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Biomedical subjects

E Szydłowski

Publications and source records attributed to E Szydłowski.

7 recordsLinked to original sources
PubMed1990

Increase in cooperativity of oxygen binding as a factor related to high maximal oxygen uptake during physical effort.

In a group of trained, nonsmoking men, apparent haemoglobin oxygen affinity was assessed in vitro in the whole blood collected immediately before and after maximal physical effort. Measurements were performed using standard laboratory equipment. Maximal oxygen uptake (V O2 max) has been monitored during maximal physical effort. Increase in app Hb-oxygen affinity after exercise was found by measurements of p25 and by differences between plots of correlations between V O2 max and resting and post-exercise parameters of Hb-oxygen binding. Significant correlations were found between preexercise n50 and VO2 max (r = -0.59) and between post-exercise log P50 and V O2 max (r = 0.6). Exercise-induced increase in Hb-oxygen affinity and in cooperativity of Hb-O2 binding were found to be higher in persons with higher values of VO2 max.

Adolescent↗
PubMed1987

Physical effort-induced decrease in apparent whole blood Hb-oxygen affinity normalized for intraerythrocytic pH (pHe) at low initial values of pHe.

Effect of physical effort on haemoglobin-oxygen affinity was studied in whole blood of 28 healthy males. In persons showing decrease in pH gradient between plasma and erythrocytes after physical effort, a parallel decrease in apparent, normalized for intraerythrocytic pH (pHe), Hb-oxygen affinity was noted. An increase in pH gradient after exercise was accompanied by increase in apparent, corrected for pHe, Hb-oxygen affinity and in blood Hb content. Correlation between exercise-induced changes in Hb-oxygen affinity and in pH gradient (-0.66) was highly significant (p less than 0.001). It is suggested that both parameters may be increased by recruitment into circulation of old erythrocytes from spleen. Decrease in both parameters may be related to low values of intraerythrocytic pH before effort. Groups of persons differing in exercise-induced changes of Hb-oxygen affinity did not differ in maximal oxygen utilization.

2,3-Diphosphoglycerate↗
PubMed1983

Exercise-induced increase of methaemoglobin concentration and low cooperativity in haemoglobin-oxygen binding at rest correlate with low oxygen consumption during maximal effort.

Relations between oxygen consumption, physical effort and parameters characterizing oxygen transport by red blood cells [rbc] before and after exercise were assessed in 32 healthy persons. The oxygen affinity of haemoglobin was studied in rbc by several measurements of pO2, pCO2, pH and HbO2 in samples of heparinized venous blood equilibrated with the gas compartment containing decreasing concentrations of O2 and 5% CO2. The actual oxygen affinity significantly decreased after exercise with the values of log p50 [act] equal 1.42 +/- 0.1 at rest and 1.51 +/- 0.12 after the effort; the difference was assessed by the nonparametric test of pairs [p less than 0.05]. No effect of exercise was noted on the mean values of apparent p50, a parameter sensitive to the intraerythrocytic regulators of haemoglobin-oxygen affinity. In subjects with the highest values of efficiency of oxygen utilization [Eff], i.e. the lowest oxygen consumption during maximal effort, the values of Eff were however found to be correlated with the cooperativity in Hb-O2 binding at rest [r = 0.565] and with the exercise-induced changes in metHb concentration [r = 0.258]. The exercise-induced changes in metHb concentration correlated with the exercise-induced change in apparent p50 [r = 0.496].

Adolescent↗