Biomedical subjects
Publications and source records attributed to E Tal.
Male dogs were injected i.v. with 1 methyl-4-phenyl-1,2,3,6-tetrahydro- pyridine (MPTP) 2.5 mg/kg twice with an interval of 6 days. Twenty minutes after MPTP injection cyclohexyladenosine (CHA) 1 mg/kg i.v. was injected. Ten minutes after CHA injection the MTPT induced tremor, tachycardia and salviation ceased.
In 70 patients who were between 32 and 36 weeks' gestation and who complained of painful uterine contractions, real-time ultrasonography was performed to establish the presence or absence of fetal breathing. Ten of the 14 women in whom no fetal breathing movement could be detected were delivered of infants within 48 hours, whereas the pregnancies continued for a week or longer in 52 of the 56 patients in whom fetal breathing was present. There was a good correlation between Bishop score and pregnancy outcome. Of the 48 patients with "unfavorable" cervix (Bishop score less than or equal to 3), 45 were delivered of infants after 1 week or more, whereas of 22 patients with a Bishop score of 4 to 6, nine were delivered of infants within 48 hours and ten pregnancies continued for 1 week or more.
1. The response of TSH to TRH and the TRH content of the hypothalamus, following indomethacin, ibuprofen and paracetamol treatment, was measured in male rats. 2. Daily treatment of indomethacin (3 mg/kg)) for 3 days markedly reduced T4 concentration in the serum, the TRH content of the hypothalmus gland and inhibit Pituitary TSH response to the low T4 level in the blood. 3. Ibuprofen (12 mg/kg) and paracetamol (50 mg/kg) did not influence T4 or TSH levels of the serum nor the TRH content of the hypothalmus. 4. TRH-induced TSH secretion was not influenced by indomethacin, ibuprofen or paracetamol treatment.
Parameters of peripheral blood T lymphocytes were determined repeatedly (twice, 2-4 weeks apart), in ten old (78 + 5) and compared to nine young (31 + 5) human subjects. Assays included percentage of total, helper, and suppressor, T lymphocytes, and the reaction to PHA stimulation for 24, 48, 72, and 96 h, as assessed by levels of proliferation and IL-2 production. A lower response to PHA was observed in the old as compared to the young, with no significant changes in T cell subsets. A marked variability was noted between the results of the first and second determinations of the response to PHA in each individual. The lack of correlation between the two determinations was more prominent in the old. Unresponsiveness to PHA throughout the incubation period, was noted in two old subjects, but, in only one of the two determinations. This transient unresponsiveness was not accompanied by any changes in their clinical state. Thus, establishments of the immune status of the aged should be based on at least two repeated determinations.
The effect of intraperitoneally (i.p.) and intragastrically (i.g.) administered ethanol solution, and the influence of voluntary ethanol uptake (20% v/v) on adrenocortical activity of adult male rats was studied. Both i.p. and i.g. ethanol administration resulted in a significant activation of adrenocortical mechanisms, while voluntary ethanol uptake failed to induce elevation of serum corticosterone concentration. No difference was found in blood ethanol concentration among these groups. The responsiveness of adrenocortical mechanisms was also tested in rats which were given the free choice between ethanol solution (5% v/v) and tap-water for three weeks. Unavoidable electric foot-shocks, as stressor, resulted in an elevation of serum corticosterone concentration in control animals, but this response was found to be significantly reduced in chronically ethanol drinking rats.
Male rat thyroid glands were incubated for two hours in Krebs-Ringer bicarbonate buffer with different amounts of morphine and/or naloxone. Five micrograms/ml morphine produced a significant increase in the T4 concentration of incubation medium, and resulted in an accumulation of cAMP in the tissue. Naloxone did not change the T4 release but its incubation with morphine prevented the morphine-induced changes. Similarly, naloxone inhibited the morphine-induced accumulation of cAMP in the thyroid tissue.
Avoidance learning and pain sensitivity were studied in rats after chronic ethanol administration (1.1 to 1.7 g per kg b.w. in drinking water) at prenatal, adolescent and adult ages. The behavioural reactions were tested in adulthood by studying passive and active avoidance learning and the threshold of pain sensitivity to electric tail shock. Chronic ethanol consumption led to an impairment of avoidance learning and to hyperalgesia in each experimental series, although the alterations were greater in the prenatally treated groups. The experimental observations are discussed in the light of fetal alcohol syndrome.
[4,5(3)H]L-Leucine incorporation into proteins in placental slices of alcoholic and normal women was measured in the first and second trimesters of pregnancy, and on the day of birth. In this study, alcoholic women were defined as women who drink daily at least 150 ml, calculated as 100% ethanol. Leucine incorporation into proteins was significantly decreased in alcoholic women compared with normal placentas: 72% in the first, 70% in the second trimester, and 82% in term placenta. The data indicate that not only can chronic maternal alcohol consumption induce defects in protein synthesis, but also that heavy alcohol ingestion, even during the beginning of pregnancy, could be toxic for the human placenta and development of the fetus.
Explore the source record for details and available documents.
Experiments were designed to demonstrate that morphine may exert a direct short-term effect on the hormone release of the thyroid gland. Groups of male rats were injected with single doses of 1, 5 and 10 mg/kg morphine, or with 2 mg/kg naloxone and in addition with morphine 30 min after naloxone and/or with naloxone 30 min after morphine pre-treatment. The rats were killed by decapitation 15, 30 and 60 min after the injection and serum was collected and stored for subsequent TSH, T4 and T3 radioassays. All doses of morphine resulted in an increase of serum T4 and T3 concentrations 15 and 30 min after the injection, with a tendency to return to control levels by the 60 min samples. Serum TSH concentrations were to suppressed by administration of 5 and 10 mg/kg but not by 1 mg/kg morphine. Naloxone treatment did not increase the T4 and T3 concentrations; however, serum TSH was elevated in the 15 min sample. Naloxone pre-treatment inhibited the morphine induced release of T4 and T3 into the serum, but naloxone administration after morphine pre-treatment failed to prevent the increase of T4 and T3 secretion. These data suggest that morphine may exert a short-term stimulatory effect on the thyroid gland with a concomitant inhibitory action on the hypothalamo-pituitary TSH system.
Explore the source record for details and available documents.
The correlation between 3',5', c-AMP levels, TSH content and secretion of separated thyrotropic cells was studied. Incubation of the separated cells with 1, 10 and 100 ng of TRH does not change the 3',5',c-AMP levels, despite the significant rises of the TSH level. Dibutyryl c-AMP causes rise in TSH content, with no indication of its secretion. PGE2 10(-5) increased 3'5',c-AMP levels with no change in the content or secretion of TSH in separated thyrotropic cells.
A sensitive and specific immunofluorescent method for identification of thyrotropic cells was developed. The TSH-producing cells were found to be heterogenic in their morphology and intensity of staining.
Explore the source record for details and available documents.
Treatment of 29 cases of chronic lymphedema of various origins, mostly of the lower limbs, by manual lymph drainage massage resulted in significant changes of neurohormone excretion in the urine, whereas the secretion of 17-KS, thyroxine, minerals, and creatinine was not significantly changed. Comparison of the values of urinalysis before and after manual lymph drainage of the patients showed the following changes: 17-KS; -3.5% (non significant); 17-OH: -31% (significant); adrenaline: +50% (significant); noradrenaline: +19% (significant); serotonin: -22% (significant); 5-HIAA: +21% (significant); histamine: +129% (highly significant); thyroxine: -17% (nonsignificant); creatinine: -17% (nonsignificant); sodium: -1% (nonsignificant); potassium: -14% (nonsignificant). The corresponding values for ten controls were all non significant. These findings underline the importance of adrenaline and noradrenaline release by manual lymph drainage, which improves circulation. On the other hand, our results indicate the involvement of histamine and perhaps of serotonin in lymphedema formation, and suggest a combination of manual lymph drainage massage with antihistamine and antiserotonin treatment.
A method for the enrichment of live thyrotrophic pituitary cells is described. Pituitary glands of young male rats were removed into Earle's solution and dispersed in a 0.1% trypsin solution containing 0.5% bovine serum albumin, pH 7.2. Nylon fibres (25 microgram) were used for the separation of the thyrotrophic cells, by stringing them across a plastic frame which fitted a plastic Petri dish containing the cell suspension. The fibres were washed with light petroleum (b.p. 60--80 degree C) and carbon tetrachloride, hydrolysed with 3 M-HCL for 30 min at room temperature and washed with distilled water and phosphate-buffered saline (pH 7.2). The fibres were treated with thyrotrophin releasing hormone (TRH) alone or in the presence of soluble carbodiimide solution. After incubation for 1 h at room temperature, the fibres were transferred to a new Earle's medium and cells were released from the fibres by plucking them with a needle. The separated thyrotrophic cells were identified by radioimmunoassay and by electron microscopy. Using the above-mentioned methods, enrichment of thyrotrophic cells was obtained. Thus, the amounts of TSH, prolactin, LH and GH released, during 2 h of incubation, by 1.5 x 10(6) unseparated cells were 6.8 +/- 0.65, 4.1 +/- 0.47, 4.8 +/- 0.52 and 5.2 +/- 0.68 microgram respectively, while the same number of purified thyrotrophic cells released 76.1 +/- 0.42, 1.2 +/- 0.3, 0.6 +/- 0.35 and 1.6 +/- 0.22 microgram of the same hormones (means +/- S.E.M.).
Explore the source record for details and available documents.
Changing weather fronts produce severe changes of daily rhythms. Electrical impulses arrive 1-2 days before the weather and create either atmospherics or "positive ions". They are responsible for the epidemic appearance of migraine and of thrombo-embolism. The impact of atmospheric electricity has been assessed in 1000 patients by daily urinalysis of serotonin, 5-HIAA, adrenaline, noradrenaline, histamine, thyroxine, 17-KS, 17-OH, Na, K, creatinine and diureses. The changes found in these 12 parameters allowed a classification of heat reactions into three clinical entities: 1. serotonin hyperproduction causing a typical irritation syndrome: 2. adrenal deficiency producing a typical exhaustion syndrome; 3. hyperthyroidism "Forme fruste" with subclinical "apathetic" thyroid symptoms. These sufferings, typical for Föhn, Tramontana, Sirocco, Sharkiye, Chamssin, Sharav and Santa Ana Winds, are mainly due to positive ionisation of the air. They can be prevented by negative ionising apparatuses or specific drug treatment.