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Biomedical subjects

E Stolz

Publications and source records attributed to E Stolz.

At least 235 records · Page 13Linked to original sources
PubMed1983

Gonococcal serology. A comparison of three different tests.

Three serological tests for the detection of gonococcal antibodies were compared: an enzyme-linked immunosorbent assay (ELISA), an indirect haemagglutination reaction (IHA), and a gonococcal complement-fixation test (GCFT). The ELISA was performed with gonococcal pili of a Rotterdam strain (1443) as antigen, the IHA with pilus antigen of an American strain (2686, Buchanan), and the GCFT with whole gonococci of a single strain (46695, Oliver) as antigen. The tests were performed on sera from the same groups of Dutch patients; samples of sera were taken at the first examination and generally 11-22 days later. The ELISA and the IHA were more sensitive than the GCFT. The specificity of the tests was equal in low-risk groups, but the GCFT was slightly more specific in high-risk groups. The ELISA and the IHA did not differ in sensitivity and specificity. The agreement between the ELISA and IHA for patients with uncomplicated gonorrhoea was low (chi = 0.44), but the agreement between the GCFT and the two pilus assays was less (chi = 0.26 and 0.20). The sensitivities were highest for sera from patients with oropharyngeal gonorrhoea or with gonococcal complications; again the ELISA and the IHA were more sensitive than the GCFT.

Adult↗
PubMed1983

Antibodies to gonococcal pili in women with asymptomatic gonorrhoea. Diagnostic value of the ELISA for testing women attending an STD clinic.

The diagnostic efficacy of an enzyme-linked immunosorbent assay (ELISA) using gonococcal pili 6650 as antigen was studied in asymptomatic women attending an STD clinic. Of the 182 women studied, gonorrhoea was diagnosed in 29%. The value of the ELISA was calculated on the basis of four arbitrary cut-off points in the test. The best predictive values for a positive result (PV(+)) were obtained at an absorbance value of A greater than or equal to 1.15 and A greater than or equal to 1.30 and that for a negative result at A greater than or equal to 0.85. When patients with a history of gonorrhoea were excluded, the PV(+) rose only at A greater than or equal to 1.15 (not at A greater than or equal to 1.30) and the PV(-) rose slightly. To be of use in the diagnosis of gonorrhoea in asymptomatic patients the ELISA should be used as follows: the result is positive at A greater than or equal to 1.15 and negative at A less than 0.85, the PV(+) then being 0.52 and the PV(-) 0.85. Whenever sera give a result between A = 0.85 and A = 1.15, the test should be repeated.

Antibodies, Bacterial↗
PubMed1983

Enzyme-linked immunosorbent assay for detecting gonococcal antibodies using two antigenically different gonococcal pili as antigen.

Antibodies to pilar antigens of two gonococcal strains isolated in Rotterdam (6650 and 1443) were detected using enzyme-linked immunosorbent assays (ELISA). Paired sera (the first sample taken at the first examination (D1) and the second 11-22 days later (D2)) from women with and without gonorrhoea attending a sexually transmitted disease (STD) clinic were studied. The sensitivity of the ELISA using gonococcal pili 6650 as antigen (ELISA 6650) was significantly higher than that using gonococcal pili 1443 as antigen (ELISA 1443). The specificity of the two tests differed little. On D1 the sensitivity in women with uncomplicated gonorrhoea was 69% in the ELISA 6650 and 45% in the ELISA 1443; the corresponding values in asymptomatic infected women were 75% and 57% respectively. The agreement (both in positive and in negative results) between the two tests was less than might have been expected (kappa = 0.41).

Antibodies, Bacterial↗
PubMed1982

Detection of Neisseria gonorrhoeae antigen by a solid-phase enzyme immunoassay.

The Gonozyme test (Abbott Laboratories) is a new enzyme immunoassay for detecting Neisseria gonorrhoeae antigens in specimens from the urethra in men and the endocervix in women. To evaluate the usefulness of the assay 249 patients were investigated. The results obtained with the immunoassay were compared with those of culture and microscopy of Gram-stained smears. The sensitivity and specificity of the test were high in men with urethritis and acceptable in different groups of women. As the sensitivity of the Gonozyme test was much higher than that of microscopy for endocervical specimens it might be useful as a rapid screening test for the detection of gonorrhoea in women.

Antigens, Bacterial↗
PubMed1981

[Mastocytosis].

Explore the source record for details and available documents.

Adult↗
PubMed1980

Mercury exanthem as occupational dermatitis.

A patient developed an exanthem after inhalation of mercury vapors. Clinically and histopathologically the exanthem resembled an erythema exudativum multiformelike drug eruption.

Dermatitis, Occupational↗
PubMed1980

Relationship of the interval between infections and the similarity of gonococcal strains in recurrent gonorrhoea.

Paired gonococcal isolates from 38 patients with recurrent episodes of gonorrhoea at varying intervals were examined for similarity by means of gonococcal auxotyping and susceptibility to antibiotics. A different gonococcal strain was the cause of the second infection in 53% of the patients. Longer intervals between infections were significantly associated with greater numbers of dissimilar strains as a cause of the second infection. The same strain was usually found in infections occurring within 60 days of each other whereas different strains were more likely to cause infections occurring more than 60 days apart. The isolates causing the second infection were significantly more susceptible to penicillin.

Anti-Bacterial Agents↗
PubMed1980

Gonorrhea in Rotterdam caused by penicillinase-producing gonococci.

Recently the prevalence of strains of penicillinase-producing Neisseria gonorrhoeae (PPNG) in the Netherlands has increased. A study of demographic and clinical data from patients with gonorrhoea due to PPNG strains, and of the biological characteristics of the strains isolated in Rotterdam, show that Asian strains of PPNG seem at present to be endemic in the Netherlands. Spectinomycin was found to be effective in the treatment of patients with gonorrhoeea due to PPNG strains.

Female↗
PubMed1980

Antibiotic sensitivities of gonococci isolated in Rotterdam and results of treatment with cefuroxime.

In a study of the efficacy of cefuroxime in the treatment of 278 cases of acute gonorrhoea (including rectal and pharyngeal infections and infections due to penicillinase-producing Neisseria gonorrhoeae), high cure rates were found for both male (98.8%) and female patients (98.6%). Disadvantages of this treatment are its high cost and the relatively high proportion of patients who develop postgonococcal urethritis.

Anti-Bacterial Agents↗