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Biomedical subjects

E Stark

Publications and source records attributed to E Stark.

173 records · Page 10Linked to original sources
PubMedDate not supplied

Corticotroph cells in primary cultures of rat adenohypophysis: a light and electron microscopic immunocytochemical study.

Monolayer cultures of trypsin-dispersed cells of the rat adenohypophysis were grown for 5 to 54 days. ACTH was localized by immunocytochemistry using an antiserum to synthetic ACTH1-28 prepared in rabbit and sheep anti-goat immunoglobulin coupled with peroxidase. ACTH content of the culture medium was measured by radioimmunoassay. Corttion time. The corticotrophs retained their essential morphological characteristics. Immunological staining was found in the secretory granules, some tubular or saccular structures, parts of the rough endoplasmic reticulum, and the cytoplasmic matrix. Immature secretory granules in the Golgi apparatus as well as some Golgi elements showed different degrees of immunoreactivity. In agreement with the high ACTH content of the culture medium the number, size and shape of the secretory granules, the active Golgi apparatus, the high amount of extragranular ACTH as well as pictures suggesting granule extrusion claim for a high ACTH synthesis and transport (and low ACTH storage) in the cultured corticotrophs.

Adrenocorticotropic Hormone↗
PubMedDate not supplied

Catecholamine turnover measurement and ACTH-induced short-term changes of catecholamine levels in individual brain nuclei.

The effect of acute ACTH treatment on catecholamine content of various brain nuclei was followed by radioenzymatic assay. The hormonal treatment employed affected catecholamine levels only in some hypothalamic nuclei and in the locus coeruleus. Some effects could be detected as rapidly as 10 min after the injection of the hormone. Employing alpha-methyl-para-tyrosine, catecholamine turnover in individual nuclei was estimated, and shown to be quite variable from one area to the other. However, severe limitations seem to exist in vivo, which restrict the feasibility of this approach to basal or chronic situations.

Adrenocorticotropic Hormone↗
PubMedDate not supplied

Preparation procedure for cerebrospinal fluid that yields cytologic samples suitable for all types of staining, including immunologic and enzymatic methods.

A new method for the cytologic investigation of the cerebrospinal fluid is described. Preparation of the cells is done by successive centrifugation, resuspension of the cells in a protein-enriched medium and cytocentrifugation. The method provided both good cell yields and morphologic preservation. All stainings, including enzymecytochemical and immunocytochemical, could be performed on the specimens. The cell-free fluid from the first centrifugation is also available for use in other studies.

Cell Separation↗
PubMedDate not supplied

Catalepsy, hypermotility, increase of striatal acetylcholine release induced by morphine and Met-enkephalin as affected by prolonged hydrocortisone and ACTH treatment.

In the rats treated with ACTH or hydrocortisone for 14 days the catalepsy induced by morphine was almost completely inhibited, while the haloperidol induced catalepsy remained unchanged. The morphine induced hypermotility was altered neither by prolonged treatment with ACTH nor by an acute glucocorticoid administration. Inhibition of Na/K ATPase by ouabain led to an increase of striatal acetylcholine (Ach) release, which was enhanced by Met-enkephalin. This effect of the opioid peptide was not demonstrable in the striata of ACTH or hydrocortisone pretreated rats. It is concluded that glucocorticoids are regulatory factors of the striatal opiate neurotransmission possibly via altered receptorial mechanisms.

Adrenocorticotropic Hormone↗