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Biomedical subjects

E Soppi

Publications and source records attributed to E Soppi.

96 records · Page 6Linked to original sources
PubMed1977

Two subpopulations of guinea-pig thymocytes with different maturation stages.

Two subpopulations (F4 and F6) of guinea-pig thymocytes were separated by using bovine serum albumin gradient centrifugation. The majority of F4 thymocytes were weakly alkaline phosphatase (AP) positive cells, while most of F6 thymocytes were strongly AP positive. The significant difference between their AP activities was confirmed biochemically. In the ultracytochemical study the majority of unfractionated and F6 thymocytes were light and AP positive, whereas F4 thymocytes were mostly dark, weakly AP positive cells. F4 thymocytes responded well to PHA and Con A, while F6 thymocytes failed to respond to these mitogens. The subpopulations did not differ in their homing properties. These findings indicate that strongly AP-positive cells are immature thymocytes and weakly AP-positive cells represent a more mature subpopulation of thymocytes. A hypothetical scheme for differentiation of guinea-pig thymocytes is presented.

Alkaline Phosphatase↗
PubMed1976

Biological rhythm of cell-mediated immunity in man.

The biological rhythm of the spontaneous lymphocyte transformation and the lymphocyte response to phytohaemagglutinin (PHA), concanavalin A (Con A) and purified protein derivative of tuberculin (PPD) was studied in six healthy male volunteers. The count of peripheral blood lymphocytes was increasing from 08.00 until 02.00 hr. The level of plasma cortisol was highest at 08.00 hr and lowest at 20.00 hr. The spontaneous lymphocyte transformation showed a biphasic cycle during the day; the peak values were observed at 08.00 and 20.00 hr and a significant depression was found at 14.00 hr. The response of peripheral blood lymphocytes to PHA and Con A varied inversely with the level of plasma cortisol. The PPD response was lowest at 08.00 hr, but the highest value was observed at 02.00 hr. No significant day to day variation was found in any of the parameters studied.

Adult↗
PubMed1975

A new micromethod for lymphocyte stimulation using whole blood.

A new micromethod for stimulation of human lymphocytes is described. The use of small amounts of whole blood, microtiter plates, 125I-5-iodo-2'-deoxyuridine, 5-fluoro-2'-deoxyuridine and a multiple cell-culture harvester makes the method easy, rapid, reproducible and suitable for routine laboratory use.

Blood↗