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Biomedical subjects

E Suzuki

Publications and source records attributed to E Suzuki.

527 records · Page 30Linked to original sources
PubMedDate not supplied

A simple single-tube procedure of PCR assay for the detection of hepatitis C virus RNA.

We developed a simple single tube procedure using the PCR (polymerase chain reaction) for the detection of hepatitis C virus RNA. The entire reaction from RNA extraction to PCR occurred in one tube; this was made possible by the use of more than 1,000 micrograms/ml of proteinase K for RNA extraction, instead of the acid-guanidinium thiocyanate-phenol-chloroform method. All necessary reagents were added to the tube, and PCR products were not removed from the tube until the end of PCR, although opening of the tube during the procedure could not be avoided. Therefore, cross-contamination which might theoretically take place during transfer of products between tubes never occurred. This method detected about 1 chimpanzee infectious dose of HCV in H strain plasma.

Base Sequence↗
PubMedDate not supplied

Enhanced antibody production at slowed growth rates: experimental demonstration and a simple structured model.

A simple structured model for monoclonal antibody (MAb) production kinetics was formulated by combining the cell cycle theory with the estimated number of MAb-coded messenger RNA (mRNA) molecules per cell: it is assumed that the rate-controlling step is first order in this mRNA and that the growth rate variation does not alter the MAb synthesis rate within any cycle phase but only changes the relative time length of the individual phases. The model predicted "negatively growth associated" MAb production kinetics and thus an enhanced MAb production rate to be achieved by slowing the cell growth. Experiments consistent with these assumptions provided support for the model. Hybridoma cultures where growth was slowed by either a DNA synthesis inhibitor (thymidine or hydroxyurea) or by a selective inhibitor of initiation of nonantibody protein (potassium acetate) exhibited 50-130% MAb production rate enhancement for growth slowed up to 50%; however, further decreases in the growth rate also decreased the MAb production rate. Experiments inconsistent with these assumptions showed other behavior: general inhibition of protein chain elongation (by cycloheximide) or inhibition of ribosomal RNA (rRNA) synthesis (by actinomycin D) each slowed both growth and the specific MAb production rate, leading to net "positive" growth associated MAb production rates. Thus, a need for models with greater structure is also demonstrated.

Animals↗
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Glycolipids from Paracoccidioides brasiliensis. Isolation of a galactofuranose-containing glycolipid reactive with sera of patients with paracoccidioidomycosis.

In the present study, we describe the isolation of glycolipids from yeast and mycelium forms of Paracoccidioides brasiliensis. Both forms contains glucosylceramide as the only neutral glycosphingolipid and two acidic glycolipids termed band 1 and band 2. Band 1 was found to be reactive with 100% of sera of patients with paracoccidioidomycosis tested. Structural analysis of band 1 revealed that it is composed of mannose and galactose in molar ratios of 2:1, and a trace amount of glucose. Furthermore, this paper presents evidence that the galactose unit of band 1 is in the furanose configuration. Finally, it was found that reactivity of paracoccidioidomycosis sera with band 1 glycolipid can be attributed mainly to antibodies directed to galactofuranosyl residue present in this glycoconjugate.

Antibodies, Fungal↗
PubMedDate not supplied

Potential use of specific human and chicken antibodies for detection of Hanganutziu-Deicher antigen(s) in sera of cancer patients.

A human Hanganutziu-Deicher (HD) antibody and a chicken anti-N-glycolyneuroaminyllactosylceramide (HD3) antibody were compared in their reaction against HD antigen-active ganglioside (HD3) and a glycoprotein (GP) by radioimmunoassay (RIA). The human antibody had a 50 times higher reactivity with the glycoprotein, while the chicken antibody reacted equally with both antigens. Both antibodies had a higher reactivities with HD antigen(s) in sera of five of eight lung cancer patients than 54 normal human sera. Since four of the above five sera had no abnormal titers to GP, it was concluded that their immunological status was antigen excess. The chicken antibody may be useful in follow-up studies of cancer patients to correlate the expression of HD antigen in tissues and sera with the elevation of HD antibodies, offering alternative methods of clinical prognosis of tumor growth and/or metastases. The human HD antibody may also be useful for the detection of HD antigens of glycoprotein nature.

Animals↗