Search PubMed⌕ Search

Biomedical subjects

E Varela

Publications and source records attributed to E Varela.

35 records · Page 2Linked to original sources
PubMed1997

Determination of D-lactate by enzymatic methods in biological fluids: study of interferences.

Analysis of nondeproteinized samples with an enzymatic method to determine D-lactate indicated interferences. The presence of L-lactate dehydrogenase (LD) and L-lactate in the sample led to underestimation of D-lactate content when a sample blank was processed and overestimation when it was omitted. We proved that this interference is not due to lack of D-LD stereospecificity. Moreover, assessment of D-LD and L-LD KM for NAD+ allowed us to rule out the different affinities for this coenzyme as a cause of the interference. Our results underline the importance of deproteinizing samples for D-lactate analysis when enzymatic methods are used. The ultrafiltration procedure we propose is convenient and shows acceptable mean recovery (108%) and good imprecision (within-run CV = 4.2% and 3.0% for D-lactate at 31 and 107 mumol/L, respectively; between-run CVs were 7.3% at 49 mumol/L D-lactate and 3.1% at 115 mumol/L D-lactate).

Body Fluids↗
PubMed1994

Lipoid proteinosis of the small bowel.

We describe a 65-year-old-man who presented with acute gastrointestinal bleeding secondary to massive submucosal deposits of hyaline material in the small bowel. The histochemical and ultrastructural features of the hyaline substance were typical of lipoid proteinosis, a rare cutaneous disorder in which, to our knowledge, symptomatic compromise of internal organs has not been described previously. The patient was later found to have mild but characteristic mucocutaneous lesions of lipoid proteinosis, as well as asymptomatic deposits in other gastrointestinal sites. Our case documents that severe visceral involvement may occur in lipoid proteinosis, even in previously undiagnosed patients with mild cutaneous manifestations of the disease.

Hemorrhage↗
PubMed1994

[Do the physical characteristics of radiopaque markers modify the results of the measurement of colonic transit time?].

UNLABELLED: This study was performed in 15 healthy control subjects (8 males- age 23-70 and 7 females age 20-74) as a way to compare the total and segmental colonic transit time with two different type of radiopaque markers. One type of markers was 3 mm cut sections of a radiopaque polietilene 14 French Levine tube, with a medium weight of 64.3 mg and a specific gravity (SG) of 1.28. The other type were 7 mm lenticular insoluble barium (lentils) with medium weight of 231 mg and a SG of 1.87. Each subject ingested 20 markers of each type with the breakfast and front and profile plain films of the abdomen were taken 24 and 48 hs after. The total colonic transit time for the polietilene markers was 17.7 hs and 27.3 hs for the barium type (BM) (p < 0.001). The segmental transit time for right colon was 2.4 hs (PM) and 9.7 hs (PM) and 9.7 hs (BM) (p < 0.001) in the left colon 9.5 hs (PM) and 11.3 hs (BM) and in rectoanun 5.9 hs (BM) with no statistical significance. CONCLUSION: the physical characteristics of the radiopaque markers can modify the results of the colonic transit times.

Adult↗
PubMed1993

[The ICD-10 and mood disorders].

30 patients with affective disorders are evaluated by a Mental Health team following diagnostic criteria of ICD-10 and DSM-III-R. Results are compared. Significative aspects of ICD-10 and differences between both classifications are analyzed in this area.

Adult↗
PubMed1992

[Manometric effects of pinaverium bromide in irritable bowel syndrome].

The effects of pinaverium bromide on colonic motility were investigated in a controlled, controlled, cross-over study in 32 patients with irritable bowel syndrome. Constipation was clearly predominant in one group of 16 patients, and diarrhea in the other group of 16. Manometric measurements were taken of the colonic motor response generated by distention of a balloon inserted to the rectosigmoid junction. Measurements were taken before and one hour after ingestion of two tablets containing placebo or two tablets each containing 50 mg of pinaverium bromide. Following intake of placebo the motility index increased from the basal value in patients with constipation, and resistance to distention decreased in the diarrhea group. These changes were attributable to repetition of the mechanical stimulus within a relatively brief time lapse, or more probably to the ingestion of liquid which accompanied intake of tablets. Compared with placebo, pinaverium bromide induced inhibition of both effects. From the therapeutic point of view, the decrease in motility index seen in patients with irritable bowel syndrome and constipation is particularly interesting.

Adult↗
PubMed1991

[Results of perineal electric stimulation in stress urinary incontinence].

The results achieved with perineal electrical stimulation in 25 patients with stress urinary incontinence of different etiology are presented herein. Overall, positive results were achieved in 52% and 28% were nonresponders. The results were positive in 66% with post-prostatectomy urinary incontinence. Before recurring to other more complicated therapeutic procedures, this treatment modality could be an alternative in the treatment of stress urinary incontinence.

Adult↗
PubMed1990

[Treatment of bladder instability using intravaginal electric stimulation (intrarectal)].

Fourteen patients (13 females, 1 male) with urinary incontinence from bladder instability were treated with intravaginal (intrarectal) electrical stimulation. Good results were achieved in 57.1% of the cases. The foregoing finding shows that intravaginal (intrarectal) electrical stimulation may constitute a therapeutic option in urinary incontinence from bladder instability.

Adolescent↗
PubMed1985

Dermatomyositis-like syndrome caused by trichinae. Report of two cases.

We report 2 cases of trichinosis in which the clinical features resembled dermatomyositis. In both cases, however, the diagnosis was established histologically by demonstrating trichina larvae in the patients' muscle biopsies. Indirect immunofluorescence and counterimmunoelectrophoresis techniques were used to confirm the diagnosis serologically.

Adult↗
PubMed1980

[Fiberendoscopic sclerotherapy of esophageal varices (description of a technic)].

We describe a technique used for fiberendoscopic sclerosis of esophageal varices. The addition of a balloon around the endoscope is advocated for three uses: 1) ingurgitation of varices allows better visualization, 2) the methods avoid the quick clearing of the sclerosing agent, 3) it can be used as hemostatic agent in case of necessity. The sclerosant agent used is an association of ethanolamine oleate, hypertonic glucose solution (50%) and natural conjugated strogens.

Esophageal and Gastric Varices↗
PubMedDate not supplied

[First isolation of Histoplasma capsulatum from the urban bat Eumops bonariensis].

We report the first isolation of Histoplasma capsulatum var. capsulatum from a male bat Eumops bonariensis captured in Buenos Aires city in 2003. The pathogen was recovered from spleen and liver specimens, and was identified by its phenotypic characteristics. PCR with primers 1283, (GTG)5, (GACA)4 and M13 was used to compare both bat isolates with 17 human isolates, 12 from patients residing in Buenos Aires city, and 5 from other countries of the Americas. The profiles obtained with the four primers showed that both bat isolates were identical to each other and closer to Buenos Aires patients than to the other isolates (similarity percentage: 91-100% and 55-97%, respectively). The high genetic relationship between bat isolates and those from patients living in Buenos Aires suggests a common source of infection. This is the first record of E. bonariensis infected with H. capsulatum in the world, and the first isolation of the fungus in the Argentinean Chiroptera population. In the same way as these wild mammals act as reservoir and spread the fungus in the natural environment, infection in urban bats could well be associated with the increase in histoplasmosis clinical cases among immunosuppressed hosts in Buenos Aires city.

Americas↗