Biomedical subjects

E Takara

Publications and source records attributed to E Takara.

21 records · Page 2Linked to original sources

PubMed1977

[A new technique for collection the cerebrospinal fluid from rat (authors transl)].

An attempt was made to collect the cerebrospinal fluid (CSF) of rat using a capillary phenomenon technique. Experiments were carried out in 76 rats anesthetized with pentobarbital sodium (Nembutal, Abbot. 50 mg/kg IP). The midline incision in the occipital region was made from 0.5 cm anterior to interauricular line of 4 cm in length. Exposing the atlanto-occipital dura mater by separating the nuchal muscles, the CSF was collected with a heparinized microtube (MICROPET. Bection, Dickson and Company) through a small hole made by a 18G syringe needle. Thus, 0.05-0.05 ml of the CSF could be readily obtained without mixture of the blood in 64 cases out of 76 trials, 84%. This simple technique is a useful way to get a small amount of CSF from little animals, such as rats.

Animals↗

PubMed1977

[A case with bilateral internal carotid and left vertebral artery occlusion (author's transl)].

Since Moniz reported the obstruction of the extracranial internal carotid artery in 1937, there have been many reports of extracranial arterial occlusion. The authors experienced a case of 40-year-old woman who had minor neurological deficit such as attack of black out, disturbance of the bilateral visual acuity, concentric narrowing of the bilateral field and disturbance of the bilateral auditory acuity, in spite of complete obstruction of both internal carotid arteries and the left vertebral artery, and possible obstruction of the right vertebral artery. The angiograms showed well developed collateral circulation. The cause of the obstruction was considered to be angitis following otitis media and meningitis in her childhood. The symptoms were alleviated by drugtherapy.

Adult↗

PubMedDate not supplied

Simultaneous in vivo measurement of lumped constant and rate constants in experimental cerebral ischemia using F-18 FDG.

Lumped and transfer rate constants in ischemic brain tissue must be measured to estimate accurately cerebral glucose utilization by the deoxyglucose method. We studied the bilateral middle cerebral artery occlusion model in 17 cats, 5 with a 1-hour occlusion, 5 with a 4-hour occlusion, and 7 with a sham operation. The time course of cerebral tissue radioactivity (Ci*(t)) was monitored by external coincidence counting during a programmed infusion of [18F]-2-deoxy-2-fluoro-d-glucose (18F-2-FDG). Arterial plasma concentration (Cp*(t)) of the tracer was kept constant during the first 45 minutes. Rate constants were estimated from Ci*(t) and Cp*(t) by a nonlinear least-squares fitting routine. The lumped constant was estimated from the fit of the ratio of extraction fractions of glucose and 18F-2-FDG by nonweighted, nonlinear least-squares fitting. In the 4-hour-occlusion model, the transfer constant k1* was 23% lower, k3* 39% lower, and the lumped constant 78% higher than in the sham-operated animals. In the 1-hour-occlusion model, k3* was 26% lower than in the sham-operated animals but the lumped constant was not significantly different. The rate of glucose utilization was significantly different in the 4-hour-occlusion model compared to the sham-operated animals (48% decrease, p less than 0.05) but was not significantly different in the 1-hour-occlusion model.

Animals↗