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Biomedical subjects

E Schauenstein

Publications and source records attributed to E Schauenstein.

At least 73 records · Page 4Linked to original sources
PubMed1976

[Citrate values in the whole blood of patients with malignant tumors of various locations].

Based on reports on elevated concentrations of 2-oxoglutarate in the blood of human subjects with tumors of various locations, data are presented on the concentration of citrate and pyruvate in the whole blood of 41 patients with malignant tumors (mainly of the female breast and of the digestive tract), 30 patients with benign surgical diseases and 12 healthy subjects. Adopting a level of significance of 2P=0.10 differences of concentration were found between the following collectives: (1) The mean value of citrate for the patients in tumor stage T4 was decreased by 23%, compared with healthy persons, and by 19%, compared with patients with benign surgical diseases. (2) The mean value of pyruvate for the patients with malignant tumors was increased by 40%, compared with healthy subjects and by 17%, compared with patients with benign surgical diseases. (3) The maximum of the pyruvate concentration was reached in tumor stage T3, where an increase of 52% was noted, compared with healthy subjects. (4) The pyruvate value of the patients with tumors of the digestive tract was increased by 34%, compared with healthy subjects. With regard to the total group of patients with malignant tumors, even with a low level of significance, no differences in citrate values were found as compared with the control groups. Thus the results do not indicate a disturbance of the citric acid cycle in the organism of tumor bearing hosts in general.

Adult↗
PubMed1976

[Microspectrometric quantification of 4-hydroxypentenal binding to protein thiols in Ehrlich ascites tumor cells].

After 30 minutes in 5.10(-3) M hydroxypentenal (HPE) the microscopically determinable proteinthiols (PSH) stained with DDD-Fastblue B decrease from 1,07 . 10(-14) to 0,61 . 10(-14) moles/cell. The loss of PSH of 0,46 . 10(-14) moles has a confidential range (99%) of +/- 0,12. As HPE reacts with thiols by an addition to the C3 = C2-group, the aldehydic group remains reactive and can be recognized intracellulary and quantitatively determined with 2,4-dinitrophenylhydrazine, whereby an uptake of 0,63 . 10(-14) moles of HPE per single cell is observed (confidential range +/- 0,11). The broad overlapping of the confidential ranges indicates clearly that the loss of PSH corresponds to the uptake of HPE. From the statistical distribution diagrams it can be seen that the PSH of practically all cells take part in the reaction, if however, to very different extent; the cells with high PSH concentrations being the most reactive ones.

Aldehydes↗
PubMed1975

[Relationship between Chemical Constitution and Theraeutic Activity of alpha, beta-unsaturated Aldehydes in the Ehrlich-Solid-Tumor of the Mouse].

Biochemical and biological effects and chemical properties of three alpha,beta-unsaturated aldehydes [acrolein (I), 4-hydroxypentenal (II), and 4-hydroxyundecenal (III)] were compared. These three substances inhibited the incorporation of -3H-thymidine in Ehrlich-ascites-tumor cells in vitro in the following order: I is greater than III is greater than II. For reactivities with glutathion in vitro the order was: I is greater than II is greater than III is greater than. We concluded there from that the high activity of I, as to its inhibition of thymidine incorporation and its toxicity, was related to its high activity against SH-groups. With respect to therapeutic effects and hydrophilia the following order: II is greater than I is greater than III was observed. In our opinion the high hydrophilia of II is responsible for the significant and good inhibition of tumour growth.

Acrolein↗
PubMed1975

[Effects of 4-hydroxypentenal on the noncancerous and the cancerous ectocervix uteri hominis (author's transl)].

PROBLEM: 4-hydroxy-2,3-transpentenal (HPE) represents the 5-C-homologue of a series of hitherto unknown alpha,beta-unsaturated aldehydes. The marked inhibition of DNA-biosynthesis by these chemical compounds, particularly by HPE, suggests blocking of mitosis by HPE due to direct action on the S-phase. It is known that DNA-biosynthesis as well as energy metabolism are generally more disturbed by HPE in animal tumor cells (Ehrlich ascites and solid tumors, NK/Ly sarcoma 37, sarcoma 180, plasmocytoma G, murine Harding-Passey melanoma) than in normal cells (liver, kidney, jejunum, spleen, thymus); for details see BICKIS et al. 1969; RINDLER et al. 1970; KAPFER et al. 1972). After systemic administration of HPE the concentration of the cytotoxic agent in the blood caysed a bituceabke but merely selective inhibition of the metabolism in experimental animal tumors; however, it was not sufficient to produce therapeutic results. The low stability of HPE in the blood and in the peritoneal cavity is to be attributed to its affinity to SH-groups. Therefore the biological tests had to be confined to the local administration of HPE. In this histological study of the action of HPE the substance was administered directly to the human portio vaginalis uteri (ecto- and endocervix) in cases of carcinomatous alterations, in cases of non-carcinomatous affections as well as in normal portions. MATERIAL AND METHODS: In this test 42 women were treated with HPE. 3 patients were observed clinically only, the other 39 cases were investigated also histologically. The histological studies were performed on the extirpated uterus (36 cases), the resected portio (1 case) and only on a conus (2 cases). In 17 of these patients examined histologically the portio was clinically free of pathological symptoms. They suffered from such more or less commonplace gynecological disorders as displacement of the uterus, incontinentia urinae relativa, uterus myomatosus, endometriosis and cystical adnexal tumors. 7 patients suffered from ectopies, 8 from Ca coli uteri, 7 were affected by invasive cancer of the cervix. 8 of the patients were older than 50 years, the others were aged between 25 and 50 years. Mode of HPE administration: 1. Application of a small HPE-soaked linen patch fixed on a Sta Seal impression of the vagina (Sta Seal=dental silicon impression paste). 2. Application by means of a small linen patch in a portio cap. The inner surface of a portio cap corresponding to the vaginal uterus in shape and size was coated with a circular linen patch. The unneeded segment was cut off. After moistening with the aldehyde (HPE) it was fit tightly to the inner surface of the cap and, in situ, to the portio surface as well. In several cases the portio did not fill the cap completely and did not contact the tissue in the region of the os uteri. Consequently, there the aldehyde effect was lower than in the lateral regions of the portio. Therefor 3...

Adult↗