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Biomedical subjects

E Rousseau

Publications and source records attributed to E Rousseau.

At least 109 records · Page 6Linked to original sources
PubMed1982

Arthritis: preferred learning methods among Arizona therapists.

Arizona occupational and physical therapists were surveyed about their needs for education in arthritis. Responses to statements regarding attitudes, knowledge, willingness to learn, and preferred methods of learning were subjected to chi-square analysis. The results showed that a significant number of both occupational and physical therapists are deficient in knowledge about the disease and maintain negative attitudes about patients with rheumatoid arthritis. Both groups indicated a high interest in continuing education about arthritis. The preferred method of learning was self-study guides.

Arthritis↗
PubMed1981

Results of a survey of pediatric clerkship programs in American medical schools.

Pediatric clerkship coordinators from 119 medical schools responded to a questionnaire about administration, resources, goals, instructional design, evaluation, and perceived strengths and weaknesses of their own programs. Problems were reported in the definition of objectives, the evaluation of students' knowledge, interviewing and physical-examination skills, and problem-solving ability. A dedicated faculty and house staff interested in teaching and a large and varied patient population were perceived as strengths of many programs. Weaknesses cited included a lack of uniformity in students' experience, insufficient time spent on the pediatric clerkship, too many students with decreased faculty contact, overemphasis on tertiary care, an inadequate ambulatory experience, lack of specific objectives, and unsystematic evaluation methods. It is hoped that this survey will provide an impetus for further fruitful exchanges among clerkship directors.

Canada↗
PubMedDate not supplied

Biochemical characterization of the Ca2+ release channel of skeletal and cardiac sarcoplasmic reticulum.

Rapid mixing-vesicle ion flux and planar lipid bilayer-single channel measurements have shown that a high-conductance, ligand-gated Ca2+ release channel is present in 'heavy', junctional-derived membrane fractions of skeletal and cardiac muscle sarcoplasmic reticulum. Using the release channel-specific probe, ryanodine, a 30S protein complex composed of polypeptides of Mr approximately 400,000 has been isolated from cardiac and skeletal muscle. Reconstitution of the complex into planar lipid bilayers has revealed a Ca2+ conductance with properties characteristic of the native Ca2+ release channel.

Adenine Nucleotides↗
PubMedDate not supplied

DNA hybridization for detection of human cytomegalovirus in bronchoalveolar lavage and pharynx biopsy.

A simplified hybridization procedure was used for detection of cytomegalovirus (CMV) in human specimens. The probe was a 32P-labelled cloned DNA fragment of CMV strain AD169. This probe did not hybridize to DNA from uninfected cells or other herpesviruses (herpes simplex virus, Epstein-Barr virus). Specific hybridization was obtained with unselected bronchoalveolar lavage specimens, but the sensitivity of the test (33%) was lower than that of culture (80%) and immunofluorescence (60%) assays which are routinely performed in our laboratory. The detection procedure was also carried out with pharynx biopsy specimens which had been kept frozen at -70 degrees C. CMV DNA was detected in 14% of tumour specimens and only in 1.7% of control specimens (p less than 0.05). The indications of DNA hybridization for CMV diagnosis are discussed.

Bronchoalveolar Lavage Fluid↗
PubMedDate not supplied

BK virus (BKV) detection in urine specimens of immunocompromised patients: comparison between the DNA-DNA hybridization assay, the immunofluorescence test and the dot enzyme immunoassay.

Three methods used for the detection of BK virus in urine specimens, the indirect immunofluorescence test, the dot enzyme immunoassay and the DNA-DNA hybridization assay, were compared by testing specimens from 49 immunocompromised patients. All three assays were effective in detecting BK virus. The technical advantage of each of them was discussed. The immunofluorescence test was found to be the simplest one to perform; the DNA-DNA hybridization assay displayed exquisite sensitivity; and the easy reading of the dot enzyme immunoassay did not require the specialized training inherent to immunofluorescence assays. The dot enzyme immunoassay might therefore be the most practical method for screening urine specimens of immunocompromised patients, especially when the sediment is poor in cells. Conversely, the indirect immunofluorescence test might be the method of choice for checking patients with haemorragic cystitis whose urine samples usually contain large amounts of cells.

BK Virus↗
PubMedDate not supplied

Comparison of CHAPS-induced current fluctuations with sarcoplasmic reticulum Ca2+ release channel activity.

The process of purifying and reconstituting transport membrane proteins generally involves the use of detergents, which often cannot be completely separated from the proteins. The effects of the zwitterionic detergent CHAPS on planar lipid bilayers have been measured, and it is demonstrated that CHAPS can induce microscopic electrical activity in the bilayers. Typical CHAPS-induced activity consists of large current bursts, often separated by intervals of quiescent activity, with no definable conductance levels. The size of the current bursts is generally increased by higher CHAPS concentration or by millimolar ATP and usually reduced by millimolar Mg2+ and micromolar ruthenium red. The response of the CHAPS-induced currents to these agents is compared to that of the ligand-gated Ca2+ release channel of muscle sarcoplasmic reticulum.

Animals↗
PubMedDate not supplied

[Considerations relating to multiple primary carcinomas of the urinary tract (author's transl)].

The patient with more than one primary malignant neoplasm has ceased to be a pathologic curiosity and has instead become a practical problem. It is possible for a patient to have two simultaneous developping independant cancers, but is also possible for a second primary cancer to develop in a cancer prone individual after the initial malignant lesion has been removed by radical surgical procedures. The generally improved survival rate after treatment of cancer has permitted more patients to live long enough for a second or even a third primary lesion to develop and it probably also reflects the more thorough pathological studies in both surgical and autopsy cases. It may be hoped that factors of genetic predisposition, ethiology and pathogenesis that are obscure in the patient with single lesions may be brought out in patients with multiple cancers. We present the case of a 83 years old patient with three different primary malignancies. His first tumour was a clear cell carcinoma of the left kidney at the age of 71, followed by a transitional cell carcinoma gr. II/IV of the bladder seven years later, and followed by a third one two and a half years latter which was a distinct adeno carcinoma of the prostate.

Adenocarcinoma↗