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Biomedical subjects

E S Hansen

Publications and source records attributed to E S Hansen.

At least 37 records · Page 2Linked to original sources
PubMed1999

Recombinant bone morphogenetic protein-7 as an intracorporal bone growth stimulator in unstable thoracolumbar burst fractures in humans: preliminary results.

The study presented here is a pilot study in five patients with unstable thoracolumbar spine fractures treated with transpedicular OP-1 transplantation, short segment instrumentation and posterolateral fusion. Recombinant bone morphogenetic protein-7 in combination with a collagen carrier, also referred to as OP-1, has demonstrated ability to induce healing in long-bone segmental defects in dogs, rabbits and monkeys and to induce successful posterolateral spinal fusion in dogs without need for autogenous bone graft. Furthermore OP-1 has been demonstrated to be effective as a bone graft substitute when performing the PLIF maneuver in a sheep model. Five patients with single-level unstable burst fracture and no neurological impairment were treated with intracorporal OP-1 transplantation, posterior fixation (USS) and posterolateral fusion. One patient with osteomalacia and an L2 burst fracture had an additional intracorporal transplantation performed proximal to the instrumented segment, i.e. OP-1 into T 12 and autogenous bone into T 11. Follow-up time was 12-18 months. On serial radiographs, Cobb and kyphotic angles, as well as anterior, middle and posterior column heights, were measured. Serial CT scans were performed to determine the bone mineral density at fracture level. In one case, radiographic and CT evaluation after 3 and 6 months showed severe resorption at the site of transplantation, but after 12 months, new bone had started to fill in at the area of resorption. In all cases there was loss of correction with regard to anterior and middle column height and sagittal balance at the latest follow-up. These preliminary results regarding OP-1 as a bone graft substitute and stimulator of new bone formation have been disappointing, as the OP-1 device in this study was not capable of inducing an early sufficient structural bone support. There are indications to suggest that OP-1 application to a fracture site in humans might result in detrimental enhanced bone resorption as a primary event.

Adult↗
PubMed1999

Chorioamniotic membranes constitute a competent barrier to group b streptococcus in vitro.

OBJECTIVE: To study the penetration of group B streptococcus (GBS) through human chorioamniotic membranes in vitro. STUDY DESIGN: Chorioamniotic membranes from seventeen healthy women were mounted onto glass cylinders and placed in tissue culture trays constituting a two-compartment system with a maternal compartment internally and a fetal compartment externally. GBS from healthy pregnant women and from newborn babies with sepsis were added to the maternal compartment at densities from 10(7) to 10(9) colony forming units (cfu) per ml. RESULTS: Irrespective of inoculum density, GBS was not recovered from the fetal compartment within a 20 h incubation period. By histology, micro-colonies of GBS were found on the maternal surface after 8 h, but invasion of the morphologically intact membranes was not observed. A five log reduction in cfu occurred in the maternal compartment with amnion when GBS were suspended in saline. CONCLUSION: In this in vitro model the membranes appear to constitute an effective barrier against ascending infection.

Amnion↗
PubMed1999

Mortality and causes of death among Danish medical doctors 1973-1992.

BACKGROUND: To examine the mortality pattern of Danish doctors for the period 1973-1992. METHODS: A historical prospective cohort study based on the membership register of the Danish Medical Association. The study population consisted of 21,943 medical doctors, 6012 of whom were women. The doctors' cause-specific mortality was compared with that of the general population. RESULTS: The study covered about 277,000 person-years. A total of 2387 deaths occurred from 1 January 1973 to 31 December 1992. The doctors' mortality was lower than that of the general population. Both sexes showed a standardized mortality ratio (SMR) below one for cancer, circulatory diseases and other natural causes. Mortality due to lung cancer was particularly low. The SMR for suicide was significantly increased, 1.6 for males (95% CI: 1.4-1.9) and 1.7 for females (95% CI: 1.1-2.5). The suicide rate was increased, in particular because of an increased number of suicides by poisoning. In addition female doctors displayed a relatively high mortality due to accidents and other types of violent death. CONCLUSIONS: Compared with the general population the doctors' mortality was low, but the mortality from external causes was increased, mainly due to an excess number of suicides.

Adult↗
PubMed1999

Decrease in enkephalin levels in psoriatic lesions after calcipotriol and mometasone furoate treatment.

BACKGROUND: Enkephalins are opioid peptides that can modulate immune responses and inflammatory processes. Furthermore, they inhibit keratinocyte proliferation/differentiation in vitro. Previously, we have shown that enkephalins are present in increased amounts in lesional psoriasis. OBJECTIVE: To determine the effect of topical treatment with the vitamin D analogue calcipotriol and the corticosteroid mometasone furoate on the level of methionine-enkephalin (enk) in psoriatic lesions. METHODS: Twelve psoriatic patients were treated with calcipotriol and mometasone furoate for 14 days without or with hydrocolloid occlusion. Keratome biopsies were obtained from treated and untreated skin, and the extracted enk was quantified by radioimmunoassay. Furthermore, punch biopsies were obtained for immunohistochemical analysis. RESULTS: Clinically, both calcipotriol and mometasone furoate improved psoriasis to the same degree, the effects being more pronounced after occlusion. Histologically, treatment with mometasone furoate without occlusion decreased both the epidermal thickness/parakeratosis and the number of dermal immunocompetent cells (CD3- and CD68-positive cells). In contrast, treatment with calcipotriol without occlusion reduced the epidermal thickness and the degree of parakeratosis but decreased the number of CD3- and CD68-positive cells only slightly. The mean enk level was decreased by 26 and 86% by calcipotriol without and with occlusion and by 16 and 63% by mometasone furoate without and with occlusion, respectively. The decreases in the enk levels corresponded to the degree of clinical improvement but not to the histological changes. CONCLUSION: The increased levels of enk in psoriatic lesions are reduced in parallel with the clinical improvement induced by a topical vitamin D analogue and a corticosteroid. Because enkephalins can modulate epidermal differentiation and inflammatory processes, the findings indicate that enkephalins may play a role in the pathogenesis of psoriasis.

Administration, Topical↗
PubMed1999

Reperfusion pattern of the immature femoral head after critical ischemia: a microsphere study in pigs.

The topographic reperfusion pattern of the femoral head after critical ischemia has not yet been investigated. We determined the blood flow of the porcine hip regions with the femoral head epiphysis divided into 24 subregions by the tracer microsphere technique. Blood flow was measured under steady-state conditions, at the end of a 6-hour increase in intracapsular hip joint pressure to 250 mm Hg, and 4 hours after release of the joint tamponade. Total femoral head epiphyseal blood flow decreased with ischemia and regained steady-state perfusion after tamponade. The reperfusion pattern of the femoral head epiphysis appeared identical with that of the steady state before ischemia. However, 2 of the 11 experimental epiphyses remained ischemic in the reperfusion phase. We conclude that hip joint tamponade above the arterial pressure level for 6 hours causes global ischemia in the femoral head epiphysis in the immature pig, without regional differences in reperfusion, and that reperfusion occurs at a level like that of the steady state before ischemia.

Age Factors↗
PubMed1999

Functional outcome after partial reduction and 360 degree fusion in grade III-V spondylolisthesis in adolescent and adult patients.

Reduction of high-grade spondylolisthesis is a surgically demanding procedure, which has been reported to incur a relatively high risk of neurologic damage, pseudoarthrosis, and other complications. The aim of this study was to evaluate the radiographic and functional outcome in spondylolisthesis grade III-V, surgically treated with posterior decompression, L4- or L5-S1 CD instrumentation, partial reduction, and posterolateral fusion followed by anterior interbody fusion L5-S1 ad modum Kellogg-Speed. Thirteen patients, mean age 23.8 (range, 11-33) years were followed for mean 28.5 (range, 19-52) months. The anterior slip, lumbosacral angle, sagittal rotation angle and L4/SI angle were significantly improved from preoperatively to follow-up. No patients had neurologic, vascular, thromboembolic, or lung complications. Twelve patients reported a better or unchanged (3 patients) physical and emotional status. At follow-up, 10 patients were in full-time jobs or back to school. The method was safe and provided a stable rigid fixation with partial reduction of the deformity and a satisfactory functional outcome.

Adolescent↗
PubMed1999

A review of ceramic coatings for implant fixation.

The present series of eight studies was performed in order to investigate the effect of various clinically relevant factors on bone ingrowth in relation to hydroxyapatite (HA) and titanium-alloy (Ti) coating when subjected to pathological and mechanical conditions mimicking the clinical situation. HA- and Ti-coated implants were inserted into the femoral condyles of mature dogs and one study was performed on humans. The observation period ranged from 4 to 52 weeks, and the results were evaluated by mechanical push-out testing, histomorphometric analysis, polarized light microscopy, UV fluorescence microscopy and collagen analysis. There were no complications related to the operative procedures, and all dogs were killed according to the original time schedule. Two studies focused on in vivo mechanisms and factors influencing resorption of HA coating. The overall conclusions from these studies are that HA coatings do resorb in vivo, that micromotion accelerates resorption, and that resorbed HA is partly replaced by newly formed bone, suggesting that implants fixation is durable. The other studies focused on the significance of mechanical stabilization and loading conditions of the implant immediately after surgery. From these studies, it can be concluded that HA-coating has a positive effect on bone-implant fixation in various situations, i.e., under stable loaded conditions and under unstable mechanical conditions. The most striking effect of HA coating was that it enhanced bone growth across a gap around the implant both during stable and unstable mechanical conditions; it even converted a motion-induced fibrous membrane to bony anchorage.

Animals↗
PubMed1998

Increased levels of enkephalin following natural sunlight (combined with salt water bathing at the Dead Sea) and ultraviolet A irradiation.

The opioid peptides enkephalins have been shown to modulate inflammatory responses and keratinocyte proliferation and differentiation. Furthermore, increased levels of enkephalin are present in psoriatic lesions. The purpose of the present study was to determine the effect of natural sunlight combined with salt water bathing in the Dead Sea on the methionine-enkephalin (e.n.k.) level in psoriatic skin. Ten patients were treated at the Dead Sea for 4 weeks, and keratotome biopsies were obtained before and after treatment. The amount of enkephalin extracted from the biopsies was measured by radioimmunoassay. Treatment at the Dead Sea resulted in a complete clinical clearance of psoriasis, and immunohistochemical stainings of lesional skin showed that the treatment decreased both epidermal thickness/parakeratosis and the dermal infiltration of CD3- and CD68-positive cells, although the number of CD3- and CD68-positive cells became normal in only two of the 10 cases. However, there was only a slight decrease in the mean enk levels (21%). Furthermore, the level of enk was high in non-lesional psoriatic skin after treatment at the Dead Sea, and immunostaining showed that, in some patients, the treatment induced a mild epidermal hyperplasia and a dermal infiltration of CD3- and CD68-positive cells. Enkephalin-like immunoreactivity was detected in the cytoplasm of both epidermal keratinocytes and dermal infiltrating cells. To determine whether the relatively high skin enk levels after treatment at the Dead Sea was caused by ultraviolet (UV) radiation, normal volunteers were exposed to a single dose of UVA and UVB (2 minimal erythema doses). UVA, but not UVB, irradiation stimulated the mean enk level in the irradiated skin by about sixfold. Furthermore, multiple whole-body UVA irradiations not only resulted in increased skin levels of enk, but also in increased plasma levels. In conclusion, natural sunlight combined with salt water bathing cleared psoriasis without causing a significant decrease in lesional enk levels. Furthermore, non-lesional enk levels were increased. These findings may be the result of a direct stimulatory effect of UVA irradiation on enk formation in the skin. It is possible that the increased circulating levels of enk after UV exposure may contribute to the beneficial effects of UVA irradiation.

Adult↗
PubMed1998

Effect of moderate exercise on blood flow in the gastrointestinal tract in trained conscious miniature swine.

AIM: To examine how moderate exercise affects the blood flow in the gastrointestinal tract. MATERIALS AND METHODS: Twelve miniature swine weighing 38-43 kg were used. All animals were trained on a cardiac exercise treadmill. Blood flow measurements were done on conscious animals using labeled microspheres with a diameter of 16.5 +/- 0.1 (SD) microm. The first flow was measured while the animal was awake and resting, the second flow after 15 min of exercise, the third flow after 30 min of rest. RESULTS: Flow in the oesophagus at rest was 19.5 +/- 1.3 (SEM) ml/min/100 g. During exercise the flow decreased to 13.3 +/- 1.2 (SEM) ml/min/100 g (ns). After 30 min of rest the flow was 9.9 +/- 1.2 (SEM) ml/min/100 g (p < 0.05 when comparing the flow before and after exercise). Flow in the cardia at rest was 23.1 +/- 1.3 (SEM) ml/min/100 g. During exercise the flow decreased to 14.0 +/- 1.2 (SEM) ml/min/100 g (p < 0.05). After 30 min of rest the flow was 15.0 +/- 1.2 (SEM) ml/min/100 g. Flow in the pylorus at rest was 38.9 +/- 1.1 (SEM) ml/min/100 g. During exercise the flow decreased to 24.6 +/- 1.1 (SEM) ml/min/100 g (p < 0.01). After 30 min of rest the flow was 26.9 +/- 1.2 (SEM) ml/min/100 g. Blood flow in the small and large intestine was mainly unaffected by moderate exercise. CONCLUSION: Under moderate exercise, blood flow in the upper part of the gastrointestinal tract declines while it is mainly unaffected in the duodenum, small and large intestine.

Analysis of Variance↗
PubMed1997

1997 Volvo Award winner in clinical studies. The effect of pedicle screw instrumentation on functional outcome and fusion rates in posterolateral lumbar spinal fusion: a prospective, randomized clinical study.

STUDY DESIGN: A prospective randomized clinical study. OBJECTIVES: To evaluate supplementary pedicle screw fixation (Cotrel-Dubousset) in posterolateral lumbar spinal fusion. SUMMARY OF BACKGROUND DATA: The rationale behind lumbar fusion is to eliminate pathologic motion to relieve pain. To improve fusion rates and to allow reduction, a rigid transpedicular screw fixation may be beneficial, but the positive effect of this may be counter-balanced by an increase in complications. METHODS: The inclusion criteria were severe, chronic low back pain from spondylolisthesis Grades 1 and 2 or from primary or secondary degenerative segmental instability. One hundred thirty patients were randomly allocated to receive no instrumentation (n = 66) or Cotrel-Dubousset instrumentation (n = 64) in posterolateral lumbar fusion. Variables were registered at the time of surgery and at 1 and 2 years after surgery. RESULTS: Follow-up was achieved in 97.7% of the patients. Fusion rates deduced from plain radiographs were not significantly different between instrumented and noninstrumented groups. The functional outcome assessed by the Dallas Pain Questionnaire improved significantly in both groups, and there were no significant differences in results between the two groups, except for significantly better (P < 0.06) functional outcome in relation to daily activities in the instrumented group when neural decompression had been performed. The global patients' satisfaction was 82% in the instrumented group versus 74% in the noninstrumented group (not significant). Fixation of instrumentation increased operation time, blood loss, and early reoperation rate significantly. Patients experienced only a few minor postoperative complications; none were major. Two infections appeared in the Cotrel-Dubousset group. Significant symptoms from misplacement of pedicle screws were seen in 4.8% of the instrumented patients. CONCLUSIONS: Lumbar posterolateral fusion with pedicle screw fixation increases the operation time, blood loss, and reoperation rate, and leads to a significant risk of nerve injury. The functional outcome improves significantly with high patient satisfaction, with or without instrumentation. No significant differences were observed between the two groups in functional outcome and fusion rate. The only gain in functional outcome from instrumentation was found in the daily activity category in patients with supplementary neural decompression. The results of this study do not justify the general use of pedicle screw fixation alone as an adjunct to posterolateral lumbar fusion.

Adult↗
PubMed1997

Spinal cord and nerve root blood flow in acute double level spinal stenosis.

STUDY DESIGN: Twenty-four pigs were randomized into three groups of eight pigs; a control group with 0% stenosis, a 25% stenosis group, and a 50% stenosis group. A fourth 75% stenosis group was added when results of the randomized experiment had been analyzed. Blood flow of the spinal cord and nerve roots and spinal evoked potentials were determined before and 1 hour after induction of the spinal stenoses. OBJECTIVES: To study the acute effects of different degrees of spinal stenosis on neural tissue blood flow and spinal evoked potentials. SUMMARY OF BACKGROUND DATA: Spinal cord dysfunction may be caused by vascular impairment or mechanical injury to neural tissue. Experimental double level compression of the cauda equina causes reversible nerve root edema, stasis, blood flow decrease, and compromised neural function. The vascular pathophysiology after spinal cord trauma was studied previously, and both increased and decreased neural tissue blood flow have been reported. METHODS: Two level spinal stenosis was introduced by placement of stenosing bands around the dural sac at L4 and L6. Neurologic function was monitored by sensory and motor evoked potentials. Regional blood flow (RBF) was measured in the stenotic segments between the bands and other regions of neural tissue by radioactive microspheres before and after induction of stenosis. RESULTS: Regional blood flow increased in the stenotic segments after 0% sham stenosis. Analysis of variance revealed no differences in RBF between the three randomized groups under comparable conditions of 0% stenosis. However, the RBF level of the added 75% group was lower than that of the other three groups. By comparison of RBF within groups before and after stenosis, no decrease in RBF was found between the stenosing bands in any of the groups. Fifty percent stenosis changed the amplitude of evoked potentials in half of the animals. Seventy-five percent stenosis caused severe changes in evoked potentials in 7 of 8 animals. CONCLUSIONS: Blood supply of the spinal cord and nerve roots in the segments between two central stenoses is preserved immediately after stenosis introduction by way of the segmental nerve pathway, even if nerve conduction is impaired.

Acute Disease↗
PubMed1997

[Mortality and cause of death among Danish physicians 1973-1992].

This study examines mortality rates of Danish doctors and describes pattern and causes of death for the period 1973-1992. The study comprises 21,943 medical doctors, 6012 of whom were women. At the end of 1992 there were 2387 recorded deaths. The doctors had lower mortality rates than the general population. A significant lower mortality was seen for male medical specialists compared to general practitioners. A gender-difference was seen among the youngest doctors with the female doctors suffering a considerably higher mortality than the male doctors did. Both sexes showed SMR below unity for cancer, circulatory diseases and other natural causes. Mortality due to lung cancer was particularly low. The suicide mortality was increased for both sexes, in particular because of an increased number of suicides by poisoning. Compared with the general population the doctors' mortality was low, but the mortality from external causes was increased, mainly due to an excess number of suicides.

Cause of Death↗
PubMed1997

A cohort mortality study of foundry workers.

Since the 1970s, hygienic improvements have led to a reduction in the level of airborne pollutants in Danish foundries. This mortality study reflects the exposure situation prior to 1970, and the findings may be used as a baseline for future evaluations of the preventive impact of reduced exposure. Mortality data were derived from a historical cohort study in which 3,056 foundry workers were compared with 43,024 workers employed in other industries. The foundry workers' life-long risk of dying from pneumoconioses averaged 2% and the corresponding standardized mortality ratio (SMR) equaled 7,368 (95% confidence interval (95% CI): 4,029-12,363). Excess mortality was also seen for chronic bronchitis and emphysema (SMR = 132, 95% CI: 98-185). Nonsignificant increases were seen for buccal cancer, stomach cancer, colon cancer, and urothelial cancer. In conclusion, Danish foundry workers exposed prior to 1970 seem to suffer an excess risk of devastating lung disease of occupational origin.

Adolescent↗
PubMed1997

Microbiology of semen specimens from males attending a fertility clinic.

The relationship between semen quality, pyospermia and bacteriology was studied in 201 semen specimens from male patients attending a fertility clinic. Semen quality parameters were within normal limits in 115 (57%) patients, slightly reduced in 60 (30%), and 26 (13%) had findings indicating reduced fertility. Twelve patients (6%) had pyospermia. In 182 patients, 552 microorganisms were detected, including Enterobacteriaceae (2.8%), Gardnerella vaginalis (9.6%), Chlamydia trachomatis (1.6%), Mycoplasma genitalium (0.9%), and Ureaplasma urealyticum (11.8%). Semen quality was neither related to occurrence of microorganisms nor pyospermia. However, pyospermia was associated with simultaneous growth of Gardnerella vaginalis and Ureaplasma urealyticum. The exact nature of this association could not be ascertained, in as far as the males were not questioned about urethritis symptoms.

Adult↗
PubMed1997

Pyospermia and preterm, prelabor, rupture of membranes.

OBJECTIVES: To investigate the possible association between presence of clinically unrecognized, specific genito-urinary tract microorganisms in men and preterm, prelabor rupture of membranes (PPROM) in their spouses. STUDY DESIGN: Case control study. Eleven couples with PPROM were enrolled in the case group and 18 couples with normal pregnancies in the control group. For each man, samples of urine and semen were collected and a urethra swab was obtained. Microbiological assessment included, for each woman, cultures from vagina/cervix, urine and placenta. A smear from the vagina was obtained. RESULTS: In the case group, pyospermia was found in three men. Two of these men, as well as their spouses, were Chlamydia trachomatis positive, and in the third man no microorganisms could be detected. None of the controls had pyospermia (p = 0.045). Among the eight cases without pyospermia one man and one women with Chlamydia trachomatis was found, but their spouses were negative. In the control group, no Chlamydia trachomatis was detected (p = 0.014). Among the women two cases and one control had positive culture of Group B streptococci (NS). CONCLUSION: We suggest that the genital microflora of the man is associated with PPROM.

Adult↗
PubMed1997

Vasorelaxation in isolated bone arteries. Vasoactive intestinal peptide, substance P, calcitonin gene-related peptide, and bradykinin studied in pigs.

We assessed the effects of vasoactive intestinal peptide (VIP), calcitonin gene-related peptide (CGRP), substance P (SP), and bradykinin in arteries (diameter approximately 230 microns) isolated from cancellous bone from pigs. Arterial segments (2 mm long) were mounted on a myograph for measurement of isometric force development. After submaximal precontraction with norepinephrine, VIP (10(-10)-10(-7) M), CGRP (10(-11)-10(-7) M), SP (10(-6) M), and bradykinin (10(-11)-10(-6) M) were added. 44 arterial segments (23 pigs) were investigated. VIP-, CGRP-, and bradykinin induced a concentration-dependent vasorelaxation, while SP mediated a transient relaxation. After mechanical removal of the endothelium, the effects of SP and bradykinin were completely abolished, while the relaxation to CGRP was still pronounced. This indicates that the effects of SP and bradykinin are mediated by the endothelium, while CGRP mainly mediates relaxation by a direct effect on vascular smooth muscle cells. The relaxations to CGRP and bradykinin were still significant after inhibition of nitric oxide synthase with 10(-4) M N omega-nitro-L-arginine (L-NNA) and inhibition of prostaglandin synthesis with 10(-5) M indomethacin, indicating the existence of an alternative vasorelaxing pathway. Our findings support the theory of a vasoregulatory role of neuropeptides in bone.

Animals↗