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E Sercarz

Publications and source records attributed to E Sercarz.

62 records · Page 4Linked to original sources
PubMed1969

The X-Y-Z scheme of immunocyte maturation. VII. Cell division and the establishment of short-term IgM memory.

Short-term IgM memory is established within 1 day after primary injection. This 1 day priming is independent of cell division as it can take place in the pressence of methotrexate or hydroxyurea. The primary response involves a similar 1 day nonproliferative phase. On the contrary, cells responding to a second injection of antigen after short priming intervals begin to proliferate within hours. This implies a qualitative difference between the antigen-sensitive cells in primed and normal animals. After 1 day, further proliferative expansion of memory can occur, which is probably antigen dependent. The response to several dose regimens indicates that repeated antigenic contact is needed to maintain the IgM response.

Animals↗
PubMed1968

High-dose delay of the immune response. Effect of actinomycin D on continuation of the immune response in vitro.

The continuation of the primary and secondary antibody response to human serum albumin (HSA), induced in vivo, was followed in explanted chicken spleen fragments. The effect of actinomycin D (AMD) on the in vitro response was studied in spleens from chickens injected with various doses of HSA and removed at differing intervals after injection. The antibody response of "early spleen" cultures was AMD-sensitive, while cultures of spleens removed later were AMD-resistant. It was suggested that this shift represented the development of cells with in vivo preformed RNA involved in specific immunoglobulin synthesis. With increasing doses of HSA, the AMD-sensitive phase was prolonged, suggesting the delay of mRNA formation or some other AMD-inhibitable process in vivo. With large doses of HSA, the immune response in vitro was decreased, starting after a 1-2 day delay and not occurring in the presence of AMD. Massive doses of HSA completely inhibited the continuation of the response in vitro by spleen fragments removed between the 2nd and 5th day after injection. The results point to the controlling role of antigen dose in determining the onset of macromolecular synthesis during immunocyte maturation.

Animals↗
PubMedDate not supplied

The steering function of T cells in expression of the antibody repertoire directed against multideterminant protein antigen.

Limitation in the T-cell repertoire has the consequence of reducing the heterogeneity of antibody production. This has been demonstrated in three different systems, in which the T-cell restriction was imposed (1) by tolerance, (2) by peptide priming (3) by natural scarcity. Therefore, expressed repertoires may represent only a portion of the genetically prescribed V regions.

Animals↗