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Biomedical subjects

E Theilade

Publications and source records attributed to E Theilade.

At least 37 records · Page 2Linked to original sources
PubMed1983

Regional variations in viable bacterial and yeast counts of 1-week-old denture plaque in denture-induced stomatitis.

Quantitative cultural studies of yeasts and bacteria were made from 1-week-old denture plaque accumulated on pieces of self-adhesive tape stuck on the fitting surface of the maxillary denture in four well-defined locations. A tape piece was also stuck on the buccal denture flange. The cultural examinations were made in 15 patients with denture-induced stomatitis which affected most of the denture-bearing mucosa. There was no significant difference of the bacterial counts from the different sampling areas although the variations between patients was significant. The yeast counts from the test area located on the buccal denture flange were significantly lower than those originating from the fitting denture surface. On the other hand, there was no significant variation of the yeast counts when comparing the test areas of the fitting denture surface. In 12/15 of the patients yeast counts of greater than or equal to 10(3)/cm2 were obtained from the fitting denture surface. The results indicate that the environmental conditions beneath a denture base predispose for yeast colonization and are different from those present on the buccal flange.

Bacteria↗
PubMed1982

Predominant cultivable microflora of human dental fissure plaque.

Plaque developed in 10 occlusal fissures from unerupted third molars during implantation for 200 to 270 days in lower molars of dental students was studied. To characterize the predominant cultivable flora, 592 isolates (51 to 67 from each fissure) were subcultured from anaerobic roll tubes. Twenty-eight of the isolates were lost. Streptococci constituted 8 to 86% (median, 45%) of the isolates, Streptococcus mutans constituted 0 to 86% (median, 25%) and S. sanguis constituted 0 to 15% (median, 1%). A few isolates of "S. mitior" and "S. milleri" were found, but no S. salivarius. Staphylococci made up 0 to 23% (median, 9%). Gram-positive rods constituted 6 to 59% (median, 35%). Of these, 0 to 46% (median, 18%) were Actinomyces naeslundii and A. viscosus, but no anaerobic actinomyces were isolated. Arachnia and propionibacteria made up small proportions, lactobacilli were isolated from two fissures, constituting 10 and 29%, and eubacteria were isolated from one fissure (27%). Gram-negative cocci made up 0 to 46% (media, 4%). Only two isolates of gram-negative rods were found, both facultative anaerobes. Although 8 of the 10 fissures had large proportions of S. mutans, lactobacilli, or both, no caries was found even with microradiography. The large individual variation probably reflects differences in initial colonization from saliva and in growth conditions in each fissure.

Bacteria↗
PubMed1981

Method for studying the development, structure and microflora of denture plaque.

Pieces of sandblasted, self-adhesive tape were stuck to the fitting surface of maxillary dentures in 17 edentulous subjects with clinically normal palatal mucosa. After 1 week, during which the subjects abstained from denture hygiene, the tape samples were removed for cultural and microscopical examinations. The microorganisms were suspended in RTF by ultrasonic treatment and total viable anaerobic counts and viable counts of yeasts per cm2 of tape were calculated after inoculation of serial dilutions on nonselective medium and sabouraud agar. Total anaerobic viable counts ranged from 4 X 10(4) to 5 X 10(8). By repeated sampling the largest intra-individual variation was 10-fold, only. Yeast counts constituted less than 1% of the total viable counts in all but two of the subjects. Light and electron microscopy showed a bacterial plaque of predominantly cocci and rods.

Aged↗
PubMed1976

Haemophili in developing dental plaque.

Evidence for a possible role played by oral haemophili in the development of dental plaque was sought by studying the occurrence of these bacteria in early dental plaque of smooth surfaces and occlusal fissures in six dental students. The mean number of haemophili per 10(3) anaerobes in early smooth surface plaque (18 h) and fissure plaque (7 d) was 95 and 22 respectively. Examination of 988 Haemophilus isolates revealed that H. parainfluenzae was the only species in samples of fissure plaque, whereas some samples from smooth surfaces, in addition to the predominating and ubiquitous H. parainfluenzae, yielded growth of the two species H. segnis and H. aphrophilus. It is concluded that haemophili are among the primary colonizers of smooth surfaces of teeth.

Dental Plaque↗
PubMed1975

Experimental gingivitis in young and elderly individuals.

The development of experimental gingivitis was studied in young elderly humans during a 21-d period of oral hygiene abstention. The state of the gingiva was assessed by the Gingival Index and by measurements of the amount of gingival exudate on filter paper strips placed at the entrance of the gingival sulcus of the lower lateral incisors and cuspids. Soft deposits were assessed by the Plaque Index and by differential counts of microorganisms in gram stained smears od ento-gingival plaque. At the end of the plaque growth period, the patients were given a thorough dental prophylaxis. Gingival condition and plaque were assessed at regular intervals during a subsequent period of controlled oral hygiene. The development of gingivitis during the oral hygiene abstention period was more rapid and more severe in old than in young individuals. Plaque accumulation was greater in the older persons. A definite difference in plaque consistency was alos observed. However, microscopic counts of various types of microorganisms did not reveal any differences throughout the period of plaque accumulation. When active oral hygiene was reinstituted, the state of the gingiva rapidly returned to pre-experimental levels in both groups. The findings of this study indicate that with age ther is an altered host response to the microorganisms of the plaque.

Adult↗