Search PubMed⌕ Search

Biomedical subjects

E Seifert

Publications and source records attributed to E Seifert.

At least 91 records · Page 5Linked to original sources
PubMed1988

[Colorectal carcinoma--diagnostic requirements from the clinical point of view].

More than 90% of colorectal cancers develop from adenomas (adenoma-cancer-sequence). An adequate preoperative diagnosis must include the tumour classification: 1. Typing = identification of the tumour type (by histology), 2. Grading = degree of differentiation and malignancy (by histology), 3. Staging = anatomical spreading of the tumour (preoperative: clinically, postoperative: following the TNM classification). Therefore, an exact preoperative tumour classification needs an endoscopic and histologic examination. Radiology does not fulfil these criteria and remains a complementary method only. In adenomas with severe cell atypia and in adenomas with invasive cancer with few exceptions (degree of malignancy 3, signet ring cell carcinoma, undifferentiated cancer) polypectomy (by snare or surgically) is the method of choice. Only when the excision cannot be performed totally or a lymphatic infiltration is proven radical operation has to be done subsequently. In cases of advanced colorectal cancer only total colonoscopy with forceps-biopsies ( = typing, grading) and clinical staging with ultrasonography (liver metastases) is essential. Examination of the entire colon is decisive for preoperative diagnosis (metachronous cancer in 1,6%, additional premalignant lesions up to 20%).

Colonic Neoplasms↗
PubMed1987

[Are stomach polyps an indicator of colonic carcinoma and colonic polyps an indicator of stomach carcinoma?].

Over a period of six years a total of 407 patients with polyps of the gastrointestinal tract were examined by gastroscopy and coloscopy and the findings analysed retrospectively. Among patients with colon polyps 10.5% were found also to have polypoid gastric lesions, among those with adenoma of the colon the prevalence was 11.7%. Only 2.4% of simultaneously diagnosed gastric lesions were found to be malignant or premalignant, a figure similar to the population average. But in patients with more than ten polyps of the colon both the prevalence of polypoid gastric changes and the significance of polyps with respect to precancerous lesions were clearly increased. On the other hand, in patients with epithelial polyps and/or glandular cysts colon polyps were found in 45%, in 42% with precancerous changes (adenoma). Thus patients with epithelial gastric polyps and glandular cysts probably constitute a group with a real additional risk of colon carcinoma. Regular coloscopy will thus reveal precancerous changes (adenoma) in the colon of 42% of such patients; coloscopic polypectomy will be an effective prophylactic measure against carcinoma of the colon.

Adenomatous Polyposis Coli↗
PubMed1987

Analysis of Krüppel protein distribution during early Drosophila development reveals posttranscriptional regulation.

We have examined the spatial and temporal patterns of expression of the Krüppel (Kr) protein, a gap gene product, during Drosophila embryogenesis. Antibodies directed against the Kr protein revealed patterns of nuclear staining that represent subpatterns of Kr transcript accumulation in particular tissues. This indicates that the distribution of Kr protein is not a direct response to Kr mRNA accumulation, and that Kr protein expression requires a second level of control in addition to spatially regulated transcription. Our data provide evidence for posttranscriptional control that may involve an intron present in the 5' region of one of the two Kr transcripts. The intron-containing transcript is the only potential source of new Kr protein synthesis after gastrulation. The finding of late and transient patterns of Kr activity in several tissues, such as the developing nervous system, amnion serosa, and muscle precursor cells suggests that Kr activity may be required in several developmental processes after segmentation has been completed.

Animals↗
PubMed1987

Somatostatinoma of Vater's papilla and of the minor papilla.

Two cases of somatostatinomas of the major and minor papilla are reported. The tumors were characterized by solid trabecular and tubular arrangements of tumor cells and in one case by the presence of microvilli and psammoma bodies. The tumor cells contain many amine precursor uptake and dicarboxylation (APUD)-granules with a diameter of 300 to 500 nm and low electron density. Immunoperoxidase stain showed intense staining only for somatostatin and low density of neuron-specific enolase. Those tumors either resulted from pluripotent endocrine cells or from D-cells of the mucosa or glands of the papilla.

APUD Cells↗
PubMed1987

The gooseberry-zipper region of Drosophila: five genes encode different spatially restricted transcripts in the embryo.

Genetic analysis of the Drosophila chromosome region 60 E9-F1 identified two functions affecting embryonic development; gooseberry (gsb), a segment polarity gene, and zipper (zip), an unclassified gene which affects cuticle formation severely. By contrast, molecular analysis revealed five genes with different temporal and spatial patterns of expression in the embryo. Candidate genes for gsb and zip functions were identified. Two adjacent genes are eventually expressed in regular stripes within the posterior region of each segment. One of them is expressed initially in a pair-rule mode; the second gene expresses reduced levels of transcripts in a mutant which leaves the transcribed region and the sequences up to the second gene intact. This observation, the patterns of transcripts in the embryo and the genetic data suggest that both genes are involved in gooseberry segmentation function. zip is expressed in neural tissue and not in epidermal anlagen. Embryos lacking zip activity also develop abnormal neural tissue consistent with the argument that the zip cuticle phenotype is a secondary effect. Additional newly identified genes are expressed in specific domains of the embryo, covering mesoderm anlagen and the dorsal region of embryos at blastoderm stage, respectively.

Journal Article↗
PubMed1986

A conserved family of nuclear proteins containing structural elements of the finger protein encoded by Krüppel, a Drosophila segmentation gene.

Krüppel (Kr), a segmentation gene of Drosophila, encodes a protein sharing structural features of the DNA-binding "finger motif" of TFIIIA, a Xenopus transcription factor. Low-stringency hybridization of the Kr finger coding sequence revealed multiple copies of homologous DNA sequences in the genomes of Drosophila and other eukaryotes. Molecular analysis of one Kr-homologous DNA clone identified a developmentally regulated gene. Its product, a finger protein, relates to Kr by the invariant positioning of crucial amino acid residues within the finger repeats and by a stretch of seven amino acids connecting the finger loops, the "H/C link." This H/C link is conserved in several nuclear and chromosome-associated proteins of Drosophila and other eukaryotic organisms including mammals. Our results demonstrate a new subfamily of evolutionarily conserved nuclear and possibly DNA-binding proteins that again relate to a Drosophila segmentation gene as in the case of the homeo domain.

Animals↗
PubMed1986

How to run an endoscopy unit? Experience in the Federal Republic of Germany. Results of a survey of 31 centers.

A survey of 31 German hospitals (14 university hospitals with a special gastrointestinal service, 13 key hospitals with specialized GI units, mainly university teaching hospitals, and 4 smaller hospitals without specialization) showed that a service providing the usual gastrointestinal endoscopic procedures is available in all university and key hospitals. In the university hospitals, an average of 4,765 procedures per year are performed, in the key hospitals with specialized GI units 5,558, and in the smaller hospitals without specialization 2,935. Such special methods as ERCP, PTC and operative endoscopy are usually restricted to special clinics. The number of doctors and nursing staff is adequate at university hospitals and in specialized key hospitals. Smaller hospitals without specialization need more nursing staff. All hospitals need more rooms. The supply of instruments is sufficient at university and key hospitals, but more instruments are needed in general non-specialized hospitals. German endoscopic units are relatively well equipped with X-ray machines, but further X-ray equipment is still needed to achieve less dependence on centralized radiological departments. There is a distinct need for more waiting and recovery rooms. At the present time, there is no optimal endoscopic unit in Germany. Better and appropriate outfitting of the units, and an optimized floor-plan encompassing endoscopy rooms, rooms for preparation and after-care are needed--which is not practical in the case of existing hospitals, but must be considered when planning new hospitals.

Endoscopes↗
PubMed1985

An early role of maternal mRNA in establishing the dorsoventral pattern in pelle mutant Drosophila embryos.

Mutations of the maternal effect locus pelle (pll) cause dorsalized Drosophila embryos. In extreme mutants, the embryo develops into a long hollow tube of dorsal cuticular structures with no sign of ventral pattern elements. Injection of wild-type cytoplasm or poly(A)+RNA into mutant pll embryos partially restores the normal pattern. Rescuing activity is present in the wild-type cytoplasm until the late blastoderm stage, but is already absent from the poly(A)+RNA fraction by the time of pole cell formation. At the same time, pll embryos fail to respond to injected biologically active poly(A)+RNA. This indicates that pll+ mRNA is lost early from the pool of maternal RNA and that there is a non-RNA component of rescue. This component, most likely the pll+ protein, appears to be unequally distributed in wild-type embryos.

Animals↗
PubMed1985

Famotidine versus ranitidine for the short-term treatment of duodenal ulcer.

One-hundred and eight-three patients with endoscopically proven duodenal ulcers, enrolled in this prospective, double-blind study, were randomly allocated to receive famotidine 40 mg once at night, 20 mg twice daily, 40 mg twice daily, or ranitidine 150 mg twice daily for 2-8 weeks. Pretreatment characteristics between the four groups were similar. After 4 weeks of treatment, among the famotidine-treated patients, 38 of 42 (90.5%) healed with the 40 mg once nightly regimen, 35 of 42 (83.3%) with 20 mg twice daily, and 37 of 41 (90.2%) with 40 mg twice daily. In the ranitidine group 40 of 43 patients (93.0%) healed. After 8 weeks of treatment, the respective data were: 97.6, 95.2, 100 and 93.0%. Different results between the famotidine groups and the ranitidine group were not statistically significant. All treatments were well tolerated and severe adverse events were rare. Famotidine 40 mg given once at night appears to be as safe and effective as conventional therapy with ranitidine, indicating the importance of overnight gastric acidity in the pathogenesis of duodenal ulcer disease.

Adolescent↗
PubMed1985

[Famotidine versus ranitidine in the acute treatment of duodenal ulcer. A multicenter comparative study in Germany].

185 patients with endoscopically proven duodenal ulcers were randomly allocated to treatment with either famotidine 40 mg nocte, 20 mg bid, 40 mg bid or ranitidine 150 mg bid for 2-8 weeks in a prospective double-blind study. The four groups were similar with regard to age, sex, duration of ulcer disease, smoking habits etc. After 2 weeks treatment 28/42 patients (66.7%) healed on famotidine 40 mg nocte, 24/42 patients (57.1%) on famotidine 20 mg bid, 26/41 patients (63.4%) on famotidine 40 mg bid and 28/43 patients (65,1%) on ranitidine 150 mg bid. The corresponding healing rates after 4 weeks were 90.5%, 83.3%, 90.2% and 93%, respectively. After 8 weeks more than 93% of the patients had healed ulcers. At each time there was no statistical difference between the different famotidine regimens and the ranitidine group. All treatments were well tolerated and severe adverse events were rare. Famotidine 40 mg at night, therefore, appears to be as good as conventional ranitidine.

Adolescent↗
PubMed1985

[Lipid islands in the esophagus].

Lipid islets (xanthomas, xanthelasmas) occur rather frequently in gastric mucosa; such islets have been described in the esophagus just recently. We have observed such a patient. As has been the case in the first patient described, the islets were located in the middle part of the esophagus and appeared during endoscopy as flat, yellowish elevations. On biopsy, foam-cell macrophages were found in the tunica propria of the esophageal mucosa. Pathogenesis of the lipid islets in our patient could be caused by a disturbance of carbohydrate and fat metabolism.

Biopsy↗
PubMed1985

[Duodenal stenosis in coincidence with annular pancreas and cancer of the papilla].

We report on a case of annular pancreas complicated by the development of a carcinoma of the papilla causing secondary chronic obstructive pancreatitis. Eventually, a high-grade stenosis of the duodenum developed, leading to symptoms of gastric outlet obstruction. The stenosis was proved by endoscopic and radiologic diagnosis, but the exact anatomical condition was only revealed by an operation. Efficient diagnostic and therapeutic procedures in annular pancreas with its potential complications are discussed in detail.

Adenocarcinoma, Papillary↗