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Biomedical subjects

E Santi

Publications and source records attributed to E Santi.

At least 19 recordsLinked to original sources

Bacteriophage lambda display of complex cDNA libraries: a new approach to functional genomics.

We describe the construction and characterization of two lambda surface displayed cDNA expression libraries derived from human brain and mouse embryo. cDNA inserts were obtained by tagged random-priming elongation of commercially available cDNA libraries and cloned into a novel lambda vector at the 3' end of the D capsid protein gene, which produced highly complex repertoires (1x10(8) and 2x10(7) phage). These libraries were affinity selected with a monoclonal antibody against the neural specific factor GAP-43 and with polyclonal antibodies that recognize the EMX1 and EMX2 homeoproteins. In both cases rapid identification of specific clones was achieved, which demonstrates the great potential of the lambda display system for generating affinity selectable cDNA libraries from complex genomes.

Amino Acid Sequence

Distraction osteogenesis to achieve mandibular vertical bone regeneration: a case report.

In this case report a surgical technique for vertical ridge augmentation is presented. The procedure, performed in a 30-year-old woman with an atrophied alveolar ridge in the anterior portion of the mandible, is based on the biologic concept of osteogenesis distraction previously introduced in orthopedic and maxillofacial surgery. After elevation of a full-thickness flap a horizontal osteotomy was performed 7 to 8 mm from the top of the ridge. Two vertical osteotomies were prepared with drills of increasing diameter (2, 2.8, and 3.25 mm), tapping was performed for the first 5 to 6 mm, and two distractor base plugs were placed at the base of the osteotomies with a repositioning tool. An intraosseous distraction implant was then inserted and 2 inward vertical cuts were made in the bone to allow proper distraction to take place. Correct functioning of the device was checked by distracting the bone fragment 1 mm using the axial distraction screw. A latency distraction healing screw was inserted in each of the distraction implants and the area was left to heal for 5 days. Once primary healing had occurred, the distraction of the newly formed bone callus was activated each day for 10 days (1 mm per day). At the end of the distraction period a final distraction screw was left in place and a final healing screw was inserted. During this time there were no complications and the patient on no occasion complained of discomfort. The distractor device was removed 30 days later, leaving the base plugs in place. One month later a vertical augmentation of 7 mm had been achieved; the base plugs were removed, 3 intraosseous implants were inserted, and a biopsy of the newly formed tissue was obtained. Histologic evaluation of the biopsy specimen showed woven bone formation approximately 75 days after the initial procedure.

Adult

Effect of treatment on cyclosporine- and nifedipine-induced gingival enlargement: clinical and histologic results.

The clinical and histologic responses to periodontal treatment of gingival enlargements, induced when cyclosporine and nifedipine were administered singly or in combination, were evaluated. A significant correlation was noted between plaque, gingivitis, and gingival overgrowth. Severity of enlargement appeared to be greater in patients on combined therapy. All treatment approaches such as scaling, root planning, gingivectomy, and periodontal flaps, and a combination of therapies, were effective in the management of gingival overgrowth up to 1 year after completion of treatment. Adjunctive use of chlorhexidine was found to be beneficial.

Adult

RNA transport in dendrites: a cis-acting targeting element is contained within neuronal BC1 RNA.

In nerve cells, a select group of RNAs has been localized to dendritic domains. Here we have examined dendritic RNA transport in sympathetic neurons in primary culture, using a microinjection protocol with neuronal BC1 RNA and with BC1-derived sequence segments. After cytoplasmic microinjection, full-length BC1 RNA was selectively transported to dendrites; in contrast, control RNAs such as nuclear RNAs and random-sequence irrelevant RNAs remained restricted to cytoplasmic areas proximal to the injection sites. Chimeric RNAs were constructed that contained the full-length BC1 sequence inserted upstream or downstream of the coding regions of nondendritic mRNAs. After microinjection, such chimeric RNAs were specifically targeted to dendrites; microinjected corresponding nonchimeric mRNAs were not. Dendritic transport of BC1 RNA was rapid: the average dendritic delivery rate within the first hour after microinjection was 242 +/- 25 microm/hr. Whereas a 5'-BC1 segment of 62 nucleotides was transported to dendrites to extents and at levels similar to full-length BC1 RNA, a 3'-BC1 segment of 60 nucleotides did not exit injected somata to any significant degree. A cis-acting dendritic targeting element is thus contained in the 5' part of neuronal BC1 RNA. These results demonstrate that mechanisms exist in neurons for fast and specific transport of selected RNAs to dendrites.

Animals

Regenerative periodontal therapy in mucogingival surgery for root coverage.

This article illustrates the potential benefits of regenerative periodontal therapy in mucogingival surgery and esthetic dental treatment. Cases are described in which the treatment of soft-tissue recessions and root exposures are treated with surgical procedures where both clinical soft-tissue augmentation and the regeneration of periodontal attachment are obtained. Cases are also presented to illustrate the clinical application of guided tissue regeneration. Resorbable and nonresorbable barriers are placed over the root surface and bone and covered by the overlying flap, which allows the selective repopulation of the lesion by progenitor cells and the inhibition of a long junctional epithelium. Emphasis is placed on regenerative procedures in soft-tissue augmentation, particularly with respect to rationales, techniques, and indications.

Esthetics, Dental

[Effect of saliva on resorption time of some resorbable membranes].

Since resorbable membranes have been introduced their resorption time has been always an important topic of discussion. The current literature does not cover very accurately the contributing factors associated with this biologic process in the oral cavity. The clinical experience shows that the influence of saliva may be an important factor during the resorption of synthetic resorbable membranes. Six experiments are described in this article in which four synthetic resorbable membranes are tested (Vicryl periodontal mesh, Vicryl collagene, Guidor and Resolut). The membranes are plated in Petri dishes precoated with Agar in contact with saliva. Experiment number 1 and 2 demonstrated that saline solution and Agar do not alter the resorption time of the membranes. Experiment 3 and 4 showed that a dilution of saliva to 1:10 and a non diluted saliva accelerate their resorption time of two of the tested membranes. The Vicryl periodontal mesh and the Vicryl collagene disappeared respectively after 7 and 9 days of contact with the not diluted saliva and after 10 and 12 days of contact with the 1:10 diluted saliva. The experiment 5 and 6 indicated that both salivas (diluted and not diluted) deprived of bacteria do not alter the resorption time of the membranes. In conclusion the pattern of resorption of the synthetic membranes, in this in vitro study, is recognized in the contact between the membrane and the bacterial enzymes present in saliva, and in the mechanical structure of the membrane design.

Absorption

[Course and regression of HELLP syndrome].

The authors reported the outcome of a retrospective study of patients with HELLP syndrome to verify whether this pathology is a clinical entity with models of evolution, regression and well defined treatment. A total of 20 individual cases were reported at the Division of Obstetrics and Gynecology at the G. Gaslini Institute during the period from January 1990 to September 1995. All cases showed the normalisation of the various clinical and hematochemical parameters within a maximum of 8 days after birth. The rapid diagnosis, the immediate extraction of the fetus and appropriate medical treatment led to the prompt resolution of this severe pathology without the need for demolitive surgery frequently used in the past.

Adult

Surgical correction of gingival recessions associated with radicular carious lesions.

In this clinical report, six cases are presented in which radicular carious lesions and gingival recessions were treated concurrently. The combined treatment included the removal of caries, radicular planing, and various surgical techniques for root coverage. Traditional procedures, as well as newer procedures, such as guided tissue regeneration, showed successful results.

Adult

Using a resin-ionomer in guided tissue regenerative procedures: technique and application--case reports.

The purpose of these case reports is to introduce a potential use for a resin-ionomer in guided tissue regeneration. The resin-ionomer is used in a two-fold manner (1) to lute an e-PTFE membrane in place in furcation defects and (2) to act as a barrier itself in furcation and infrabony defects. Clinical observations made in this report indicate that a resin-ionomer barrier may be placed subgingivally without sutures. In addition, the resin-ionomer, given its ability to release fluoride, may serve as a delivery system for local antimicrobial therapy during the initial stages of wound healing. Further studies should be conducted in order to evaluate the potential of the resin-ionomer for the uses described in this article.

Glass Ionomer Cements

Root surface biomodification using a dentin bonding conditioner.

OBJECTIVE: The purpose of this investigation was to compare by scanning electron microscopy (SEM) the surfaces of roots treated with a dentin conditioning agent and other materials that have been used routinely in periodontal therapy for root surface demineralization, namely, citric acid and tetracycline hydrochloride. MATERIALS AND METHODS: Sixteen freshly extracted human teeth were randomly divided into four equal groups; each tooth was hand scaled and planed prior to root surface conditioning with citric acid, tetracycline hydrochloride, and a dentin bonding conditioner; Tenure Dentin Conditioner (DenMat Corporation, Santa Maria, CA). The last has been used previously in conjunction with a resin-ionomer barrier in cases involving guided tissue regeneration. Root specimens were prepared for scanning electron microscopy and representative micrographs were taken at 1000x and 2000x. RESULTS: The results of this study indicate that there are morphological similarities between surfaces obtained with the dentin conditioning agent and the other acidic materials that are used routinely in periodontal regenerative therapy. CONCLUSIONS: Root surface demineralization has been shown to remove the smear layer, and provides a surface which is conductive to mechanical adhesion and cellular mechanisms for growth and attachment.

Acid Etching, Dental

Role of mouse polyomavirus late region in the control of viral DNA replication: a review.

The genome of polyomaviruses is divided into two coding regions: the early and the late region. A relatively short regulatory sequence, encompassing the origin of viral DNA replication (ori), separates the two regions encoding the structural genes. In mouse polyomavirus (Py) in particular, the early DNA codes for three antigens: large, middle and small T-antigen (L-T, M-T and S-T, respectively). Large T antigen binds ori and thus regulates both viral DNA transcription and replication. Middle T antigen has been shown to mediate malignant transformation in non-permissive cells in vitro. No defined function has been assigned to the small T antigen although this gene product is thought to act synergistically both with L- and M-T antigens. The viral late region of Py encodes also three different genes whose products form the viral capsid during the productive infection cycle in permissive cells. Py early region was thought to be the only part of the genome necessary to code for proteins of functional and regulatory significance. The viral late region, on the other hand, was for a long time considered a simple reservoir of structural information, since it codes for capsid proteins and was supposedly devoid of functional control properties. This short review is focused on recent works from our and other laboratories, reporting evidence that in Py also the late region has a functional role since late sequences are involved in the control of viral DNA replication and in capsid assembly. Results indicating that this might be true for the cognate simian virus SV40 will be also reviewed.

Animals

Periodontal and prosthetic treatment of a cleft lip and palate patient: a case report.

Although cleft lip and palate patients are usually treated by a multidisciplinary team involving physicians and dentists, their periodontal condition may be over-looked. Crowded or malpositioned teeth, hypertrophic gingiva, orthodontic appliances, and prosthetic replacements can impede proper plaque removal and thus perpetuate periodontal disease. It is important to incorporate periodontal treatment into the comprehensive treatment as early as possible. This case report discusses the periodontal surgical procedures involved in eliminating a residual ridge defect and the fitting of the final prosthetic reconstruction. Also, the importance of the identification and management of periodontal conditions characteristic of cleft lip and palate patients before and after surgical, orthodontic, and prosthetic rehabilitation will be emphasized.

Adult

Use of a resin-ionomer in guided tissue regeneration: case reports.

The cases presented in this report illustrate: (1) the potential for a fluoride-releasing resin-ionomer (Geristore) to be used as a sealant between an e-PTFE membrane (Gore-Tex) and tooth structure during a guided tissue regeneration, and (2) the potential for a resin-ionomer to function as a barrier in guided tissue regeneration. In the first case, the e-PTFE membrane was trimmed to only cover the area to be treated prior to its placement over the lesion. The resin-ionomer was mixed following manufacturers specifications, placed on four corners of the pre-trimmed membrane and luted into place. In the second case, the resin-ionomer alone was placed over the lesion, teased off the lesion with a curette while in a semi-set state, and luted into place with freshly mixed material after it set completely. These cases provide preliminary clinical evidence that a resin-ionomer may be used subgingivally for guided tissue regeneration.

Adult

Predictive factors of delayed emesis in cisplatin-treated patients and antiemetic activity and tolerability of metoclopramide or dexamethasone. A randomized single-blind study.

To prevent delayed emesis induced by cisplatin (mean dose 90 mg/m2), 120 consecutive patients were randomized to receive, in a 7-day crossover design, oral metoclopramide (20 mg q.i.d.), dexamethasone (1 mg q.i.d.) or placebo (two tablets q.i.d.) starting 24 hours after the end of chemotherapy. Complete protection from nausea, but not from vomiting. was significantly increased by both dexamethasone and metoclopramide with respect to placebo. Important prognostic factors favoring the appearance of delayed emesis were incomplete protection from vomiting during the first 24 hours after cisplatin, female gender, and highest cisplatin doses. Tolerability of both drugs was good. Larger and randomized controlled trials are necessary to identify better preventive treatment of delayed emesis induced by cisplatin.

Cisplatin