous fat and least in the perinrphric fat. There were usually higher levels of both branched and odd carbon chain fatty acids in the lipids of barley-fed sheep than in the corresponding lipids of pasture-fed sheep.

Adipose Tissue↗
PubMed1975

Purification and kinetic properties of chalcone-flavanone isomerase from soya bean.

The chalcone-flavanone isomerase from soya bean seed has been purified 8300-fold. A molecular weight of 15,600 plus or minus 1000 has been determined for the enzyme. Effects of iodoacetamide and sodium tetrathionate on the enzyme, and pH dependence of the catalytic step, indicate that a sulphydryl group is not involved in the reaction mechanism and the catalytic group is probably an imidazole side chain in the basic form. The kinetics of the isomerisation of isoliquiritigenin to liquiritigenin have been examined and show that at pH 7.6 the reaction is reversible with an equilibrium constant of 37 in favour of flavanone. A number of flavonoid compounds competitively inhibit the reaction, suggesting a possible regulatory role for this enzyme in flavonoid biosynthesis.

Chromatography, DEAE-Cellulose↗
PubMed1975

Treatment of acute osteomyelitis in children by closed-tube irrigation: a reassessment.

The addition of closed-tube irrigation to incision and drainage in the treatment of children with acute osteomyelitis has been found to be associated with: (a) a rate of technical complications of 60%, the most common complications being leaking and plugging of the tube; (b) a 30% rate of superinfection at the site of the wound, the most common organism being Pseudomonas; and (c) an average postoperative hospital stay of 30 days compared with only 16 days for the patients treated by incision and drainage alone. Suction-irrigation in the treatment of acute osteomyelitis in children does not appear to be essential for successful management, and introduces added risks of superinfection and technical complications.

Acute Disease↗
PubMed1974

Immunochemical studies of brain glutamate decarboxylase and GABA-transaminase of six inbred strains of mice.

Six different inbred strains of mice (C57BL/6J, CBA/CaJ, CE/J, DBA/2J, LP/J and RF/J) were compared in terms of specific activities and immunochemical properties of brain L-glutamate decarboxylase (GAD) and gamma-aminobutyrate transaminase (GABA-T), the enzymes responsible for the synthesis and degradation of GABA, respectively. GAD from the brains of the different strains was indistinguishable on the basis of specific activities, double diffusion tests, immunoelectrophoresis and inhibition by antibody. However, microcomplement fixation tests showed GAD from DBA and C57BL mice to be most distinctly different from GAD extracted from the Swiss mouse, from which the original antigen was prepared and that the enzyme from the CE, LP and RF also differed. Similar fixation curves were obtained for the GAD from CBA and Swiss mice. GABA-T from the different strains was indistinguishable on the basis of all the tests employed.

4-Aminobutyrate Transaminase↗