Flow cytometry (FCM) technique was applied to rapid determination of cell number of Lactococcus garvieae. An antiserum against L. garvieae was prepared and its immunological property was examined. The present antibody would recognize some epitopes of L. garvieae with a high specificity. The optimum conditions for the FCM assay were as follows: discriminate value, 60; dilution ratio of the antiserum, 1.0 x 10(4). Calibration curve for L. garvieae cells was linear, in the range of 2.4 x 10(4)-1.5 x 10(7) cells/mL. The detection of L. garvieae in cell suspensions contaminated with Escherichia coli was carried out. A good correlation was observed in the range of 20-90% for the mixing ratio of L. garvieae. One FCM assay could be completed within 2 min, and the total assay time, including the preparation of bacterial sample, was within 3 h.

Animals↗

PubMedDate not supplied

Effect of a subdural air collection on the sensory evoked potential during surgery in the sitting position.

Fifty-three cases with posterior fossa surgery were monitored using sensory evoked potentials (SEPs) and auditory evoked potentials (AEPs) simultaneously. Nine of them operated in the sitting position showed typical SEP changes during intracranial manipulations that were not attributable either to surgical or to anaesthetic handling. The SEP amplitude rapidly recovered when the patients were laid supine after operation. Skull X rays revealed intracranial air collections beneath the recording electrodes. The SEP changes were considered to be caused by conduction hindrance by the subdural air. In 1 patient, a way to overcome the air problem was demonstrated: bitemporal recording (T3-T4 montage) revealed little change in the SEP despite subdural air while the simultaneously recorded parieto-frontal record showed a drastic change.

Adolescent↗

PubMedDate not supplied

Determination of phosphate ions with an enzyme sensor system.

An enzyme sensor system for the determination of phosphate ions was constructed using immobilized enzymes and an oxygen electrode. The principle of this method is based upon the nucleoside phosphorylase catalyzed reaction for which the presence of inorganic phosphorus is indispensable. One assay could be completed within 3 min. This enzyme sensor was able to withstand at least 70 assays. This system was applicable to simple, rapid and continuous determination of phosphate ions in food.

Biosensing Techniques↗

PubMedDate not supplied

The effect of temperature on the electrochemical properties of copper-DNA membranes.

The influence of temperature, electrode plate metals and protamine on the membrane potential of an electrochemically prepared copper-DNA (Cu-DNA) membrane (size, 2.5 X 6 cm; thickness, 80 micron; Cu/P molar ratio, 0.4) was investigated. The results obtained showed that the membrane potential increased with temperature as well as with increasing order of ionization tendency of the divalent metals used, and decreased with an increase of protamine bound to the membrane. These results indicated that electrons accumulated on the anode side, and positive holes formed on the cathode side, of a Cu-DNA membrane prepared by electrolysis.

Copper↗

PubMedDate not supplied

[Intraoperative monitoring of the spinal cord. Personal results and an inventory of the current status].

The concept of intraoperative monitoring as continuous surveillance of sensory pathways by intraoperative sensory evoked potentials is described. The need for intraoperative monitoring is derived from the morbidity of certain spinal operations. First experiences from 25 own cases are outlined and the advantages of different methods discussed. The current status of spinal cord monitoring is briefly reviewed.

Afferent Pathways↗

PubMedDate not supplied

Cardiomyocyte transplantation in a porcine myocardial infarction model.

Transplantation of cardiomyocytes into the heart is a potential treatment for replacing damaged cardiac muscle. To investigate the feasibility and efficiency of this technique, either a cardiac-derived cell line (HL-1 cells), or normal fetal or neonatal pig cardiomyocytes were grafted into a porcine model of myocardial infarction. The myocardial infarction was created by the placement of an embolization coil in the distal portion of the left anterior descending artery in Yorkshire pigs (n = 9). Four to 5 wk after creation of an infarct, the three preparations of cardiomyocytes were grafted, at 1 x 10(6) cells/20 microL into normal and into the middle of the infarcted myocardium. The hearts were harvested and processed for histologic examinations 4 to 5 wk after the cell grafts. Histologic evaluation of the graft sites demonstrated that HL-1 cells and fetal pig cardiomyocytes formed stable grafts within the normal myocardium without any detrimental effect including arrhythmia. In addition, a marked increase in angiogenesis was observed both within the grafts and adjacent host myocardium. Electron microscopy studies demonstrated that fetal pig cardiomyocytes and the host myocardial cells were coupled with adherens-type junctions and gap junctions. Histologic examination of graft sites from infarct tissue failed to show the presence of grafted HL-1 cells, fetal, or neonatal pig cardiomyocytes. Cardiomyocyte transplantation may provide the potential means for cell-mediated gene therapy for introduction of therapeutic molecules into the heart.

Animals↗

PubMedDate not supplied

[Selection and evaluation of the teacher of nursing].

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Brazil↗

PubMedDate not supplied

[Ethical and legal aspects of research in nursing].

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Brazil↗