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Biomedical subjects

E Soppi

Publications and source records attributed to E Soppi.

At least 73 records · Page 4Linked to original sources
PubMed1987

Immunoblotting analysis of human IgM, IgG and IgA response to chromosomally coded antigens of Yersinia enterocolitica 0:3.

Human antibody response after Yersinia enterocolitica infection was studied by immunoblotting sequentially collected sera against a whole-cell homogenate of Y. enterocolitica serotype 0:3, grown under conditions restrictive for the plasmid. The antibodies observed were directed against a multitude of chromosomally coded antigens, and a considerable individual heterogeneity was found in the reactions of individual sera. The early (0-2 months) and late (greater than or equal to 11 months) responses were directed against the same antigenic determinants. Antibodies against different bacterial epitopes decreased evenly with time, indicating that several, if not all, antigenic epitopes of the bacteria are responsible for the prolonged antibody production. IgM responses by most patients declined within a few months but were surprisingly strong in some even one year after onset of the infection. IgG antibodies showed a strong reaction against a region corresponding to lipid A and core of the bacterial LPS, whereas IgM and IgA recognized this region less often. No other significant differences between IgM, IgG and IgA responses were observed. Immunoblotting of sera from patients with post-infection complications (arthritis, iritis, erythema nodosum) did not reveal any additional or specifically involved antigens. Altogether, these findings suggest that Yersiniae causing the original infection may hide in some of the patients for prolonged periods.

Antibodies, Bacterial↗
PubMed1987

Serum secretory IgA, IgA1 and IgA2 subclasses in inflammatory bowel and chronic liver diseases.

Healthy controls and patients with autoimmune chronic active hepatitis (CAH), primary biliary cirrhosis (PBC), coeliac disease (GSE), Crohn's disease (CD) and ulcerative colitis (UC) were examined for serum immunoglobulin A (IgA), secretory IgA (sIgA) and subclasses IgA1 and IgA2 concentrations separately. Elevated secretory IgA levels are found in CAH and PBC. IgA2 level as well as the proportional part of IgA2 out of the serum total IgA are significantly increased in GSE and in CD, while in UC IgA1 levels are significantly decreased as compared to controls. Serial determinations of IgA subclasses may provide a differential diagnostic tool in inflammatory bowel diseases and the findings support the view that increased sIgA levels in CAH and PBC probably have a different origin.

Adult↗
PubMed1986

Unusual association of hyperkalemia and hypertension.

We report an unusual association of hyperkalemia, mild hyperchloremic acidosis, and hypertension in a young woman. Pseudohyperkalemia, Addison's disease, renal insufficiency, classical hyporeninemic hypoaldosteronism, isolated hypoaldosteronism, and iatrogenic causes were excluded. The patient's findings were compatible with a rare syndrome designated as type II pseudohypoaldosteronism, Gordon's syndrome.

Adult↗
PubMed1985

Immune functions in Parkinson's disease lymphocyte subsets, concanavalin A-induced suppressor cell activity and in vitro immunoglobulin production.

Immune abnormalities, including the occurrence of autoantibodies against neural structures, diminished numbers of peripheral blood lymphocytes and reduced mitogen responses have been observed in patients with Parkinson's disease. In this study, we found that among the peripheral blood mononuclear cells (PBMNC) of patients with Parkinson's disease, there is a reduced number of cells spontaneously secreting immunoglobulins (Ig). After pokeweed mitogen stimulation, PBMNCs from patients with advanced Parkinson's disease had decreased capacity to elaborate Igs in vitro. In addition, there was evidence suggesting that OKT4+ (helper/inducer) subset may be decreased in Parkinson's disease along with disordered concanavalin A-induced suppressor cell activity. The findings do not primarily suggest an autoimmune pathogenesis of Parkinson's disease. The immune changes now observed partially resemble those seen in normal ageing, though being quantitatively exaggerated in Parkinson's disease. It is suggested that immune abnormalities in Parkinson's disease may result from disordered neural-immune regulation.

Aged↗
PubMed1985

Concanavalin A induced and spontaneous suppressor cell capacity of newborn and fetal lamb lymphocytes.

Concanavalin A induced newborn lamb blood lymphocytes suppress the proliferation of adult lymphocytes to the same magnitude as similarly induced adult suppressor cells. Concanavalin A induced suppressor capacity of fetal spleen cells at term is not as strong as that of newborn peripheral blood lymphocytes. Thymocytes of the same fetuses do not possess any suppressive capacity inducible by this mitogen, but show a spontaneous suppressor capability. Spleen cells exhibit only a low spontaneous activity. Interestingly, when peripheral blood lymphocytes of newborn lambs spontaneously suppress the response of both unrelated and maternal cells, adult lymphocytes enhance the proliferation. These results show that functional suppressor cells are found in both newborn and fetal lambs. Our data also indicate that cells spontaneously suppressive are established in thymus and spleen, whereas cells that can be rendered suppressive by concanavalin A stimulation are found in the spleen. Therefore, the spontaneous thymic suppressor cells may represent a more primitive cell population than those of the spleen.

Animals↗
PubMed1985

Evidence against HLA and immunological dependence of disease outbreak in SLE. Immunological characterisation of identical twins clinically discordant for SLE.

Identical female twins clinically discordant for 20 years for SLE were studied. Their HLA-haplotype was A1,28; B8w6,w35; Cw3,w7; Dr3,4. Both twins had a raised erythrocyte sedimentation rate, autoantibodies, and circulating immune complexes. The diseases sibling had a reversed OKT4/OKT8 ratio (0.43), decreased helper T cell number, defective pokeweed mitogen (PWM) induced plasma cell differentiation, and overactive hydrocortisone sensitive suppressor cells. Immunological abnormalities may be only partly HLA related (B8; Dr3), but are most probably secondary to the disease process in the sibling with SLE. Exogenous and/or endogenous factor(s) other than genetic or immunological are suggested as being operative in the predisposition to and expression of SLE.

Adult↗
PubMed1985

Thymoma with immunodeficiency (Good's syndrome) associated with myasthenia gravis and benign IgG gammopathy.

We treated a case of thymoma with immunodeficiency (Good's syndrome) associated with a rare combination of other parathymic syndromes including myasthenia gravis, benign IgG lambda M component, pernicious anemia, and diabetes. The characterization of the patient's immunologic capacity disclosed practically normal T-cell number and mitogenic responses but impaired lymphokine production as well as B-cell function.

Humans↗
PubMed1984

Effect of cardiopulmonary resuscitation-induced stress on cell-mediated immunity.

Functions of cell-mediated immunity were studied from 11 patients after cardiovascular resuscitation and from matched controls who were simultaneously under observation. The resuscitated patients were anergic to recall skin antigens (93% negative) as compared to the controls (62%) (p less than 0.01). The anergic state correlated with the outcome of the patients. Lymphocyte numbers did not differ between these groups, but the number of T cells was significantly decreased, and B cells and granulocytes was increased in resuscitated patients. Lymphocytes from resuscitated patients responded to mitogenic stimulation although the responses were lower than those of the controls. Decreased lymphocyte responses were partly due to serum factor(s) which were not attributable to serum cortisol concentration. In addition the findings favour a change in the compartmentalization of lymphocyte subsets resulting in increased number of suppressor cells and/or increased sensitivity of lymphocytes to suppressive humoral factor(s) in the circulation. The anergy in skin evidently represents the final outcome of the dysfunction of several arms of cell mediated immunity.

Aged↗
PubMed1984

Selective effect of different retinoids on the primary antibody response in normal chickens.

The effect of three retinoids--retinoic acid, etretinate and trimethylmethoxyphenyl(TMMP)-retinoic acid--was studied on mitogen-induced lymphocyte transformation and on primary antibody response against Brucella abortus and bovine serum albumin (BSA). None of the drugs had an effect on lymphocyte transformation. Antibodies of IgG and IgM class were measured with a modified enzyme immunoassay, able to estimate separately the amounts of high avidity and total specific antibodies. No effect was seen on antibodies against B.abortus, a T-independent antigen, but was observed on antibodies against BSA, a T-dependent antigen. Retinoic acid increased primarily IgM anti-BSA antibodies, while TMMP-retinoic acid increased high avidity IgG anti-BSA antibodies. Etretinate had no influence on antibody production. Retinoic acid and TMMP-retinoic acid are able to modulate T-dependent humoral immune response. This selective effect of retinoic acid and TMMP-retinoic acid on T-cells makes them promising tools in the regulation of humoral immune response.

Animals↗
PubMed1984

Acute effect of exogenous thyroxine dose on serum thyroxine and thyrotrophin levels in treated hypothyroid patients.

Acute effects of exogenous thyroxine on the serum thyroxine (T4), free thyroxine (FT4) and thyrotrophin (TSH) levels were studied in 20 hypothyroid women during the long-term maintenance therapy. All patients were euthyroid by clinical and laboratory parameters (T4). In nine patients TSH secretion was totally suppressed (less than 1 mu/l) by the mean dose of 178 micrograms thyroxine. Eleven patients had TSH values greater than 1 mu/l. Their mean thyroxine dose (132 micrograms) was not significantly lower than that which totally suppressed the TSH secretion. Ingestion of thyroxine resulted in maximal T4 and FT4 levels at 2 h after taking the drug. Simultaneously TSH concentration reached its minimum. The results indicate that it is necessary to know when the patient has taken the thyroxine in relation to determination of T4, FT4 and TSH levels in order to make the right decisions about the substitution dose of thyroxine. Thus it is essential to take blood sample for hormone determination before the patient ingests the daily thyroxine dose. However, among hypothyroid patients substituted by thyroxine there are individuals whose TSH secretion is not suppressed by normal T4 and FT4 levels.

Adult↗
PubMed1982

Effects of indomethacin on lymphocyte proliferation, suppressor cell function, and leukocyte migration inhibitory factor (lmif) production.

The in vitro effect of indomethacin (IND) on PHA- and Con-A-induced immune responses was studied. The results showed that the enhancement of both PHA and Con A responses by IND does not significantly depend on the concentrations of mitogens or IND. A significant negative correlation was found between the initial PHA response and augmentation index, whereas there was a positive correlation between the initial Con A response and the enhancement of the Con A responses. Indomethacin significantly increased the Con-A-induced suppressor cell activity and PHA-induced leukocyte migration inhibitory factor production (LMIF), whereas the effects on the PHA-induced suppressor cell activity and Con-A-induced LMIF production were not significant. The findings indicate the indomethacin has a dual effect on PHA- and Con-A-induced immune responses probably reflecting intrinsic differences among T cells, for example, distinct lymphokines produced by different T-cell subpopulations.

Cells, Cultured↗
PubMed1982

Differential in vitro effects of etretinate and retinoic acid on the PHA and con A induced lymphocyte transformation, suppressor cell induction and leukocyte migration inhibitory factor (LMIF) production.

The in vitro influence of two retinoids, etretinate and retinoic acid, on the human lymphocyte transformation, on the induction of suppressor cells and on leukocyte migration inhibitory factor (LMIF) production were investigated. Nontoxic concentration of retinoic acid increased significantly the PHA response to suboptimal mitogen concentrations, but had no effect on the Con A response; it also abolished the PHA induced LMIF production. In corresponding assays etretinate was without effect. Etretinate augmented the PHA induced suppressor cell activity, while retinoic acid was ineffective. No effect was observed on Con A induced suppressor cells by either retinoid. The findings extend the information about the immunomodulatory effects of retinoids and demonstrate that retinoic acid and etretinate have different effects on PHA and Con A induced immune responses. The mode of action of retinoids is discussed.

Concanavalin A↗
PubMed1982

Cell-mediated immunity in untreated and PUVA treated atopic dermatitis.

In 22 adult patients with atopic dermatitis, lymphocyte subpopulation counts, mitogenic responses to several PHA and Con A concentrations, nonspecific mitogen induced, and indomethacin sensitive suppressor cell functions and leukocyte migration inhibitory factor production were investigated. Patients with severe atopic dermatitis had normal PHA and Con A responses, although somewhat lower than matched controls, while mild AD patients equalled controls in this respect. No significant differences between AD patients and controls were detected with respect to lymphocyte subpopulations, suppressor cell function or leukocyte migration inhibitory factor production. Methoxalen plus ultraviolet light (PUVA) therapy induced significant clinical improvement in 9 out of 10 treated patients. A concomitant decrease of the mitogenic responses and increase of the Con A induced nonspecific suppressor cell activity was recorded, while other immunological parameters remained unaffected by the therapy. Thus PUVA therapy induces both local clinical and systemic immunological manifestations. The possibility that the observed immunological changes would be operative in the PUVA induced healing process, however, seems unlikely, as the present study did not disclose any obvious relationship between immune parameters and severity of atopic dermatitis.

Adult↗