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E Schulz

Publications and source records attributed to E Schulz.

At least 271 records · Page 15Linked to original sources
PubMed1976

[Quantitative study of primitive pyramidal cells in rat anterior cingular cortex].

In the present paper the primitive pyramidal cells of the Vthlayer of the anterior cingulate cortex in adult male white rats were analyzed quantitatively and compared statisticaly with large pyramidal cells of the same region. The number of dendrites, the total lengths of dendrites, the number of spines and the density of spines -- according to the order of dendrites -- show similarity between the primitive pyramidal cells and the large pyramidal cells the latter one exhibit the higher values. The curves of distribution of the various density of spines along the apical main dendrite of both cell types are similar in shape, too. The lengths of the dendritic fields and their basal spines-values are without significant distinction for both cell types, however there are more dendritic fields in large pyramidal cells. Refered to a complete pyramidal neuron they can say: there are significantly higher values in large pyramidal cells for the number of dendrites and their total lengths, the total number of spines, the number of branching sites sites and free endings. However the density of spines of the complete neuron has no significant differences between primitive and large pyramidal cells.

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PubMed1976

[Quantitative study of dendritic tree of large (regular) pyramidal cells of lamina V in rat anterior cingular cortex].

At three month old male rats the dendritic trees of 36 large pyramidal cells in the Vth layer of the anterior cingulate cortex were analyzed quantitatively by means of the Golgi-Kopsch method. 12 pyramidal cells were localized at the medial border of the regio praecentralis (neocortex), 12 cells were localized in the adjacent mesoarchicortex, 12 cells were localized in the mesoarchicortex, which are three subregions of the anterior cingulate cortex. By means of a varianz-analysis the values of the three subregions were compared, in order to verify a supposed gradient of differentiation. 1. In the three subregions basal the number and the branching behaviour of the dendrites are greater than apical. The pyramidal cells of the neocortex and of the mesoneocortex have significantly more basal dendrites of the 1st, 2nd, 3rd, and 4th order than those of the mesoarchocortex. There are significantly more apical dendrites of the 1st and 2nd order at pyramidal cells of the neocortex compared with the two other subregions. 2. The total lengths of the dendritic branches are in proportion to the dendritic numbers of the corresponding orders. There are following tendencies for the three subregions: the number and length of apical dendrites decrease with the increasing number of order, basally, however, the number and length first increase and than decrease. 3. The total length of all basal dendrites of a pyramidal cell is largest in the mesoneocortex, than follows the neocortex, least values they find in the mesoarchicortex. There are significant differences between every one of the three subregions. The values of the total dendritic tree of a pyramidal cell (total dendritic length, total number of dendrites, volume of the dendritic tree, number of branching sites and free endings, total apical dendritic length) show significant differences between neocortex and mesoarchicortex as well as between mesoneocortex and mesoarchicortex. There are no significant differences between noecortex and mesoneocortex. The values of the total dendritic tree demonstrate that neocortex and mesoneocortex have a similar organization, while the lamina V-pyramidal cells of the mesoarchicortex indicate a significantly smaller and less branched dendritic tree.

Animals↗
PubMed1976

Mechanism of 67Ga accumulation in normal rat liver lysosomes.

67Ga accumulates in various malignant tumors and parenchymatous tissues. It was found to be associated with the soluble fraction of lysosomes (11). The present work investigates the mechanism of 67Ga accumulation in normal liver cells. Lysosomes were isolated from rat liver after intravenous injection of carrier free 67Ga. The soluble lysosomal fraction was obtained by sonication followed by centrifugation at 105,000 xg for 2 hrs. Gel filtration on Sephadex G 25 superfine was carried out on the soluble lysosomal fraction in order to investigate the stability of the 67Ga-protein complex within the lysosomes under EDTA treatment. After treatment with 1 mM/l EDTA a considerable amount of the protein bound radioactivity was found to be liberated. In further experiments the 67Ga binding lysosomal proteins were fractionated by electrophoresis on 7% polyacrylamide gels (0.5 cm x 5.5 cm). After staining with Coomassie blue 18 separated protein bands were apparent. 67Ga distribution within the gels was assessed by direct counting of radioactivity in gel slices. A considerable amount of the intralysosomal protein bound radioactivity migrated with a relative mobility of 0.36 corresponding to a protein band of molecular weight 85,000--90,000. This peak corresponded to the peak of 67Ga-labelled purified transferrin in control gels. These data were confirmed by immunoelectrophoresis combined with autoradiography: within the soluble lysosomal fraction a slight transferrin line could be identified. We conclude that 67Ga which is transported in the blood by transferrin (23) and taken up by the hepatic cell through endocytosis (32) is accumulated in the lysosomes associated with transferrin and its degraded fragments.

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